Study and selection of in vivo protein interactions by coupling bimolecular fluorescence complementation and flow cytometry

No abstract is available for this paper.

• Publication year

  2007

• Venue

  Nature Protocols

• Publication date

  Unknown publication date

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1038/nprot.2007.496PMID 18193018
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Analysis of Lipolytic Protein Trafficking and Interactions in Adipocytes*

  2007cited by this paper
• Bimolecular fluorescence complementation analysis system for in vivo detection of protein–protein interaction in Saccharomyces cerevisiae

  2007cited by this paper
• Analysis of protein complexes using mass spectrometry

  2007cited by this paper
• UNC-1 regulates gap junctions important to locomotion in C. elegans.

  2007cited by this paper
• In vivo visualization of actin dynamics and actin interactions by BiFC

  2007cited by this paper
• Identification of nuclear dicing bodies containing proteins for microRNA biogenesis in living Arabidopsis plants.

  2007cited by this paper
• Detection of transient protein–protein interactions by bimolecular fluorescence complementation: The Abl‐SH3 case

  2007cited by this paper
• Use of Bimolecular Fluorescence Complementation To Study In Vivo Interactions between Cdc42p and Rdi1p of Saccharomyces cerevisiae

  2007cited by this paper
• Identifying off-target effects and hidden phenotypes of drugs in human cells

  2006cited by this paper
• Identification of new fluorescent protein fragments for bimolecular fluorescence complementation analysis under physiological conditions.

  2006cited by this paper
• Assessment of the integral membrane protein topology in living cells.

  2006cited by this paper
• An improved mRFP1 adds red to bimolecular fluorescence complementation

  2006cited by this paper
• Design and implementation of bimolecular fluorescence complementation (BiFC) assays for the visualization of protein interactions in living cells

  2006cited by this paper
• Combinatorial control of Arabidopsis proline dehydrogenase transcription by specific heterodimerisation of bZIP transcription factors

  2006cited by this paper
• Quality assurance for polychromatic flow cytometry

  2006cited by this paper
• Detecting protein-protein interactions with a green fluorescent protein fragment reassembly trap: scope and mechanism.

  2005cited by this paper
• Reassembled GFP: detecting protein-protein interactions and protein expression patterns.

  2005cited by this paper
• Use of bimolecular fluorescence complementation to demonstrate transcription factor interaction in nuclei of living cells from the filamentous fungus Acremonium chrysogenum

  2005cited by this paper
• Determination of tumor necrosis factor receptor-associated factor trimerization in living cells by CFP→YFP→mRFP FRET detected by flow cytometry

  2005cited by this paper
• Capturing protein interactions in the secretory pathway of living cells.

  2005cited by this paper
• Visualization of protein interactions in living plant cells using bimolecular fluorescence complementation.

  2004cited by this paper
• Single-Cell Microbiology: Tools, Technologies, and Applications

  2004cited by this paper
• Green fluorescent protein–propidium iodide (GFP‐PI) based assay for flow cytometric measurement of bacterial viability

  2004cited by this paper
• Ubiquitin-mediated fluorescence complementation reveals that Jun ubiquitinated by Itch/AIP4 is localized to lysosomes.

  2004cited by this paper
• Detecting protein-protein interactions with GFP-fragment reassembly

  2004cited by this paper
• Inhibition of Mist1 Homodimer Formation Induces Pancreatic Acinar-to-Ductal Metaplasia

  2004cited by this paper
• Using flow cytometry to quantify microbial heterogeneity.

  2003cited by this paper
• Simultaneous visualization of multiple protein interactions in living cells using multicolor fluorescence complementation analysis

  2003cited by this paper
• A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications

  2002cited by this paper
• Visualization of interactions among bZIP and Rel family proteins in living cells using bimolecular fluorescence complementation.

  2002cited by this paper
• Application of Green Fluorescent Protein

  2001cited by this paper
• Molecular Cloning: A Laboratory Manual

  2001cited by this paper
• Imaging biochemistry inside cells.

  2001cited by this paper
• LIGA Technologies and Applications

  2001cited by this paper
• Antiparallel Leucine Zipper-Directed Protein Reassembly: Application to the Green Fluorescent Protein

  2000cited by this paper
• Sorting of Bacteria

  1999cited by this paper
• Crystal structure of the abl-SH3 domain complexed with a designed high-affinity peptide ligand: implications for SH3-ligand interactions.

  1998cited by this paper
• The proto-oncogene product p120CBL and the adaptor proteins CRKL and c-CRK link c-ABL, p190BCR/ABL and p210BCR/ABL to the phosphatidylinositol-3' kinase pathway.

  1996cited by this paper
• Introduction of a loss-of-function point mutation from the SH3 region of the Caenorhabditis elegans sem-5 gene activates the transforming ability of c-abl in vivo and abolishes binding of proline-rich ligands in vitro.

  1995cited by this paper
• High efficiency transformation of Escherichia coli with plasmids.

  1990cited by this paper
• A Laboratory manual

  1989cited by this paper
• A novel genetic system to detect protein–protein interactions

  1989cited by this paper
• Molecular cloning : a laboratory manual. Cold Spring Harbor, NY

  1989cited by this paper
• Filamentous fusion phage: novel expression vectors that display cloned antigens on the virion surface.

  1985cited by this paper
• Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.

  1972cited by this paper
• Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

  1970cited by this paper
• Scope and Mechanism

  year unknowncited by this paper

• Self-Assembly of Nanogold Triplets on Trimeric Viral Proteins for Infectious Disease Diagnosis.

  2025cites this paper
• STN1 Shields CTC1 From TRIM32‐Mediated Ubiquitination to Prevent Cellular Aging

  2025cites this paper
• Improving the Synthesis Efficiency of Amino Acids by Analyzing the Key Sites of Intracellular Self-Assembly of Artificial Cellulosome

  2024cites this paper
• Transmembrane proteins in grape immunity: current knowledge and methodological advances

  2024cites this paper
• Arginine (315) is required for the PLIN2-CGI-58 interface and plays a functional role in regulating nascent LDs formation in bovine adipocytes.

  2024cites this paper
• Chemical and Biological Strategies for Profiling Protein‐Protein Interactions in Living Cells

  2023cites this paper
• Study of the Bcl-2 Interactome by BiFC Reveals Differences in the Activation Mechanism of Bax and Bak

  2023cites this paper
• Oxidized LDL but not angiotensin II induces cardiomyocyte hypertrophic responses through the interaction between LOX-1 and AT1 receptors.

  2021cites this paper
• Cardiolipin is required for membrane docking of mitochondrial ribosomes and protein synthesis

  2020cites this paper
• Gamma-glutamyl carboxylase mutations differentially affect the biological function of vitamin K-dependent proteins.

  2020cites this paper
• Simultaneous introduction of multiple biomacromolecules into plant cells using a cell-penetrating peptide nanocarrier.

  2020cites this paper
• Cyclin Y, a novel actin-binding protein, regulates spine plasticity through the cofilin-actin pathway.

  2020cites this paper
• Oxidized LDL but not angiotensin II induces the interaction between LOX-1 and AT1 receptors

  2020cites this paper
• Overexpression of zmm28 increases maize grain yield in the field

  2019cites this paper
• Live Cell Visualization of Multiple Protein-Protein Interactions with BiFC Rainbow.

  2018cites this paper
• NEDD4 controls spermatogonial stem cell homeostasis and stress response by regulating messenger ribonucleoprotein complexes

  2017cites this paper
• Evolutionary Alternatives on the Road to a New Specificity in a G Protein-Coupled Receptor

  2017cites this paper
• Quantitative analysis of protein interaction network dynamics in yeast

  2017cites this paper
• Expanding the optogenetic toolkit with a photocleavable protein and a near infrared pH indicator

  2017cites this paper
• Association of TrkA and APP Is Promoted by NGF and Reduced by Cell Death-Promoting Agents

  2017cites this paper
• Unraveling the molecular mechanisms underlying alpha-synuclein oligomerization and cytotoxicity

  2017cites this paper
• Cytorhabdovirus P protein suppresses RISC-mediated cleavage and RNA silencing amplification in planta.

  2016cites this paper
• Sensing of biomolecular interactions using fluorescence complementing systems in living cells.

  2016cites this paper
• Evolution of a G protein-coupled receptor response by mutations in regulatory network interactions

  2016cites this paper
• Development of a new cellular model for the visualization and study of GFAP oligomerization in living cells

  2016cites this paper
• The Elucidation of the Interactome of 16 Arabidopsis bZIP Factors Reveals Three Independent Functional Networks

  2015cites this paper
• Development of New Cellular Models for Step-by-Step Analysis of Astrogliogenic Pathways

  2015cites this paper
• Phenotypic Analysis of NCR1, the Yeast Niemann Pick Type-C Disease Gene

  2015cites this paper
• Application of intra-molecular fluorescence complementation in the topology examination of polytopic proteins in living cells.

  2015cites this paper
• ZSCAN4 and TRF1: A functionally indirect interaction in cancer cells independent of telomerase activity.

  2015cites this paper
• Protein fragment bimolecular fluorescence complementation analyses for the in vivo study of protein-protein interactions and cellular protein complex localizations.

  2014influential citation
• New insights into the functional organization of xyloglucan biosynthetic enzymes and their catalytic mechanism

  2014cites this paper
• Biophysical Detection of Diversity and Bias in GPCR Function

  2014cites this paper
• Characterization of the interactions of baculovirus transcription and replication regulators by bimolecular fluorescence complementation

  2014cites this paper
• Optimisation of expression of recombinant puroindolines and analysis of protein-protein interactions in planta

  2014cites this paper
• Fluorescent dye ProteoStat to detect and discriminate intracellular amyloid-like aggregates in Escherichia coli.

  2014cites this paper
• Bimolecular Fluorescence Complementation (BiFC) Assay for Direct Visualization of Protein-Protein Interaction in vivo.

  2013cites this paper
• Plant protein interactomes.

  2013cites this paper
• Characterization of protein-protein interactions involved in auxin signaling pathway in tomato

  2013cites this paper
• Visualization and Quantification of APP Intracellular Domain-Mediated Nuclear Signaling by Bimolecular Fluorescence Complementation

  2013cites this paper
• Identification of Protein Interactions Involved in Cellular Signaling

  2013cites this paper
• Interactome mapping for analysis of complex phenotypes: Insights from benchmarking binary interaction assays

  2012cites this paper
• Bimolecular fluorescence complementation and interaction of various Arabidopsis major intrinsic proteins expressed in yeast.

  2012cites this paper
• Networks of ProteinProtein Interactions: From Uncertainty to Molecular Details

  2012cites this paper
• Survivin inhibition by an interacting recombinant peptide, derived from the human ferritin heavy chain, impedes tumor cell growth

  2012cites this paper
• Diversity in Genetic In Vivo Methods for Protein-Protein Interaction Studies: from the Yeast Two-Hybrid System to the Mammalian Split-Luciferase System

  2012cites this paper
• Thioflavin-S staining coupled to flow cytometry. A screening tool to detect in vivo protein aggregation.

  2012cites this paper
• Xyloglucan Xylosyltransferases XXT1, XXT2, and XXT5 and the Glucan Synthase CSLC4 Form Golgi-Localized Multiprotein Complexes1[W][OA]

  2012cites this paper
• Xyloglucan Xylosyltransferases XXT 1 , XXT 2 , and XXT 5 and the Glucan Synthase CSLC 4 Form Golgi-Localized Multiprotein Complexes 1 [ W ] [ OA ]

  2012influential citation
• Calcyon Forms a Novel Ternary Complex with Dopamine D1 Receptor through PSD-95 Protein and Plays a Role in Dopamine Receptor Internalization*

  2012cites this paper
• High Throughput Affinity Purification and Mass Spectrometry to Determine Protein Complex Interactions

  2012cites this paper
• Investigation into the stability of WEE1 kinase in plants

  2012cites this paper
• Dopamine Receptor Internalization PSD-95 Protein and Plays a Role in Receptor through 1 with Dopamine D Calcyon Forms a Novel Ternary Complex Cell Biology:

  2012cites this paper
• Genetically encodable fluorescent biosensors for tracking signaling dynamics in living cells.

  2011cites this paper
• Sub-cellular distribution of UNC-104(KIF1A) upon binding to adaptors as UNC-16(JIP3), DNC-1(DCTN1/Glued) and SYD-2(Liprin-α) in C. elegans neurons.

  2011cites this paper
• Imaging-based approaches to understanding g protein-coupled receptor signalling complexes.

  2011cites this paper
• Guide to red fluorescent proteins and biosensors for flow cytometry.

  2011cites this paper
• Fluorescent protein complementation assays: new tools to study G protein-coupled receptor oligomerization and GPCR-mediated signaling.

  2011cites this paper
• Bimolecular fluorescence complementation: illuminating cellular protein interactions.

  2011cites this paper
• Novel screening system for protein-protein interactions by bimolecular fluorescence complementation in Saccharomyces cerevisiae.

  2011cites this paper
• LEC–BiFC: a new method for rapid assay of protein interaction

  2011cites this paper
• Identification of novel Lis1 protein interaction partners: Investigations towards cellular Lis1 functions

  2010cites this paper
• Interactomics: Bimolecular Fluorescence Complementation (BiFC)

  2010cites this paper
• Zooming into protein oligomerization in neurodegeneration using BiFC.

  2010cites this paper
• A novel far-red bimolecular fluorescence complementation system that allows for efficient visualization of protein interactions under physiological conditions.

  2009cites this paper
• Detecting and interfering protein interactions: towards the control of biochemical pathways.

  2009cites this paper
• Regulation of Morphogenetic Cell Behavior by Xenopus Paraxial

  2009cites this paper
• Nicotiana benthamiana protein, NbPCIP1, interacting with Potato virus X coat protein plays a role as susceptible factor for viral infection.

  2009cites this paper
• Protein complementation assays: Approaches for the in vivo analysis of protein interactions

  2009cites this paper
• Implementing the Bimolecular Fluorescence Complementation assay to study protein interactions in the cell cycle checkpoint response

  2009cites this paper
• Monitoring the interference of protein‐protein interactions in vivo by bimolecular fluorescence complementation: the DnaK case

  2008cites this paper
• Protein complementation assay as a display system for screening protein libraries in the intracellular environment

  2008cites this paper
• The S. Cerevisiae Rho GTPase Cdc42p Is Spatially Regulated by Complex Formation with its GDI Rdi1p and Localization of its GEF Cdc24p

  2008cites this paper
• Protein reporters to study in vivo protein interactions and aggregation

  2008cites this paper