Getting the big beast to work--systems biotechnology of Bacillus megaterium for novel high-value proteins.

No abstract is available for this paper.

• Publication year

  2013

• Venue

  Journal of Biotechnology

• Publication date

  2013-01-20

• Fields of study

  Biology, Medicine, Engineering, Environmental Science

• Identifiers
  DOI 10.1016/j.jbiotec.2012.06.018PMID 22750448
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Debottlenecking recombinant protein production in Bacillus megaterium under large‐scale conditions—targeted precursor feeding designed from metabolomics

  2012cited by this paper
• Viability and membrane potential analysis of Bacillus megaterium cells by impedance flow cytometry

  2012cited by this paper
• Systems and synthetic metabolic engineering for amino acid production - the heartbeat of industrial strain development.

  2012cited by this paper
• Influence of the hydromechanical stress and temperature on growth and antibody fragment production with Bacillus megaterium

  2011cited by this paper
• Erratum to: Transcriptome profiling of degU expression reveals unexpected regulatory patterns in Bacillus megaterium and discloses new targets for optimizing expression

  2011cited by this paper
• Influence of fructose and oxygen gradients on fed‐batch recombinant protein production using Bacillus megaterium

  2011cited by this paper
• Complete Genome Sequence of the Industrial Strain Bacillus megaterium WSH-002

  2011cited by this paper
• Antibody production in Bacillus megaterium: strategies and physiological implications of scaling from microtiter plates to industrial bioreactors.

  2011cited by this paper
• Transcriptome profiling of degU expression reveals unexpected regulatory patterns in Bacillus megaterium and discloses new targets for optimizing expression

  2011cited by this paper
• Genome Sequences of the Biotechnologically Important Bacillus megaterium Strains QM B1551 and DSM319

  2011cited by this paper
• Single cell analysis applied to antibody fragment production with Bacillus megaterium: development of advanced physiology and bioprocess state estimation tools

  2011cited by this paper
• Opportunities for yeast metabolic engineering: Lessons from synthetic biology

  2011cited by this paper
• Functional analysis of the response regulator DegU in Bacillus megaterium DSM319 and comparative secretome analysis of degSU mutants

  2011cited by this paper
• Systems biology of recombinant protein production using Bacillus megaterium.

  2011cited by this paper
• Application of Escherichia coli phage K1E DNA-dependent RNA polymerase for in vitro RNA synthesis and in vivo protein production in Bacillus megaterium

  2010cited by this paper
• Optimization of antibody fragment production in Bacillus megaterium: the role of metal ions on protein secretion.

  2010cited by this paper
• An improved transconjugation protocol for Bacillus megaterium facilitating a direct genetic knockout

  2010cited by this paper
• Advanced tool for characterization of microbial cultures by combining cytomics and proteomics

  2010cited by this paper
• A short story about a big magic bug

  2010cited by this paper
• Metabolic fluxes and beyond—systems biology understanding and engineering of microbial metabolism

  2010cited by this paper
• Metabolic engineering of Escherichia coli for the production of polylactic acid and its copolymers

  2010cited by this paper
• High-Yield Intra- and Extracellular Protein Production Using Bacillus megaterium

  2010cited by this paper
• Metabolic engineering of cobalamin (vitamin B12) production in Bacillus megaterium

  2009cited by this paper
• Pectinase production by Bacillus subtilis and its potential application in biopreparation of cotton and micropoly fabric

  2009cited by this paper
• Metabolic engineering of microorganisms: general strategies and drug production.

  2009cited by this paper
• A T7 RNA polymerase-dependent gene expression system for Bacillus megaterium

  2009cited by this paper
• Inexpensive fed-batch cultivation for high poly(3-hydroxybutyrate) production by a new isolate of Bacillus megaterium.

  2009cited by this paper
• Metabolic Engineering of the Tricarboxylic Acid Cycle for Improved Lysine Production by Corynebacterium glutamicum

  2009cited by this paper
• Physiological response of Corynebacterium glutamicum to oxidative stress induced by deletion of the transcriptional repressor McbR.

  2008cited by this paper
• Recent Advances in High Cell Density Cultivation for Production of Recombinant Protein

  2008cited by this paper
• Metabolic engineering of Escherichia coli for the production of malic acid

  2008cited by this paper
• Sampling for metabolome analysis of microorganisms.

  2007cited by this paper
• Bacillus megaterium—from simple soil bacterium to industrial protein production host

  2007cited by this paper
• Production of single chain Fab (scFab) fragments in Bacillus megaterium

  2007cited by this paper
• A sucrose-inducible promoter system for the intra- and extracellular protein production in Bacillus megaterium.

  2007cited by this paper
• Response of fluxome and metabolome to temperature-induced recombinant protein synthesis in Escherichia coli.

  2007cited by this paper
• Codon optimized Thermobifida fusca hydrolase secreted by Bacillus megaterium

  2007cited by this paper
• Effect of different carbon sources on central metabolic fluxes and the recombinant production of a hydrolase from Thermobifida fusca in Bacillus megaterium.

  2007cited by this paper
• Export, purification, and activities of affinity tagged Lactobacillus reuteri levansucrase produced by Bacillus megaterium

  2007cited by this paper
• The penicillin G acylase production by B. megaterium is amino acid consumption dependent

  2007cited by this paper
• Cloning and expression of keratinase gene in Bacillus megaterium and optimization of fermentation conditions for the production of keratinase by recombinant strain

  2007cited by this paper
• Plasmid system for the intracellular production and purification of affinity‐tagged proteins in Bacillus megaterium

  2007cited by this paper
• The penicillin G acylase production by B. megaterium is amino acid consumption dependent

  2007cited by this paper
• Proteomic characterization of transient expression and secretion of a stress-related metalloprotease in high cell density culture of Bacillus megaterium.

  2006cited by this paper
• Using a medium of free amino acids to produce penicillin g acylase in fed-batch cultivations of Bacillus megaterium ATCC 14945

  2006cited by this paper
• Comparative proteomic analysis of high cell density cultivations with two recombinant Bacillus megaterium strains for the production of a heterologous dextransucrase

  2006cited by this paper
• Comparative study on central metabolic fluxes of Bacillus megaterium strains in continuous culture using 13C labelled substrates

  2006cited by this paper
• A Bacillus megaterium Plasmid System for the Production, Export, and One-Step Purification of Affinity-Tagged Heterologous Levansucrase from Growth Medium

  2006cited by this paper
• Studies on lysine production by Bacillus megaterium

  2005cited by this paper
• Production and secretion of recombinant Leuconostoc mesenteroides dextransucrase DsrS in Bacillus megaterium.

  2005cited by this paper
• Coexpression of the type I signal peptidase gene sipM increases recombinant protein production and export in Bacillus megaterium MS941.

  2005cited by this paper
• Proteome analysis of a recombinant Bacillus megaterium strain during heterologous production of a glucosyltransferase

  2005cited by this paper
• Evidence for two recA genes mediating DNA repair in Bacillus megaterium.

  2005cited by this paper
• Strain development in Bacillus licheniformis: construction of biologically contained mutants deficient in sporulation and DNA repair.

  2005cited by this paper
• Physiological and technological aspects of large-scale heterologous-protein production with yeasts

  2004cited by this paper
• Pyruvate formation and suppression in recombinant Bacillus megaterium cultivation.

  2004cited by this paper
• Molecular cloning, structure, promoters and regulatory elements for transcription of the Bacillus megaterium encoded regulon for xylose utilization

  2004cited by this paper
• Molecular cloning, structure, promoters and regulatory elements for transcription of the Bacillus licheniformis encoded regulon for xylose utilization

  2004cited by this paper
• Overproduction and secretion of Bacillus circulans endo-β-1,3-1,4-glucanase gene (bglBC1) in B. subtilis and B. megaterium

  2003cited by this paper
• Applications of inulin and oligofructose in health and nutrition

  2002cited by this paper
• Nutritional aspects of short-chain fructooligosaccharides: natural occurrence, chemistry, physiology and health implications.

  2002cited by this paper
• Cloning, expression, and carbon catabolite repression of the bamM gene encoding β-amylase of Bacillus megaterium DSM319

  2001cited by this paper
• Study of different media for production of penicillin G acylase from Bacillus megaterium ATCC 14945

  2000cited by this paper
• Cloning of oxetanocin A biosynthetic and resistance genes that reside on a plasmid of Bacillus megaterium strain NK84-0128.

  1999cited by this paper
• Accurate flow cytometric membrane potential measurement in bacteria using diethyloxacarbocyanine and a ratiometric technique.

  1999cited by this paper
• Signal Peptide Peptidase- and ClpP-like Proteins ofBacillus subtilis Required for Efficient Translocation and Processing of Secretory Proteins*

  1999cited by this paper
• Molecular characterization of the operon comprising the spoIV gene of Bacillus megaterium DSM319 and generation of a deletion mutant.

  1998cited by this paper
• Production and characterization of xylanase from a beta-amylolytic strain of Bacillus megaterium.

  1997cited by this paper
• Clinical evaluation of carbocyclic oxetanocin G eyedrops in the treatment of herpes simplex corneal ulcers.

  1996cited by this paper
• Transformation of Bacillus megaterium by electroporation

  1995cited by this paper
• Inactivation of the major extracellular protease from Bacillus megaterium DSM319 by gene replacement

  1995cited by this paper
• Development of genetic engineering in Bacillus megaterium.

  1992cited by this paper
• Cloning, nucleotide sequences, and enzymatic properties of glucose dehydrogenase isozymes from Bacillus megaterium IAM1030

  1992cited by this paper
• Catabolite repression of the xyl operon in Bacillus megaterium

  1992cited by this paper
• A ring-enlarged oxetanocin A analogue as an inhibitor of HIV infectivity.

  1991cited by this paper
• Inducible high-level expression of heterologous genes in Bacillus megaterium using the regulatory elements of the xylose-utilization operon

  1991cited by this paper
• Biolistic transformation of a procaryote, Bacillus megaterium

  1991cited by this paper
• Efficient cloning in Bacillus megaterium: comparison to Bacillus subtilis and Escherichia coli cloning hosts.

  1990cited by this paper
• High cell density culture of E. coli in a fed-batch system with dissolved oxygen as substrate feed indicator

  1989cited by this paper
• Highly efficient expression of homologous and heterologous genes in Bacillus megaterium

  1989cited by this paper
• Biosensing devices for the semi-automated control of dehydrogenase substrates in fermentations

  1989cited by this paper
• Microbial amylolytic enzymes.

  1989cited by this paper
• Benefits of Bacillus megaterium Amylase in Dextrose Production

  1988cited by this paper
• Isolation and genetic characterizations of Bacillus megaterium cobalamin biosynthesis-deficient mutants

  1986cited by this paper
• Oxetanocin, a novel nucleoside from bacteria.

  1986cited by this paper
• Heterogeneous circular DNA elements in vegetative cultures of Bacillus megaterium.

  1969cited by this paper
• Microbial Cell Factories Production of Recombinant Antibody Fragments in Bacillus Megaterium

  year unknowncited by this paper
• Microbial Cell Factories High Yield Recombinant Penicillin G Amidase Production and Export into the Growth Medium Using Bacillus Megaterium

  year unknowncited by this paper
• Vergleichende Morphologie und Biologie der Pilze, Mycetozoen und Bacterien

  year unknowncited by this paper

• The role of Bacillus megaterium PEF-1 in stimulating the growth of organic cotton under environmental stress conditions

  2025cites this paper
• UV protection and insecticidal activity of microencapsulated Vip3Ag4 protein in Bacillus megaterium.

  2024cites this paper
• Insecticidal Activity of Microencapsulated Vip3Ag4 protein in Bacillus megaterium

  2023cites this paper
• Recovery and utilization of waste filtrate from industrial biological fermentation: Development and metabolite profile of the Bacillus cereus liquid bio-fertilizer.

  2023cites this paper
• Structural comparison of the cytochrome P450 enzymes CYP106A1 and CYP106A2 provides insight into their differences in steroid conversion

  2022cites this paper
• Construction and Application of a Plasmid-Based Signal Peptide Library for Improved Secretion of Recombinant Proteins with Priestia megaterium

  2022cites this paper
• Enhanced Glutamate Synthesis and Export by the Thermotolerant Emerging Industrial Workhorse Bacillus methanolicus in Response to High Osmolarity

  2021cites this paper
• Genomic analysis of a functional haloacid-degrading gene of Bacillus megaterium strain BHS1 isolated from Blue Lake (Mavi Gölü, Turkey)

  2021cites this paper
• Bacillus megaterium: amino acid producer and probiotic for farm animals (review)

  2020cites this paper
• Genomic Analysis of Bacillus megaterium NCT-2 Reveals Its Genetic Basis for the Bioremediation of Secondary Salinization Soil

  2020cites this paper
• Development and evolution of biocyanidation in metal recovery from solid waste: a review

  2020cites this paper
• Diversity and evolutionary dynamics of spore-coat proteins in spore-forming species of Bacillales

  2020cites this paper
• Metabolic Rearrangements Causing Elevated Proline and Polyhydroxybutyrate Accumulation During the Osmotic Adaptation Response of Bacillus megaterium

  2020cites this paper
• Drawing up a collaborative contract: Amino acid cross‐feeding between interspecies bacterial pairs

  2020influential citation
• High-yield C11-oxidation of hydrocortisone by establishment of an efficient whole-cell system in Bacillus megaterium.

  2019cites this paper
• Preparation of Biocomposite Microfibers Ready for Processing into Biologically Active Textile Fabrics for Bioremediation.

  2018cites this paper
• Isolation, Development, and Genomic Analysis of Bacillus megaterium SR7 for Growth and Metabolite Production Under Supercritical Carbon Dioxide

  2018cites this paper
• Bacillus megaterium: an endophytic bacteria from callus of Ilex paraguariensis with growth promotion activities.

  2018cites this paper
• Rapid acquisition and model-based analysis of cell-free transcription–translation reactions from nonmodel bacteria

  2018cites this paper
• General aspects of cytochrome P 450 monooxygenases Cytochrome

  2018cites this paper
• Cloning and characterization of a novel intracellular serine protease (IspK) from Bacillus megaterium with a potential additive for detergents.

  2017cites this paper
• Characterization of cytochrome P450 CYP109E1 from Bacillus megaterium as a novel vitamin D3 hydroxylase.

  2017cites this paper
• Engineering Bacillus megaterium for production of functional intracellular materials

  2017cites this paper
• Efficient Biotransformation of Astragaloside IV to Cycloastragenol by Bacillus sp. LG-502

  2017cites this paper
• Prototyping of Bacillus megaterium genetic elements through automated cell-free characterization and Bayesian modelling

  2016cites this paper
• Utilization of microorganisms in the solubilization of low-quality phosphorus raw material

  2016cites this paper
• Surveying and harnessing the genetic, (meta)genomic, and metabolic potential of the deep carbonated biosphere

  2016influential citation
• Expression of human CYP27A1 in B. megaterium for the efficient hydroxylation of cholesterol, vitamin D3 and 7-dehydrocholesterol.

  2016cites this paper
• Synthetic Quorum Sensing and Cell-Cell Communication in Gram-Positive Bacillus megaterium.

  2016cites this paper
• Draft Genome Sequence of Bacillus megaterium BHG1.1, a Strain Isolated from Bar-Headed Goose (Anser indicus) Feces on the Qinghai-Tibet Plateau

  2016cites this paper
• Bio-recovery of non-essential heavy metals by intra- and extracellular mechanisms in free-living microorganisms.

  2016cites this paper
• A Bacillus megaterium System for the Production of Recombinant Proteins and Protein Complexes.

  2016cites this paper
• PGPR Potentially Improve Growth of Tomato Plants in Salt-Stressed Environment

  2016cites this paper
• Complete Genome of Bacillus megaterium Podophage Pookie

  2015cites this paper
• Comparison of CYP106A1 and CYP106A2 from Bacillus megaterium – identification of a novel 11-oxidase activity

  2015cites this paper
• Impact of rare codons and the functional coproduction of rate-limiting tRNAs on recombinant protein production in Bacillus megaterium

  2015cites this paper
• Complete Genome Sequence of Bacillus megaterium Myophage Moonbeam

  2015cites this paper
• Complete Genome Sequence of Bacillus megaterium Podophage Pavlov

  2015cites this paper
• Complete Genome Sequence of Bacillus megaterium Myophage Mater

  2015cites this paper
• Steroid conversions with the CYP106A subfamily from Bacillus megaterium

  2015cites this paper
• Heterologous Expression and Characterization of Thermostable Levansucrase (BsSacB) from Bacillus subtilis BB03

  2015cites this paper
• Steroid conversions with the CYP106A subfamily from Bacillus megaterium

  2015cites this paper
• Role of PGPR to improve potential growth of tomato under saline condition: An overview

  2015cites this paper
• Système d'expression de protéines recombinées chez bacillus megaterium

  2015cites this paper
• Production of phosphate biofertilizers from bones by phosphate-solubilizing bacteria Bacillus megaterium

  2015cites this paper
• Recent Improvements in Production of Recombinant Proteins

  2014cites this paper
• Basic studies on the role of components of Bacillus megaterium as flotation biocollectors in sulphide mineral separation

  2014cites this paper
• Protein secretion biotechnology in Gram-positive bacteria with special emphasis on Streptomyces lividans.

  2014cites this paper
• Towards a cell factory for vitamin B12 production in Bacillus megaterium: bypassing of the cobalamin riboswitch control elements.

  2014cites this paper
• Bacillus megaterium — ein Produktionssystem für rekombinante Proteine

  2014influential citation
• Systems metabolic engineering of Escherichia coli for production of the antitumor drugs violacein and deoxyviolacein.

  2013cites this paper
• Recent Improvements in Production of Recombinant Pro

  2013cites this paper
• Biotechnological production of feed nucleotides by microbial strain improvement

  2013cites this paper
• High yield production of extracellular recombinant levansucrase by Bacillus megaterium

  2013cites this paper
• Peptide‐based communication system enables Escherichia coli to Bacillus megaterium interspecies signaling

  2013cites this paper