Evaluation of Chromosomal Instability in Diabetic Rats Treated with Naringin

We used the bone marrow DNA strand breaks, micronucleus formations, spermatocyte chromosomal aberrations, and sperm characteristic assays to investigate the chromosomal instability in somatic and germinal cells of diabetic rats treated with multiple doses of naringin. The obtained results revealed that naringin was neither cytotoxic nor genotoxic for the rats at all tested doses. Moreover, naringin significantly reduced the diabetes-induced chromosomal instability in somatic and germinal cells in a dose-dependent manner. In addition, diabetes induced marked biochemical alterations characteristic of oxidative stress including enhanced lipid peroxidation, accumulation of oxidized glutathione, reduction in reduced glutathione, and accumulation of intracellular reactive oxygen species. Treatment with naringin ameliorated these biochemical markers dose-dependently. In conclusion, naringin confers an appealing protective effect against diabetes-induced chromosomal instability towards rat somatic and germinal cells which might be explained partially via diminishing the de novo free radical generation induced by hyperglycemia. Thus, naringin might be a good candidate to reduce genotoxic risk associated with hyperglycemia and may provide decreases in the development of secondary malignancy and abnormal reproductive outcomes risks, which seems especially important for diabetic patients.

• Publication year

  2011

• Venue

  Oxidative Medicine and Cellular Longevity

• Publication date

  2011-09-20

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1155/2011/365292PMID 21941606PMCID 3176618
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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• Health-promoting properties of common herbs.

  1999influential reference
• The role of diabetes mellitus in the aetiology of renal cell cancer

  1999influential reference
• WHO and the International Diabetes Federation: regional partners.

  1999cited by this paper
• Intestinal bacterial metabolism of flavonoids and its relation to some biological activities

  1998cited by this paper
• Effect of diabetes mellitus upon male reproductive function.

  1997cited by this paper
• Repair of alkali-labile sites within the mitochondrial DNA of RINr 38 cells after exposure to the nitrosourea streptozotocin.

  1991cited by this paper
• Comparative interactions of streptozotocin and chlorozotocin with DNA of an insulin-secreting cell line (RINr)

  1986cited by this paper
• Determination of glutathione and glutathione disulfide in biological samples.

  1985cited by this paper
• Dietary carcinogens and anticarcinogens. Oxygen radicals and degenerative diseases.

  1983cited by this paper
• Leydig cell function in streptozotocin-induced diabetic rats

  1979cited by this paper
• Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction.

  1979cited by this paper
• Chemical induction of sperm abnormalities in mice.

  1975cited by this paper
• Diabetogenic action of streptozotocin: relationship of dose to metabolic response.

  1969cited by this paper
• Tissue sulfhydryl groups.

  1959influential reference
• Protein measurement with the Folin phenol reagent.

  1951cited by this paper

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