Low Nitrogen Enhances Nitrogen Use Efficiency by Triggering NO3- Uptake and Its Long-Distance Translocation.

Nitrogen is essential for plant growth and crop productivity; however, nitrogen use efficiency (NUE) decreases with increasing N supply, resulting in a waste of resources. Molecular mechanisms underlying low-nitrogen (LN)-mediated enhancement of NUE are not clear. We used high-NUE Brassica napus genotype H (Xiangyou 15), low-NUE B. napus genotype L (814), and Arabidopsis mutant aux1 to elucidate the mechanism underlying the changes in NUE under different rates of N fertilizer application. NUE of B. napus increased under LN, which enhanced N uptake ability by regulating root system architecture and plasma membrane H+-ATPase activity; AUX1 was involved in this process. Additionally, BnNRT1.5 was upregulated and BnNRT1.8 was downregulated under LN, whereby more N was transferred to the shoot through enhanced N transport. Observed changes in photosynthesis under LN were associated with N assimilation efficiency. Our study provides new insights into the mechanisms of plant adaptation to the environment.

• Publication year

  2019

• Venue

  Journal of Agricultural and Food Chemistry

• Publication date

  2019-06-07

• Fields of study

  Biology, Agricultural and Food Sciences, Chemistry, Environmental Science, Medicine

• Identifiers
  DOI 10.1021/acs.jafc.9b02491PMID 31184154
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Production of Low-Potassium Content Melon Through Hydroponic Nutrient Management Using Perlite Substrate

  2018cited by this paper
• Ethylene mediates dichromate-induced inhibition of primary root growth by altering AUX1 expression and auxin accumulation in Arabidopsis thaliana.

  2018cited by this paper
• AUX1-mediated root hair auxin influx governs SCFTIR1/AFB-type Ca2+ signaling

  2018cited by this paper
• The auxin influx carrier, OsAUX3, regulates rice root development and responses to aluminium stress.

  2018cited by this paper
• Leaf Amino Acid Supply Affects Photosynthetic and Plant Nitrogen Use Efficiency under Nitrogen Stress1[OPEN]

  2018cited by this paper
• Genomics-Assisted Identification and Characterization of the Genetic Variants Underlying Differential Nitrogen Use Efficiencies in Allotetraploid Rapeseed Genotypes

  2018cited by this paper
• Genetic Dissection of Root Morphological Traits Related to Nitrogen Use Efficiency in Brassica napus L. under Two Contrasting Nitrogen Conditions

  2017cited by this paper
• Pitfalls in Root Trait Calculations: How Ignoring Diameter Heterogeneity Can Lead to Overestimation of Functional Traits

  2017cited by this paper
• Lanthanum Inhibits Primary Root Growth by Repressing Auxin Carrier Abundances in Arabidopsis

  2017cited by this paper
• Complementary diversity for nitrogen uptake and utilisation efficiency reveals broad potential for increased sustainability of oilseed rape production

  2016cited by this paper
• Genome-wide analysis of autophagy-associated genes in foxtail millet (Setaria italica L.) and characterization of the function of SiATG8a in conferring tolerance to nitrogen starvation in rice

  2016cited by this paper
• Nitrate Transport, Sensing, and Responses in Plants.

  2016cited by this paper
• Root transcriptome of two contrasting indica rice cultivars uncovers regulators of root development and physiological responses

  2016cited by this paper
• Nitrogen Use Efficiency Is Mediated by Vacuolar Nitrate Sequestration Capacity in Roots of Brassica napus[OPEN]

  2016cited by this paper
• Nutrient deficiencies modify the ionomic composition of plant tissues: a focus on cross-talk between molybdenum and other nutrients in Brassica napus.

  2016cited by this paper
• Auxin Influx Carrier AUX1 Confers Acid Resistance for Arabidopsis Root Elongation Through the Regulation of Plasma Membrane H+-ATPase

  2016cited by this paper
• V-ATPase and V-PPase at the Tonoplast Affect NO3− Content in Brassica napus by Controlling Distribution of NO3− Between the Cytoplasm and Vacuole

  2015cited by this paper
• Nitrate reutilization mechanisms in the tonoplast of two Brassica napus genotypes with different nitrogen use efficiency

  2015cited by this paper
• Reduced frequency of lateral root branching improves N capture from low-N soils in maize

  2015cited by this paper
• The Optimal Lateral Root Branching Density for Maize Depends on Nitrogen and Phosphorus Availability1[C][W][OPEN]

  2014cited by this paper
• Genotypic differences in nitrate uptake, translocation and assimilation of two Chinese cabbage cultivars [Brassica campestris L. ssp. Chinensis (L.)].

  2013cited by this paper
• Steep, cheap and deep: an ideotype to optimize water and N acquisition by maize root systems.

  2013cited by this paper
• Maize root growth angles become steeper under low N conditions

  2013cited by this paper
• Lateral root development in Arabidopsis: fifty shades of auxin.

  2013cited by this paper
• Protocol: High-throughput and quantitative assays of auxin and auxin precursors from minute tissue samples

  2012cited by this paper
• Knockdown of a Rice Stelar Nitrate Transporter Alters Long-Distance Translocation But Not Root Influx1[W][OA]

  2012cited by this paper
• Uptake, allocation and signaling of nitrate.

  2012cited by this paper
• Isoforms of plasma membrane H(+)-ATPase in rice root and shoot are differentially induced by starvation and resupply of NO₃⁻ or NH₄+.

  2011cited by this paper
• Nitrate Transporters and Root Architecture

  2011cited by this paper
• Shoot-supplied ammonium targets the root auxin influx carrier AUX1 and inhibits lateral root emergence in Arabidopsis.

  2011cited by this paper
• Nitrogen Redistribution Characteristics of Oilseed Rape (Brassica napus L.). Varieties with Different Nitrogen-Use Efficiencies during Later Growth Period

  2010cited by this paper
• Molecular Characterization of Mutant Arabidopsis Plants with Reduced Plasma Membrane Proton Pump Activity*

  2010cited by this paper
• The Arabidopsis Nitrate Transporter NRT1.8 Functions in Nitrate Removal from the Xylem Sap and Mediates Cadmium Tolerance[C][W]

  2010cited by this paper
• Arabidopsis V-ATPase activity at the tonoplast is required for efficient nutrient storage but not for sodium accumulation

  2010cited by this paper
• Cadmium accumulation in vetiveria zizanioides and its effects on growth, physiological and biochemical characters.

  2010cited by this paper
• Study on differences of nitrogen efficiency and nitrogen response in different oilseed rape (Brassica napus L.) varieties.

  2009cited by this paper
• Natural variation in Arabidopsis adaptation to growth at low nitrogen conditions.

  2009cited by this paper
• Free IAA in stigmas and styles during pollen germination and pollen tube growth of Nicotiana tabacum.

  2008cited by this paper
• Mutation of the Arabidopsis NRT1.5 Nitrate Transporter Causes Defective Root-to-Shoot Nitrate Transport[W][OA]

  2008cited by this paper
• The challenge of improving nitrogen use efficiency in crop plants: towards a more central role for genetic variability and quantitative genetics within integrated approaches.

  2007cited by this paper
• Comparing nitrate storage and remobilization in two rice cultivars that differ in their nitrogen use efficiency.

  2007cited by this paper
• Boron deficiency decreases plasmalemma H+-ATPase expression and nitrate uptake, and promotes ammonium assimilation into asparagine in tobacco roots

  2007cited by this paper
• Expression of Rice (Oryza sativa L.) Genes Involved in High-Affinity Nitrate Transport during the Period of Nitrate Induction

  2006cited by this paper
• Genome-Wide Reprogramming of Primary and Secondary Metabolism, Protein Synthesis, Cellular Growth Processes, and the Regulatory Infrastructure of Arabidopsis in Response to Nitrogen1[w]

  2004cited by this paper
• Levine's Guide to Spss for Analysis of Variance

  2003cited by this paper
• Long-distance root-to-shoot transport of phytochelatins and cadmium in Arabidopsis

  2003cited by this paper
• TRANSPORTERS RESPONSIBLE FOR THE UPTAKE AND PARTITIONING OF NITROGENOUS SOLUTES.

  2001cited by this paper
• A novel method for growing Arabidopsis thaliana plants hydroponically

  2000cited by this paper
• Nitrate regulation of metabolism and growth.

  1999cited by this paper
• Drought-induced effects on nitrate reductase activity and mRNA and on the coordination of nitrogen and carbon metabolism in maize leaves

  1998cited by this paper
• The AXR1 and AUX1 genes of Arabidopsis function in separate auxin-response pathways.

  1995cited by this paper
• The partitioning of nitrate assimilation between root and shoot of higher plants

  1986cited by this paper
• Nitrate assimilation and translocation by higher plants: Comparative physiology and ecological consequences

  1985cited by this paper

• Ramie Outperforms Golden Buckwheat in Cd-Contaminated Farmland Phytoremediation in Southern China: Core Microbes, Plant Traits and Soil Properties

  2026cites this paper
• Integrative physio-biochemistry and transcriptomic insights into nitrogen-calcium interactions in pakchoi (Brassica chinensis L.) growth

  2026cites this paper
• Multi-omics analysis revealed adaptation mechanisms in roots of different nitrogen-efficiency peanut genotypes under low-nitrogen stress

  2026cites this paper
• Trichoderma asperellum benefits nitrogen acquisition and K+/Na+ homeostasis in wolfberry under salt stress via activation of root plasma membrane H+-ATPase.

  2025cites this paper
• Impacts of nitrogen fertilization and planting date on the physiology and yield of purple sweet potato at the extreme Northern edge of cultivation

  2025cites this paper
• Microplastic effects on soil nitrogen storage, nitrogen emissions, and ammonia volatilization in relation to soil health and crop productivity: mechanism and future consideration

  2025cites this paper
• Nitrogen Use Efficiency in Agriculture: Integrating Biotechnology, Microbiology, and Novel Delivery Systems for Sustainable Agriculture

  2025cites this paper
• Shining light on Arabidopsis regulatory networks integrating nitrogen use and photosynthesis

  2025cites this paper
• Tryptophan regulates sorghum root growth and enhances low nitrogen tolerance.

  2024cites this paper
• Effect of Transplanting Time and Nitrogen–Potassium Ratio on Yield, Growth, and Quality of Cauliflower Landrace Gigante di Napoli in Southern Italy

  2024cites this paper
• Green manuring improves soil quality, grain yield, and grain anthocyanin content in colored rice cultivated in tropical regions

  2024cites this paper
• Pearl Millet Seed Surface Modification and Improved Germination by Non-thermal Plasma Discharge: Understanding the Role of Reactive Species

  2024cites this paper
• Nitrogen availability in soil controls uptake of different nitrogen forms by plants

  2024cites this paper
• Synthesis and Evaluation of Starch-Grafted-Poly[(Acrylic Acid)-Co-Acrylamide] Based Nanoclay Polymer Composite Fertilizers for Slow Release of Nitrogen in Soil

  2024cites this paper
• Physiological changes underlying increased photosynthetic-nitrogen use efficiency in response to low-nitrogen conditions in Brassica napus L

  2024cites this paper
• The dominant mechanisms of nutrient cycling in high-dam reservoirs: retention, transport or transformation?

  2024cites this paper
• Establishment of a Comprehensive Evaluation System for Low Nitrogen and Screening of Nitrogen-Efficient Germplasm in Peanut

  2024cites this paper
• Elevated ambient CO2 promotes auxin-modulated NO3- uptake and growth of Brassica napus L. under low nitrogen availability

  2024cites this paper
• The response of roots and rhizosphere environment to integrative cultivation practices in paddy rice

  2023cites this paper
• The Mechanisms Underlying Physiological and Molecular Responses to Waterlogging in Flax

  2023influential citation
• Research Progress in Crop Root Biology and Nitrogen Uptake and Use, with Emphasis on Cereal Crops

  2023cites this paper
• CO2 enhances low-nitrogen adaption by promoting amino acid metabolism in Brassica napus.

  2023cites this paper
• Comparation Test on High-Quality Flax Resources for Oil and Fiber Production in Kazakhstan

  2023influential citation
• Soybean breeding in southwestern China improved P and N utilization efficiencies by increasing phosphorus and nitrogen partitioning to pods

  2023cites this paper
• CHLORIDE CHANNEL-b mediates vacuolar nitrate efflux to improve low nitrogen adaptation in Arabidopsis.

  2023cites this paper
• Varietal responses of root characteristics to low nitrogen application explain the differing nitrogen uptake and grain yield in two rice varieties

  2023cites this paper
• Partial substitution of chemical fertilizer by Trichoderma biofertilizer improved nitrogen use efficiency in wolfberry (Lycium chinense) in coastal saline land

  2023cites this paper
• Tuning excited-state electronic structure in tungsten oxide for enhanced nitrogen photooxidation as fertilizer

  2023cites this paper
• Comparative Assessment of Lignan, Tocopherol, Tocotrienol and Carotenoids in 40 Selected Varieties of Flaxseed (Linum usitatissimum L.)

  2023cites this paper
• Arbuscular Mycorrhizal Fungi Adjusts Root Architecture to Promote Leaf Nitrogen Accumulation and Reduce Leaf Carbon–Nitrogen Ratio of Mulberry Seedlings

  2023cites this paper
• Nitrogen–potassium balance improves leaf photosynthetic capacity by regulating leaf nitrogen allocation in apple

  2023cites this paper
• Modification of plasma membrane H+-ATPase in Masson pine (Pinus massoniana Lamb.) seedling roots adapting to acid deposition.

  2022cites this paper
• Improvements in grain yield and nutrient utilization efficiency of japonica inbred rice released since the 1980s in eastern China

  2022cites this paper
• Physiological Responses of Cigar Tobacco Crop to Nitrogen Deficiency and Genome-Wide Characterization of the NtNPF Family Genes

  2022influential citation
• Low Nitrogen Stress Stimulated Nitrate Uptake Rate Modulated by Auxin in Brassica napus L.

  2022cites this paper
• Nodule Formation and Nitrogen Use Efficiency Are Important for Soybean to Adapt to Water and P Deficit Conditions

  2022cites this paper
• PDF1.5 Enhances Adaptation to Low Nitrogen Levels and Cadmium Stress

  2021cites this paper
• Reduced nitrogen application rate with dense planting improves rice grain yield and nitrogen use efficiency: A case study in east China

  2021cites this paper
• Transcriptome Analysis Reveals Different Responsive Patterns to Nitrogen Deficiency in Two Wheat Near-Isogenic Lines Contrasting for Nitrogen Use Efficiency

  2021cites this paper
• Nitrogen stress inhibits root growth by regulating cell wall and hormone changes in cotton ( Gossypium hirsutum L.)

  2021cites this paper
• Physiological Characteristics and Transcriptomic Dissection in Two Root Segments with Contrasting Net Fluxes of Ammonium and Nitrate of Poplar Under Low Nitrogen Availability.

  2021cites this paper
• Nitrogen modulates cotton root morphology by affecting abscisic acid (ABA) and salicylic acid (SA) content

  2020cites this paper
• Genome-Wide Differential DNA Methylation and miRNA Expression Profiling Reveals Epigenetic Regulatory Mechanisms Underlying Nitrogen-Limitation-Triggered Adaptation and Use Efficiency Enhancement in Allotetraploid Rapeseed

  2020cites this paper
• Effect of dielectric barrier discharge cold plasma on pea seed growth.

  2019cites this paper
• Nodule Formation and Nitrogen Use Efficiency Are Important for Soybean to Adapt to Water and P Deficit Conditions

  year unknowncites this paper