Biomimetic surface patterning for long-term transmembrane access

Here we present a planar patch clamp chip based on biomimetic cell membrane fusion. This architecture uses nanometer length-scale surface patterning to replicate the structure and function of membrane proteins, creating a gigaohm seal between the cell and a planar electrode array. The seal is generated passively during cell spreading, without the application of a vacuum to the cell surface. This interface can enable cell-attached and whole-cell recordings that are stable to 72 hours and generates no visible damage to the cell. The electrodes can be very small (<5 μm) and closely packed, offering a high density platform for cellular measurement.

• Publication year

  2016

• Venue

  Scientific Reports

• Publication date

  2016-08-31

• Fields of study

  Biology, Medicine, Materials Science, Engineering

• Identifiers
  DOI 10.1038/srep32485PMID 27577519PMCID 5006161
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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