ATP-sensitive potassium channels modulate in vitro tocolytic effects of β2-AR agonists on uterine muscle rings in rats in early but not in late pregnancy

Aim To investigate whether ATP-sensitive potassium (KATP) channels modulate the tocolytic effect of β2-AR agonists (ritodrine and salmeterol) in early-pregnant (day 6) and late-pregnant (day 22) rat uterus in vitro, in order to examine the relation between the KATP channel sulphonylurea-binding regulatory subunit (SUR) expression and pharmacological reactivity of β2-AR agonists. Methods The tocolytic effects of ritodrine and salmeterol (10-10-10-5 M) on spontaneous rhythmic contractions were investigated cumulatively, alone, or in the presence of the KATP channel blocker glibenclamide (10-6 M) and the KATP channel opener pinacidil (10-9-10-7 M) after 5-min preincubation. Results β2-AR agonist induced myometrial relaxation was inhibited by glibenclamide and enhanced by pinacidil on day 6, when SUR1 expression levels were high. Neither glibenclamide nor pinacidil mediated tocolytic effect was measured on day 22. Conclusion Low expression of the KATP channels at the end of gestation may facilitate enhanced excitability and contractility in the rat myometrium. The combination of a betamimetic and a KATP channel opener will therefore not be of therapeutic relevance in the treatment of preterm delivery.

• Publication year

  2015

• Venue

  Croatian Medical Journal

• Publication date

  2015-04-01

• Fields of study

  Medicine, Chemistry

• Identifiers
  DOI 10.3325/cmj.2015.56.114PMID 25891870PMCID 4410172
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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