In situ microscopic observation of chitin and fungal cells with chitinous cell walls in hydrothermal conditions

Recent findings of intact chitin in fossil records suggest surprisingly high recalcitrance of this biopolymer during hydrothermal treatments. We also know in the experience of everyday life that mushroom, cells of which have chitinous cell walls, do not fall apart however long they are simmered. We used in situ optical microscopy to examine chitin and fungal cells with chitinous cell walls during hydrothermal treatments and obtained direct evidence that they remained undegraded at temperatures well over 200 °C. The results show very hot and compressed water is needed to make mushrooms mushy.

• Publication year

  2015

• Venue

  Scientific Reports

• Publication date

  2015-07-07

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1038/srep11907PMID 26148792PMCID 4493705
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Poriferan Chitin as a Versatile Template for Extreme Biomimetics

  2015cited by this paper
• Two-way traffic of glycoside hydrolase family 18 processive chitinases on crystalline chitin

  2014cited by this paper
• Identification of chitin in 200-million-year-old gastropod egg capsules

  2014cited by this paper
• Synthesis of nanostructured chitin–hematite composites under extreme biomimetic conditions

  2014cited by this paper
• An extreme biomimetic approach: hydrothermal synthesis of β-chitin/ZnO nanostructured composites.

  2013cited by this paper
• Discovery of 505-million-year old chitin in the basal demosponge Vauxia gracilenta

  2013cited by this paper
• Bacterial chitin degradation—mechanisms and ecophysiological strategies

  2013cited by this paper
• Extreme Biomimetics: formation of zirconium dioxide nanophase using chitinous scaffolds under hydrothermal conditions.

  2013cited by this paper
• Preparation of chitin-silica composites by in vitro silicification of two-dimensional Ianthella basta demosponge chitinous scaffolds under modified Stöber conditions.

  2013cited by this paper
• Shape-programmed folding of stimuli-responsive polymer bilayers.

  2012cited by this paper
• NIH Image to ImageJ: 25 years of image analysis

  2012cited by this paper
• Chitin Nanostructures in Living Organisms

  2011cited by this paper
• The Importance of Chitin in the Marine Environment

  2011cited by this paper
• Characterization of Organics Consistent with β-Chitin Preserved in the Late Eocene Cuttlefish Mississaepia mississippiensis

  2011cited by this paper
• Applications of Chitin and Its Derivatives in Biological Medicine

  2010cited by this paper
• Chitin and chitosan polymers: Chemistry, solubility and fiber formation

  2009cited by this paper
• Thermogravimetric analysis of chitins of different origin

  2008cited by this paper
• Crystalline-to-amorphous transformation of cellulose in hot and compressed water and its implications for hydrothermal conversion

  2008cited by this paper
• Cell proliferation at 122°C and isotopically heavy CH4 production by a hyperthermophilic methanogen under high-pressure cultivation

  2008cited by this paper
• FOSSIL DIAGENESIS IN THE BURGESS SHALE

  2007cited by this paper
• Kinetics of winter mushrooms (Flammulina velutipes) microstructure and quality changes during thermal processing

  2007cited by this paper
• Cell structure degradation in Escherichia coli and Thermococcus sp. strain Tc-1-95 associated with thermal death resulting from brief heat treatment.

  2006cited by this paper
• Viscosity measurements of water at high temperatures and pressures using dynamic light scattering.

  2006cited by this paper
• Cooking cellulose in hot and compressed water.

  2006cited by this paper
• A high-temperature and -pressure microscope cell to observe colloidal behaviors in subcritical and supercritical water: Brownian motion of colloids near a wall

  2006cited by this paper
• Cellulose: fascinating biopolymer and sustainable raw material.

  2005cited by this paper
• Production of amino acids from bovine serum albumin by continuous sub-critical water hydrolysis

  2005cited by this paper
• The Microbial Role in Hot Spring Silicification

  2004cited by this paper
• THE ROLE OF DECAY AND MINERALIZATION IN THE PRESERVATION OF SOFT-BODIED FOSSILS

  2003cited by this paper
• Autochthonous eukaryotic diversity in hydrothermal sediment and experimental microcolonizers at the Mid-Atlantic Ridge

  2003cited by this paper
• Flow cell for in situ optical microscopy in water at high temperatures and pressures up to supercritical state

  2002cited by this paper
• Isolation of a highly copper-tolerant yeast, Cryptococcus sp., from the Japan Trench and the induction of superoxide dismutase activity by Cu2+

  2001cited by this paper
• Thermophilic Fungi: Their Physiology and Enzymes

  2000cited by this paper
• Comparison of the Hydrothermal Decomposition Reactivities of Chitin and Cellulose

  1999cited by this paper
• Preservation of Chitin in 25-Million-Year-Old Fossils

  1997cited by this paper
• Brock Biology of Microorganisms

  1996cited by this paper
• Selective preservation of chitin during the decay of shrimp

  1995cited by this paper
• Fungal Cell Wall: Structure, Synthesis, and Assembly

  1991cited by this paper
• Experimental studies in Precambrian paleontology: Structural and chemical changes in blue-green algae during simulated fossilization in synthetic chert

  1976cited by this paper
• Biology of microorganisms

  1970cited by this paper

• Exploring allomelanin: A comparative analysis via natural product extraction and synthesis

  2026cites this paper
• Microwave-enzyme-assisted extraction as an eco-friendly approach for cadmium extraction from edible mushrooms

  2025cites this paper
• Exposed tryptophan residues in the chitin-binding domain of ChiA74 chitinase are important for chitin-binding and antifungal activity.

  2025cites this paper
• Polyethylene glycol infiltrated biomass-derived porous carbon phase change composites for efficient thermal energy storage

  2024cites this paper
• Conversion of lignocellulosic biomass waste into mycoprotein: current status and future directions for sustainable protein production

  2024cites this paper
• Nutritional, Bioactive, and Flavor Components of Giant Stropharia (Stropharia rugoso-annulata): A Review

  2023cites this paper
• Biopolymer-chitin products by direct ink writing (DIW): A review

  2023cites this paper
• Hydrothermal Treatment of Biomass Feedstocks for Sustainable Production of Chemicals, Fuels, and Materials: Progress and Perspectives.

  2023cites this paper
• Pudrición blanca en duramen de ejemplares de Melia azedarach (Meliaceae) del arbolado urbano de La Plata (Buenos Aires): agente causal y caracterización químico-anatómica del leño atacado

  2022cites this paper
• Cu nanoparticles embedded on reticular chitosan-derived N-doped carbon: Application to the catalytic hydrogenation of alkenes, alkynes and N-heteroarenes

  2022cites this paper
• Chitosan mediated gold nanoparticles against pathogenic bacteria, fungal strains and MCF-7 cancer cells.

  2020cites this paper
• Single-molecule imaging analysis reveals the mechanism of a high-catalytic-activity mutant of chitinase A from Serratia marcescens

  2020cites this paper
• Molecular identification of fungi microfossils in a Neoproterozoic shale rock

  2020cites this paper
• Chitin and its derivatives: Structural properties and biomedical applications.

  2020cites this paper
• Combined Approach to Engineer a Highly Active Mutant of Processive Chitinase Hydrolyzing Crystalline Chitin

  2020cites this paper
• Non-catalytic conversion of chitin into chromogen I in high-temperature water.

  2019cites this paper
• Scaffolds for 3D Cell Culture and Cellular Agriculture Applications Derived From Non-animal Sources

  2019cites this paper
• Processive chitinase is Brownian monorail operated by fast catalysis after peeling rail from crystalline chitin

  2018cites this paper
• Recent advances in chitin based materials constructed via physical methods

  2018cites this paper
• Hydrolysis kinetics and mechanism of chitin in subcritical water

  2018cites this paper
• Discovery of chitin in skeletons of non-verongiid Red Sea demosponges

  2018cites this paper
• Identification of a novel chitinase from Aeromonas hydrophila AH‐1N for the degradation of chitin within fungal mycelium

  2018cites this paper
• Chitins and chitinase activity in airway diseases

  2018cites this paper
• First Report on Chitin in a Non-Verongiid Marine Demosponge: The Mycale euplectellioides Case

  2018cites this paper
• Induction of Bacillus cereus chitinases as a response to lysates of Fusarium verticillioides

  2017cites this paper
• Implications of molecular diversity of chitin and its derivatives

  2017cites this paper
• Antifungal Effects of Lipopeptide Produced by Bacillus amyloliquefaciens BH072

  2017cites this paper
• Biodegradation and detoxification of azo solvent dye by ethylene glycol tolerant ligninolytic ascomycete strain of Pseudocochliobolus verruculosus NFCCI 3818

  2017cites this paper
• Comparative In Situ Microscopic Observation of Cellulose and Chitin in Hydrothermal Conditions

  2017cites this paper
• Hydrothermal Synthesis of Advanced Chitin-Based Materials

  2017cites this paper
• Influence of Processing Conditions on the Thermal Stability and Mechanical Properties of PP/Silica-Lignin Composites

  2016cites this paper