Microsatellite DNA Polymorphism and Heterozygosity Among Field and Laboratory Populations of Anopheles gambiae s.s. (Diptera: Culicidae)

Abstract We compared microsatellite polymorphism at nine loci located on chromosome 3 among two colonies and a field population of Anopheles gambiae sensu stricto Giles mosquitoes. Numbers of microsatellite alleles observed at each locus and mean heterozygosities were drastically reduced among laboratory colonies. Genetic analysis of the field population used in this study revealed an unprecedented frequency of rare alleles (<0.05). In contrast, colony samples revealed large numbers of alleles with frequencies >0.50. Partitioning of field data to assess the impact of rare alleles, null alleles, and sample size on estimates of mean heterozygosity revealed the plasticity of this measurement and suggests that heterozygosity may be reliably estimated from relatively small collections using microsatellites.

• Publication year

  2001

• Venue

  Journal of medical entomology

• Publication date

  2001-03-01

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1603/0022-2585-38.2.336PMID 11296845
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Microsatellite evolution: polarity of substitutions within repeats and neutrality of flanking sequences

  1999cited by this paper
• Microsatellite markers and genotyping procedures for Anopheles gambiae.

  1999cited by this paper
• Estimates of gene flow among Anopheles maculatus populations in Thailand using microsatellite analysis.

  1999cited by this paper
• Analysis of genetic variability in Anopheles arabiensis and Anopheles gambiae using microsatellite loci

  1999cited by this paper
• The use of microsatellites to study gene flow in natural populations of Anopheles malaria vectors in Africa: potential and pitfalls.

  1998cited by this paper
• Complexities in the genetic structure of Anopheles gambiae populations in west Africa as revealed by microsatellite DNA analysis.

  1998cited by this paper
• Microgeographic genetic differentiation of Anopheles gambiae mosquitoes from Asembo Bay, western Kenya: a comparison with Kilifi in coastal Kenya.

  1998cited by this paper
• Patterns of genetic variability in colonized strains of Lutzomyia longipalpis (Diptera: Psychodidae) and its consequences.

  1997cited by this paper
• Molecular identification of sympatric chromosomal forms of Anopheles gambiae and further evidence of their reproductive isolation

  1997cited by this paper
• Microgeographic structure of Anopheles gambiae in western Kenya based on mtDNA and microsatellite loci

  1997influential reference
• Arlequin ver.1.1:a software for population genetic data analysis.

  1997cited by this paper
• A review of the use of ribosomal DNA (rDNA) to differentiate among cryptic Anopheles species

  1996cited by this paper
• Genetic differentiation of Anopheles gambiae populations from East and West Africa: comparison of microsatellite and allozyme loci

  1996influential reference
• An integrated genetic map of the African human malaria vector mosquito, Anopheles gambiae.

  1996influential reference
• Microsatellite DNA and isozyme variability in a West African population of Anopheles gambiae

  1995cited by this paper
• Unexpected Genetic Consequences of Colonization and Inbreeding: Allozyme Tracking in Culicidae (Diptera)

  1994cited by this paper
• Polymorphisms detected by random PCR distinguish between different chromosomal forms of Anopheles gambiae.

  1994cited by this paper
• A detailed genetic map for the X chromosome of the malaria vector, Anopheles gambiae.

  1993cited by this paper
• Identification of single specimens of the Anopheles gambiae complex by the polymerase chain reaction.

  1993cited by this paper
• Methods for the preservation of insects for DNA studies

  1993influential reference
• Use of the polymerase chain reaction to identify mosquito species of the Anopheles gambiae complex

  1990cited by this paper
• Heterozygosity in inbred strains of the tree-hole mosquitoAedes triseriatus

  1987cited by this paper
• Chromosomal inversion intergradation and incipient speciation in Anopheles gambiae

  1985influential reference
• Chromosomal differentiation and adaptation to human environments in the Anopheles gambiae complex.

  1979cited by this paper
• A biochemical key to adult members of the Anopheles gambiae group of species (Diptera: Culicidae).

  1979cited by this paper
• Enzyme variation in the Anopheles gambiae Giles group of species (Diptera: Culicidae)

  1978cited by this paper
• [THE ANOPHELES GAMBIAE COMPLEX IN WEST AFRICA].

  1964cited by this paper

• Defining the genetics of the widely used G3 strain of the mosquito, Anopheles gambiae

  2025influential citation
• Evidence for Significant Skew and Low Heritability of Competitive Male Mating Success in the Yellow Fever Mosquito Aedes aegypti

  2024cites this paper
• Culex tarsalis Is a Competent Host of the Insect-Specific Alphavirus Eilat Virus (EILV)

  2023cites this paper
• Application of Microsatellite Markers for Genetic Diversity Analysis of Indonesian Local Cattle

  2022cites this paper
• Flight Capability and the Low Temperature Threshold of a Chinese Field Population of the Fall Armyworm Spodoptera frugiperda

  2022cites this paper
• Prevalence of Plasmodium spp. in Anopheles mosquitoes in Thailand: a systematic review and meta-analysis

  2022cites this paper
• Special Collection: Genetic Control of Mosquitoes Malaria-Resistant Mosquitoes (Diptera: Culicidae); The Principle is Proven, But Will the Effectors Be Effective?

  2021cites this paper
• Phylogenetic Complexity of Morphologically Identified Anopheles squamosus in Southern Zambia

  2021cites this paper
• Special Collection: Genetic Control of Mosquitoes Sterile Insect Technique: Lessons From the Past

  2021cites this paper
• Phenotypic traits of individuals in a long-term colony of Anopheles (Nyssorhynchus) aquasalis (Diptera: Culicidae) show variable susceptibility to Plasmodium and suggest cryptic speciation.

  2021cites this paper
• Creating outbred and inbred populations in haplodiploids to measure adaptive responses in the laboratory

  2020cites this paper
• First development and characterization of 27 novel microsatellite markers in the dobsonfly Neoneuromus ignobilis (Megaloptera: Corydalidae) at genome-scale level

  2020cites this paper
• Genetic diversity of Anopheles coustani in high malaria transmission foci in southern and central Africa

  2020cites this paper
• Genetic Diversity of Anopheles coustani (Diptera: Culicidae) in Malaria Transmission Foci in Southern and Central Africa

  2020cites this paper
• Insecticide resistance, fitness and susceptibility to Zika infection of an interbred Aedes aegypti population from Rio de Janeiro, Brazil

  2020cites this paper
• First Observation of Experimental Plasmodium vivax Infection of Three Malaria Vectors from the Brazilian Amazon

  2020cites this paper
• Abundance of conserved CRISPR-Cas9 target sites within the highly polymorphic genomes of Anopheles and Aedes mosquitoes

  2020cites this paper
• Feasibility of feeding Aedes aegypti mosquitoes on dengue virus-infected human volunteers for vector competence studies in Iquitos, Peru

  2019cites this paper
• Acceptability of Aedes aegypti blood feeding on dengue virus-infected human volunteers for vector competence studies in Iquitos, Peru

  2019cites this paper
• Genetic Diversity and DNA Markers in Fish

  2019cites this paper
• Susceptibility of wild and colonized Anopheles stephensi to Plasmodium vivax infection

  2018cites this paper
• Reproducibility and relevance in insect-arbovirus infection studies

  2018cites this paper
• Selection and characterization of a new, non-melanising line of Anopheles gambiae s.s. refractory to Plasmodium falciparum

  2018influential citation
• Characterization of a local genetic sexing strain as well as a wild population of anopheles arabiensis from KwaZulu Natal, South Africa

  2017cites this paper
• Genetic diversity in a commercial black soldier fly, Hermetia illucens (Diptera: Stratiomydiae), population

  2017cites this paper
• Dynamics of Plasmodium vivax sporogony in wild Anopheles stephensi in a malaria-endemic region of Western India

  2017cites this paper
• Effects of malaria infection on mosquito olfaction and behavior: extrapolating data to the field.

  2017cites this paper
• Usefulness and accuracy of MALDI‐TOF mass spectrometry as a supplementary tool to identify mosquito vector species and to invest in development of international database

  2017cites this paper
• Characterisation of Species and Diversity of Anopheles gambiae Keele Colony

  2016influential citation
• Multi-locus genotyping reveals absence of genetic structure in field populations of the brown ear tick (Rhipicephalus appendiculatus) in Kenya.

  2016cites this paper
• Laboratory rearing of Anopheles arabiensis: impact on genetic variability and implications for Sterile Insect Technique (SIT) based mosquito control in northern Sudan

  2016cites this paper
• Application of a dense genetic map for assessment of genomic responses to selection and inbreeding in Heliothis virescens

  2016cites this paper
• Swarming and mating activity of Anopheles gambiae mosquitoes in semi‐field enclosures

  2016cites this paper
• Feeding Patterns and Xenomonitoring of Trypanosomes among Tsetse Flies around the Gashaka-Gumti National Park in Nigeria

  2016cites this paper
• Spatio-temporal heterogeneity of malaria vectors in northern Zambia: implications for vector control

  2016cites this paper
• Colonization of malaria vectors under semi-field conditions as a strategy for maintaining genetic and phenotypic similarity with wild populations

  2015cites this paper
• Limited genomic divergence between intraspecific forms of Culex pipiens under different ecological pressures

  2015cites this paper
• Comparison of genetic diversity and population structure between two Schistosoma japonicum isolates—the field and the laboratory

  2015cites this paper
• An overview of malaria transmission from the perspective of Amazon Anopheles vectors

  2015cites this paper
• Host-seeking behaviors of mosquitoes experimentally infected with sympatric field isolates of the human malaria parasite Plasmodium falciparum: no evidence for host manipulation

  2015cites this paper
• The genetics and ecology of male reproductive investments in the malaria mosquito Anopheles gambiae s.s

  2015cites this paper
• Changes in Genetic Diversity from Field to Laboratory During Colonization of Anopheles darlingi Root (Diptera: Culicidae).

  2015cites this paper
• Genomic study of Anophe les (Nyssorhynchus) aquasalis, Curry 1932. A Neotropical human malaria vector

  2015cites this paper
• The effect of environmental and metabolic stress on the expression of insecticide resistance phenotype and longevity in the Southern African malaria vectors anopheles arabiensis and anopheles funestus

  2015cites this paper
• Differential effects of inbreeding and selection on male reproductive phenotype associated with the colonization and laboratory maintenance of Anopheles gambiae

  2014cites this paper
• Infection of Laboratory-Colonized Anopheles darlingi Mosquitoes by Plasmodium vivax

  2014cites this paper
• Invasion du nématode du pin Bursaphelenchus xylophilus

  2014cites this paper
• Local adaptation to temperature and the implications for vector-borne diseases.

  2014cites this paper
• Effects of the kdr resistance mutation on the susceptibility of wild Anopheles gambiae populations to Plasmodium falciparum: a hindrance for vector control

  2014cites this paper
• Genome-Wide Patterns of Polymorphism in an Inbred Line of the African Malaria Mosquito Anopheles gambiae

  2014cites this paper
• OF EST MICROSATELLITES AND ANALYSIS OF POPULATION GENETIC STRUCTURE IN STRIPED SNAKEHEAD FISH

  2014cites this paper
• Equivalent susceptibility of Anopheles gambiae M and S molecular forms and Anopheles arabiensis to Plasmodium falciparum infection in Burkina Faso

  2013cites this paper
• Transcriptomic Profiling of Diverse Aedes aegypti Strains Reveals Increased Basal-level Immune Activation in Dengue Virus-refractory Populations and Identifies Novel Virus-vector Molecular Interactions

  2013cites this paper
• An investigation into the fitness and mating competitiveness of laboratory and transgenic strains of Anopheles gambiae sensu stricto

  2013cites this paper
• Phenotypic trait changes in laboratory – reared colonies of the maize herbivore, Diabrotica virgifera virgifera

  2013cites this paper
• Genetic and Environmental Factors Associated with Laboratory Rearing Affect Survival and Assortative Mating but Not Overall Mating Success in Anopheles gambiae Sensu Stricto

  2013cites this paper
• A Simple Chelex Protocol for DNA Extraction from Anopheles spp.

  2013cites this paper
• Efficacy of the D-Design Ifakara Tent Trap for Sampling Malaria Vectors in An Area of Mass Long Lasting Insecticidal Bed Nets Use

  2012cites this paper
• Gene flow-dependent genomic divergence between Anopheles gambiae M and S forms.

  2012cites this paper
• Micro- and minisatellite-expressed sequence tag (EST) markers discriminate between populations of Rhipicephalus appendiculatus.

  2012cites this paper
• Anopheles gambiae pathogen susceptibility: the intersection of genetics, immunity and ecology.

  2012cites this paper
• Genetic Structure of Tribolium castaneum (Coleoptera: Tenebrionidae) Populations in Mills

  2012cites this paper
• Plasmodium falciparum Produce Lower Infection Intensities in Local versus Foreign Anopheles gambiae Populations

  2012cites this paper
• ESTRUTURA POPULACIONAL DE Anopheles darlingi EM DIFERENTES LOCALIDADES DE RONDÔNIA AO LONGO DO RIO MADEIRA ATRAVÉS DA GENOTIPAGEM DE MICROSSATÉLITES

  2011cites this paper
• Microsatellite-based characterization of Southern African domestic pigs ( Sus scrofa domestica )

  2010cites this paper
• Ecological and evolutionary determinants of anopheline host species choice and its implications for malaria transmission

  2010cites this paper
• Behavioural, ecological, and genetic determinants of mating and gene flow in African malaria mosquitoes

  2010influential citation
• Genetic structure of Anopheles (Nyssorhynchus) marajoara (Diptera: Culicidae) in Colombia.

  2010cites this paper
• VARIASI GENETIK SUKU BATAK YANG TINGGAL DI KOTA DENPASAR DAN KABUPATEN BADUNG BERDASARKAN TIGA LOKUS MIKROSATELIT DNA AUTOSOM

  2010cites this paper
• Genome‐wide analysis of transcriptomic divergence between laboratory colony and field Anopheles gambiae mosquitoes of the M and S molecular forms

  2010cites this paper
• Authentication scheme for routine verification of genetically similar laboratory colonies: a trial with Anopheles gambiae

  2009cites this paper
• Shifting priorities in vector biology to improve control of vector‐borne disease

  2009cites this paper
• Levantamento de sarcofagídeos(Diptera) do Brasil incluindo a caracterização molecular de Peckia (Pattonella) intermutans (Walker)

  2009cites this paper
• Sialome Individuality Between Aedes aegypti Colonies

  2009cites this paper
• Colonisation and mass rearing: learning from others

  2009cites this paper
• Establishment of a large semi-field system for experimental study of African malaria vector ecology and control in Tanzania

  2008cites this paper
• Differences in mate acceptance and host plant recognition between wild and laboratory‐reared Busseola fusca (Fuller)

  2008cites this paper
• Local Adaptation and Vector-Mediated Population Structure in Plasmodium vivax Malaria

  2008cites this paper
• Applications of mosquito ecology for successful insect transgenesis-based disease prevention programs.

  2008cites this paper
• Association of A/T rich microsatellites with responses to artificial selection for larval developmental duration in the silkworm Bombyx mori.

  2008cites this paper
• Isolation and characterization of polymorphic microsatellite loci for the horn fly, Haematobia irritans (L.) (Diptera: Muscidae)

  2008cites this paper
• Nature or nurture in mosquito resistance to malaria?

  2007cites this paper
• A major genetic locus controlling natural Plasmodium falciparum infection is shared by East and West African Anopheles gambiae

  2007cites this paper
• Genetic impoverishment in laboratory cultures of the test organism Chironomus riparius

  2007cites this paper
• Consequences of inbreeding and reduced genetic variation on tolerance to cadmium stress in the midge Chironomus riparius.

  2007cites this paper
• Genetic Differences Between Culex pipiens f. molestus and Culex pipiens pipiens (Diptera: Culicidae) in New York

  2007cites this paper
• Coadaptation and malaria control.

  2007cites this paper
• Genetic Diversity in Laboratory Colonies of Western Corn Rootworm (Coleoptera: Chrysomelidae), Including a Nondiapause Colony

  2007cites this paper
• Susceptibility of Anopheles gambiae and Anopheles stephensi to tropical isolates of Plasmodium falciparum

  2007cites this paper
• Genetic differentiation between the BAMAKO and SAVANNA chromosomal forms of Anopheles gambiae as indicated by amplified fragment length polymorphism analysis.

  2006cites this paper
• Gene flowanalysis of anopheles arabiensis (Diptera:culicidae) populations in southern africa using microsatellite DNA markers

  2006cites this paper
• Anopheles infection responses; laboratory models versus field malaria transmission systems.

  2005cites this paper
• Identification of mammalian blood meals in mosquitoes by a multiplexed polymerase chain reaction targeting cytochrome B.

  2005cites this paper
• Mitochondrial DNA divergence between wild and laboratory populations of Anopheles albimanus Wiedemann (Diptera: Culicidae)

  2005cites this paper
• Population genetic structure of Anopheles gambiae mosquitoes on Lake Victoria islands, west Kenya

  2004cites this paper
• A comparison of microsatellite polymorphism and heterozygosity among field and laboratory populations of Schistosoma mansoni.

  2004cites this paper
• Molecular differentiation of colonized human malaria vectors by 28S ribosomal DNA polymorphisms.

  2004cites this paper
• Animal Domestication and Behavior

  2003cites this paper
• Genetic Loci Affecting Resistance to Human Malaria Parasites in a West African Mosquito Vector Population

  2002cites this paper
• Ticks and Tick-borne Diseases

  year unknowncites this paper