Differential Expression of Ethylene Signaling and Biosynthesis Genes in Floral Organs Between Ethylene-Sensitive and -Insensitive Rose Cultivars

The plant hormone ethylene regulates diverse aspects of plant growth and development, including flower senescence and abscission. In cut roses, the impact of ethylene on flower senescence varies greatly depending on the ethylene sensitivity of the floral organs, which is determined by the variety of the rose. To understand the relationship between ethylene synthesis and sensitivity in floral organs, we examined tissue-specific differences in gene expression and regulation using two rose cultivars with differing ethylene sensitivity. We monitored the expression patterns of genes related to ethylene biosynthesis (RhACS1, RhACS2, RhACS3, RhACS4, and RhACO1), receptor (RhETR1, RhETR2, RhETR3, RhETR4, and RhETR5), and signaling (RhCTR1, RhCTR2, RhEIN3-1, RhEIN3-2, and RhEIN3-3) in various floral organs of ethylene-sensitive (SENS; ‘All For Love’) and -insensitive (INSENS; ‘Peach Valley’) rose cultivars. The expression of ethylene-related genes in all floral organs was generally higher in SENS than INSENS cultivars. The presence of exogenous ethylene accelerated the accumulation of RhACS1-4 and RhACO1 transcripts in SENS petals and leaves and in INSENS stamens and stigmas, leading to an increase in RhETR1-5 expression in the floral organs. Meanwhile, RhCTR1-2 expression decreased considerably, with a consequent increase in RhEIN3 transcripts in SENS petals and leaves and INSENS stamens and stigmas. The differential expression of ethylenerelated genes in the same tissues indicates that the site of initiation of the ethylene-inducible responses varies among the varieties, and this is probably related to the ethylene sensitivity of the flowers. Additional key words: ACC synthase, ethylene receptor, ethylene sensitivity, quantitative RT-PCR, senescence, vase life

• Publication year

  2019

• Venue

  korean Journal of Horticultural Science&Technology

• Publication date

  Unknown publication date

• Fields of study

  Biology, Environmental Science

• Identifiers
  DOI 10.12972/kjhst.20190022
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

• Pre-harvest ethylene control affects vase life of cut rose ‘Carola’ by regulating energy metabolism and antioxidant enzyme activity

  2018cited by this paper
• Potential vase life of cut roses: Seasonal variation and relationships with growth conditions, phenotypes, and gene expressions

  2018cited by this paper
• Relationships between the longevity, water relations, ethylene sensitivity, and gene expression of cut roses

  2017cited by this paper
• Pre-harvest 1-methylcyclopropene application affects post-harvest physiology and storage life of the cut rose cv. Carola

  2017cited by this paper
• Analysis of gene expression during the transition to climacteric phase in carnation flowers (Dianthus caryophyllus L.)

  2013cited by this paper
• Physiology and Biochemistry of Flower Petal Senescence

  2011cited by this paper
• Genotypic Variation in the Postharvest Performance and Ethylene Sensitivity of Cut Rose Flowers

  2010cited by this paper
• Expression of ethylene biosynthetic and receptor genes in rose floral tissues during ethylene-enhanced flower opening

  2008cited by this paper
• Influence of thidiazuron, ethylene, abscisic acid and dark storage on the expression levels of ethylene receptors (ETR) and ACC synthase (ACS) genes in Pelargonium

  2007cited by this paper
• Ethylene-influenced flower opening and expression of genes encoding Etrs, Ctrs, and Ein3s in two cut rose cultivars

  2006cited by this paper
• Transcriptional regulation of ethylene receptor and CTR genes involved in ethylene-induced flower opening in cut rose (Rosa hybrida) cv. Samantha.

  2006cited by this paper
• Cloning of a cDNA encoding an ethylene receptor (DG-ERS1) from chrysanthemum and comparison of its mRNA level in ethylene-sensitive and -insensitive cultivars

  2005cited by this paper
• Differential expression of three genes encoding an ethylene receptor in rice during development, and in response to indole-3-acetic acid and silver ions.

  2004cited by this paper
• Ethylene and flower senescence

  2004cited by this paper
• Biochemical and functional analysis of CTR1, a protein kinase that negatively regulates ethylene signaling in Arabidopsis.

  2003cited by this paper
• Ethylene biosynthetic genes are differentially regulated by ethylene and ACC in carnation styles

  2003cited by this paper
• Ethylene production and enhanced transcription of an ethylene receptor gene, ERS1, in Delphinium during abscission of florets

  2003cited by this paper
• The transcription factor EIN3 is constitutively expressed in miniature roses with differences in postharvest life

  2003cited by this paper
• Localization of the Raf-like Kinase CTR1 to the Endoplasmic Reticulum of Arabidopsis through Participation in Ethylene Receptor Signaling Complexes*

  2003cited by this paper
• Cloning and expression of 1-aminocyclopropane-1-carboxylate synthase cDNA from rosa (Rosa x hybrida)

  2003cited by this paper
• Characterization of two CTR-like protein kinases in Rosa hybrida and their expression during flower senescence and in response to ethylene.

  2002cited by this paper
• Changes in ethylene responsiveness of senescence‐related genes during carnation flower development

  2002cited by this paper
• A two-component gene (NTHK1) encoding a putative ethylene-receptor homolog is both developmentally and stress regulated in tobacco

  2001cited by this paper
• Cultivar differences in regulation of ethylene production in miniature rose flowers ( Rosa hybrida L.)

  2001cited by this paper
• Water Relations of Cut Roses as Influenced by Vapor Pressure Deficits and Temperatures

  2000cited by this paper
• Characterization of an ethylene receptor family with differential expression in rose (Rosa hybrida L.) flowers

  2000cited by this paper
• Stress induced ethylene production, ethylene binding, and the response to the ethylene action inhibitor 1-MCP in miniature roses

  2000cited by this paper
• Role of the gynoecium in natural senescence of carnation (Dianthus caryophyllus L.) flowers.

  2000cited by this paper
• Differential expression of three members of the 1-aminocyclopropane-1-carboxylate synthase gene family in carnation

  1999cited by this paper
• Differential expression of two novel members of the tomato ethylene-receptor family.

  1999cited by this paper
• EIN4 and ERS2 Are Members of the Putative Ethylene Receptor Gene Family in Arabidopsis

  1998cited by this paper
• Differences in display life of miniature potted roses (Rosa hybrida L.)

  1998cited by this paper
• ETR2 is an ETR1-like gene involved in ethylene signaling in Arabidopsis.

  1998cited by this paper
• Ethylene responses are negatively regulated by a receptor gene family in Arabidopsis thaliana.

  1998cited by this paper
• A homolog of the Arabidopsis thaliana ERS gene is actively regulated in Rumex palustris upon flooding.

  1997cited by this paper
• Ethylene biosynthetic genes are differentially expressed during carnation (Dianthus caryophyllus L.) flower senescence

  1997cited by this paper
• Pollination-Induced Ethylene in Carnation (Role of Stylar Ethylene in Corolla Senescence)

  1997cited by this paper
• POLLINATION REGULATION OF FLOWER DEVELOPMENT.

  1997cited by this paper
• Arabidopsis ethylene-response gene ETR1: similarity of product to two-component regulators.

  1993cited by this paper
• Interorgan regulation of ethylene biosynthetic genes by pollination.

  1993cited by this paper
• CTR1, a negative regulator of the ethylene response pathway in Arabidopsis, encodes a member of the raf family of protein kinases.

  1993cited by this paper
• Ethylene Synthesis and Floral Senescence following Compatible and Incompatible Pollinations in Petunia inflata.

  1992cited by this paper
• Synergistic effect of 1-aminocyclopropane-1-carboxylic acid and ethylene during senescence of isolated carnation petals

  1990cited by this paper
• Preserving the Quality of Cold-stored Rose Flowers with Ethylene Antagonists

  1989cited by this paper
• Ethylene and Silver Thiosulfate Influence Opening of Cut Rose Flowers

  1989cited by this paper
• Insensitivity to Ethylene Conferred by a Dominant Mutation in Arabidopsis thaliana

  1988cited by this paper
• Role of Aminocyclopropane-1-carboxylic Acid (ACC) in Control of Ethylene Production in Fresh and Cold-stored Rose Flowers

  1987cited by this paper
• The site of 1‐aminocyclopropane‐1‐carboxylic acid synthesis in senescing carnation petals

  1985cited by this paper
• Partial purification of an ethylene-binding site from Phaseolus vulgaris L. cotyledons

  1985cited by this paper

• Identification of Causal Gene-Specific SNP Markers for the Development of Gynoecious Hybrids in Cucumber (Cucumis sativa L.) Using the PathoLogic Algorithm

  2023cites this paper
• Transcriptome sequencing reveals the senescence mechanism of ethylene-insensitive cut Rhododendron flowers

  2023cites this paper
• Relationship among floral scent intensity, ethylene sensitivity, and longevity of carnation flowers

  2021cites this paper
• Evaluation of Cotton (Gossypium hirsutum L.) Leaf Abscission Sensitivity Triggered by Thidiazuron through Membership Function Value

  2020cites this paper