Site-specific characterization and quantitation of N-glycopeptides in PKM2 knockout breast cancer cells using DiLeu isobaric tags enabled by electron-transfer/higher-energy collision dissociation (EThcD).

No abstract is available for this paper.

• Publication year

  2018

• Venue

  In Analysis

• Publication date

  2018-05-29

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1039/c8an00216aPMID 29687791PMCID PMC5975206
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Recent advances in ion mobility-mass spectrometry for improved structural characterization of glycans and glycoconjugates.

  2018cited by this paper
• PKM2 methylation by CARM1 activates aerobic glycolysis to promote tumorigenesis

  2017cited by this paper
• Improving data quality and preserving HCD-generated reporter ions with EThcD for isobaric tag-based quantitative proteomics and proteome-wide PTM studies.

  2017cited by this paper
• Electron-Transfer/Higher-Energy Collision Dissociation (EThcD)-Enabled Intact Glycopeptide/Glycoproteome Characterization

  2017cited by this paper
• Mass Defect-Based N,N-Dimethyl Leucine Labels for Quantitative Proteomics and Amine Metabolomics of Pancreatic Cancer Cells.

  2017cited by this paper
• Characterization of intact sialylated glycopeptides and phosphorylated glycopeptides from IMAC enriched samples by EThcD fragmentation: Toward combining phosphoproteomics and glycoproteomics

  2017cited by this paper
• Development of a hydrophilic interaction liquid chromatography coupled with matrix-assisted laser desorption/ionization-mass spectrometric imaging platform for N-glycan relative quantitation using stable-isotope labeled hydrazide reagents

  2017cited by this paper
• Sensitive and Precise Characterization of Combinatorial Histone Modifications by Selective Derivatization Coupled with RPLC-EThcD-MS/MS.

  2017cited by this paper
• Maturing Glycoproteomics Technologies Provide Unique Structural Insights into the N-glycoproteome and Its Regulation in Health and Disease*

  2016cited by this paper
• Evaluation of Different N-Glycopeptide Enrichment Methods for N-Glycosylation Sites Mapping in Mouse Brain.

  2016cited by this paper
• Evaluation and Application of Dimethylated Amino Acids as Isobaric Tags for Quantitative Proteomics of the TGF-β/Smad3 Signaling Pathway.

  2016cited by this paper
• Fucosylation of LAMP-1 and LAMP-2 by FUT1 correlates with lysosomal positioning and autophagic flux of breast cancer cells

  2016cited by this paper
• Custom 4-Plex DiLeu Isobaric Labels Enable Relative Quantification of Urinary Proteins in Men with Lower Urinary Tract Symptoms (LUTS)

  2015cited by this paper
• Extended O-GlcNAc on HLA Class-I-Bound Peptides.

  2015cited by this paper
• Development and characterization of novel 8-plex DiLeu isobaric labels for quantitative proteomics and peptidomics.

  2015cited by this paper
• Capillary Electrophoresis-Electrospray Ionization-Mass Spectrometry for Quantitative Analysis of Glycans Labeled with Multiplex Carbonyl-Reactive Tandem Mass Tags.

  2015cited by this paper
• Tissue-Specific Glycosylation at the Glycopeptide Level*

  2015cited by this paper
• Integrated Proteomic and Glycoproteomic Analyses of Prostate Cancer Cells Reveal Glycoprotein Alteration in Protein Abundance and Glycosylation*

  2015cited by this paper
• Glycosylation in cancer: mechanisms and clinical implications

  2015cited by this paper
• Complementary LC-MS/MS-Based N-Glycan, N-Glycopeptide, and Intact N-Glycoprotein Profiling Reveals Unconventional Asn71-Glycosylation of Human Neutrophil Cathepsin G

  2015cited by this paper
• Efficient enrichment of glycopeptides using phenylboronic acid polymer brush modified silica microspheres.

  2014cited by this paper
• Advances in LC-MS/MS-based glycoproteomics: getting closer to system-wide site-specific mapping of the N- and O-glycoproteome.

  2014cited by this paper
• Activation of Akt protects cancer cells from growth inhibition induced by PKM2 knockdown

  2014cited by this paper
• High-Resolution Enabled 12-Plex DiLeu Isobaric Tags for Quantitative Proteomics

  2014cited by this paper
• A Universal Chemical Enrichment Method for Mapping the Yeast N-glycoproteome by Mass Spectrometry (MS)*

  2014cited by this paper
• Abundance-ratio-based semiquantitative analysis of site-specific N-linked glycopeptides present in the plasma of hepatocellular carcinoma patients.

  2014cited by this paper
• Direct approach for qualitative and quantitative characterization of glycoproteins using tandem mass tags and an LTQ Orbitrap XL electron transfer dissociation hybrid mass spectrometer.

  2013cited by this paper
• A simple integrated system for rapid analysis of sialic‐acid‐containing N‐glycopeptides from human serum

  2013cited by this paper
• Brush polymer modified and lectin immobilized core-shell microparticle for highly efficient glycoprotein/glycopeptide enrichment.

  2013cited by this paper
• Unambiguous Phosphosite Localization using Electron-Transfer/Higher-Energy Collision Dissociation (EThcD)

  2013cited by this paper
• N‐linked glycoproteome profiling of human serum using tandem enrichment and multiple fraction concatenation

  2013cited by this paper
• N-linked Glycosylation Enrichment for In-depth Cell Surface Proteomics of Diffuse Large B-cell Lymphoma Subtypes*

  2013cited by this paper
• Structural analysis of glycoprotein sialylation – Part I: pre-LC-MS analytical strategies

  2013cited by this paper
• PKM2, a Central Point of Regulation in Cancer Metabolism

  2013cited by this paper
• Chemical deamidation: a common pitfall in large-scale N-linked glycoproteomic mass spectrometry-based analyses.

  2012cited by this paper
• Mapping N-glycosylation sites across seven evolutionarily distant species reveals a divergent substrate proteome despite a common core machinery.

  2012cited by this paper
• Pyruvate kinase M2 promotes de novo serine synthesis to sustain mTORC1 activity and cell proliferation

  2012cited by this paper
• A two-way street: reciprocal regulation of metabolism and signalling

  2012cited by this paper
• Toward full peptide sequence coverage by dual fragmentation combining electron-transfer and higher-energy collision dissociation tandem mass spectrometry.

  2012cited by this paper
• Regulation of cancer cell metabolism

  2011cited by this paper
• Sialylation and fucosylation of epidermal growth factor receptor suppress its dimerization and activation in lung cancer cells

  2011cited by this paper
• Pyruvate kinase type M2: a key regulator of the metabolic budget system in tumor cells.

  2011cited by this paper
• Evidence for an Alternative Glycolytic Pathway in Rapidly Proliferating Cells

  2010cited by this paper
• Precision mapping of an in vivo N-glycoproteome reveals rigid topological and sequence constraints.

  2010cited by this paper
• Sweetening the pot: adding glycosylation to the biomarker discovery equation.

  2010cited by this paper
• N,N-dimethyl leucines as novel isobaric tandem mass tags for quantitative proteomics and peptidomics.

  2010cited by this paper
• Utilizing ion-pairing hydrophilic interaction chromatography solid phase extraction for efficient glycopeptide enrichment in glycoproteomics.

  2010cited by this paper
• Glycoproteomics in neurodegenerative diseases.

  2010cited by this paper
• Hypoxic regulation of glycosylation via the N-acetylglucosamine cycle

  2010cited by this paper
• Altered Glycosylation in Tumours Focused to Cancer Diagnosis

  2009cited by this paper
• Core fucose and bisecting GlcNAc, the direct modifiers of the N-glycan core: their functions and target proteins.

  2009cited by this paper
• Universal sample preparation method for proteome analysis

  2009cited by this paper
• Cancer cell metabolism: Warburg and beyond.

  2008cited by this paper
• Investigating the biomarker potential of glycoproteins using comparative glycoprofiling - application to tissue inhibitor of metalloproteinases-1.

  2008cited by this paper
• Assessment of lectin and HILIC based enrichment protocols for characterization of serum glycoproteins by mass spectrometry.

  2008cited by this paper
• The M2 splice isoform of pyruvate kinase is important for cancer metabolism and tumour growth

  2008cited by this paper
• Biological function of fucosylation in cancer biology.

  2007cited by this paper
• LAMP proteins are required for fusion of lysosomes with phagosomes

  2007cited by this paper
• Exploring the Sialiome Using Titanium Dioxide Chromatography and Mass Spectrometry *S

  2007cited by this paper
• Remodeling of the lectin–EGF-like domain interface in P- and L-selectin increases adhesiveness and shear resistance under hydrodynamic force

  2006cited by this paper
• Advanced Glycation End Products Enhance Expression of Pro-apoptotic Genes and Stimulate Fibroblast Apoptosis through Cytoplasmic and Mitochondrial Pathways*

  2005cited by this paper
• Protein glycosylation analyzed by normal-phase nano-liquid chromatography--mass spectrometry of glycopeptides.

  2005cited by this paper
• The role of IgG glycoforms in the pathogenesis of rheumatoid arthritis

  2005cited by this paper
• The Coactivator of Transcription CREB-binding Protein Interacts Preferentially with the Glycosylated Form of Stat5*

  2004cited by this paper
• Lectin-based structural glycomics: Glycoproteomics and glycan profiling

  2004cited by this paper
• L- and M2- pyruvate kinase expression in renal cell carcinomas and their metastases

  2004cited by this paper
• A new strategy for identification of N-glycosylated proteins and unambiguous assignment of their glycosylation sites using HILIC enrichment and partial deglycosylation.

  2004cited by this paper
• Screening for N‐glycosylated proteins by liquid chromatography mass spectrometry

  2004cited by this paper
• Regulation of Cytokine Receptors by Golgi N-Glycan Processing and Endocytosis

  2004cited by this paper
• Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry

  2003cited by this paper
• Tumor M2-pyruvate kinase in lung cancer patients: immunohistochemical detection and disease monitoring.

  2002cited by this paper
• Role of LAMP-2 in lysosome biogenesis and autophagy.

  2002cited by this paper
• The multiple roles of PTEN in tumor suppression.

  2000cited by this paper
• Metastases: the glycan connection

  2000cited by this paper
• Accumulation of autophagic vacuoles and cardiomyopathy in LAMP-2-deficient mice

  2000cited by this paper
• Tumor malignancy defined by aberrant glycosylation and sphingo(glyco)lipid metabolism.

  1996cited by this paper
• Assignment of O-glycan attachment sites to the hinge-like regions of human lysosomal membrane glycoproteins lamp-1 and lamp-2.

  1993cited by this paper
• The polylactosaminoglycans of human lysosomal membrane glycoproteins lamp-1 and lamp-2. Localization on the peptide backbones.

  1990cited by this paper
• Appendix 5. Nomenclature for peptide fragment ions (positive ions).

  1990cited by this paper
• A systematic nomenclature for carbohydrate fragmentations in FAB-MS/MS spectra of glycoconjugates

  1988cited by this paper
• [Origin of cancer cells].

  1956cited by this paper
• On the origin of cancer cells.

  1956cited by this paper
• THE METABOLISM OF TUMORS IN THE BODY

  1927cited by this paper

• From identification to quantification: A step-by-step workflow for intact glycopeptide analysis by mass spectrometry.

  2026cites this paper
• The Biological Basis and Analyses of N-Glycan Microheterogeneity

  2025cites this paper
• ExD vs EThcD: What's Better for the Direct Sequencing of Endogenous Amphibian Disulfide Peptides.

  2025cites this paper
• EThcD as a Unique Tool for the Top-Down De Novo Sequencing of Intact Natural Ranid Amphibian Peptides.

  2024cites this paper
• Optimization of glycopeptide enrichment techniques for the identification of clinical biomarkers

  2024cites this paper
• Tools and techniques for quantitative glycoproteomic analysis.

  2024cites this paper
• Differential expression of N‐glycopeptides derived from serum glycoproteins in mild cognitive impairment (MCI) patients

  2024cites this paper
• Analysis of mutation‐originated gain‐of‐glycosylation using mass spectrometry‐based N‐glycoproteomics

  2024influential citation
• Assessing the hydrophobicity of glycopeptides using reversed-phase liquid chromatography and tandem mass spectrometry.

  2023cites this paper
• Structural N-glycoproteomics characterization of cell-surface N-glycosylation of MCF-7/ADR cancer stem cells.

  2023cites this paper
• Measuring change in glycoprotein structure.

  2022cites this paper
• Strategies for Proteome-Wide Quantification of Glycosylation Macro- and Micro-Heterogeneity

  2022cites this paper
• Methods for Quantification of Glycopeptides by Liquid Separation and Mass Spectrometry.

  2022cites this paper
• Recent development in hydrophilic interaction liquid chromatography stationary materials for glycopeptide analysis.

  2022cites this paper
• Boost-DiLeu: Enhanced Isobaric N,N-Dimethyl Leucine Tagging Strategy for a Comprehensive Quantitative Glycoproteomic Analysis.

  2022cites this paper
• Advances in mass spectrometry‐based glycoproteomics: An update covering the period 2017–2021

  2021cites this paper
• In-depth Site-specific Analysis of N-glycoproteome in Human Cerebrospinal Fluid and Glycosylation Landscape Changes in Alzheimer's Disease

  2021cites this paper
• Benchmark of site- and structure-specific quantitative tissue N-glycoproteomics for discovery of potential N-glycoprotein markers: a case study of pancreatic cancer

  2021cites this paper
• Posttranslational modification of pyruvate kinase type M2 (PKM2): novel regulation of its biological roles to be further discovered

  2021cites this paper
• Methods to improve quantitative glycoprotein coverage from bottom-up LC-MS data.

  2021cites this paper
• Recent Advances in Software Tools for More Generic and Precise Intact Glycopeptide Analysis

  2021cites this paper
• Towards structure-focused glycoproteomics

  2021cites this paper
• Comparative analysis of intact glycopeptides from mannose receptor among different breast cancer subtypes using mass spectrometry.

  2021cites this paper
• In-Depth Site-Specific O-Glycosylation Analysis of Glycoproteins and Endogenous Peptides in Cerebrospinal Fluid (CSF) from Healthy Individuals, Mild Cognitive Impairment (MCI), and Alzheimer's Disease (AD) Patients.

  2021cites this paper
• One step carboxyl group isotopic labeling for quantitative analysis of intact N-glycopeptides by mass spectrometry.

  2021cites this paper
• Systematic Evaluation of Fragmentation Methods for Unlabeled and Isobaric Mass Tag-Labeled O-Glycopeptides.

  2021cites this paper
• MS-based glycomics and glycoproteomics methods enabling isomeric characterization.

  2021cites this paper
• Recent Advances in Mass Spectrometry-Based Glycomic and Glycoproteomic Studies of Pancreatic Diseases

  2021cites this paper
• Evaluating the Performance of 193 nm Ultraviolet Photodissociation for Tandem Mass Tag Labeled Peptides

  2021cites this paper
• Data-Independent Acquisition-Based Mass Spectrometry (DIA-MS) for Quantitative Analysis of Intact N-Linked Glycopeptides.

  2021cites this paper
• A Pragmatic Guide to Enrichment Strategies for Mass Spectrometry-based Glycoproteomics.

  2020cites this paper
• Recent advances in hydrophilic interaction liquid interaction chromatography materials for glycopeptide enrichment and glycan separation

  2020cites this paper
• Quantitative site- and structure-specific N-glycoproteomics characterization of differential N-glycosylation in MCF-7/ADR cancer stem cells

  2020cites this paper
• A quantitative N-glycoproteomics study of cell-surface N-glycoprotein markers of MCF-7/ADR cancer stem cells

  2020cites this paper
• The effectiveness of filtering glycopeptide peak list files for Y ions.

  2020cites this paper
• Use of lectin-based affinity techniques in breast cancer glycoproteomics - Minireview.

  2020cites this paper
• Isobaric labeling strategy utilizing 4-plex N,N-dimethyl leucine (DiLeu) tags reveals proteomic changes induced by chemotherapy in cerebrospinal fluid of children with B-cell acute lymphoblastic leukemia.

  2020cites this paper
• Glycopeptide Biomarkers in Serum Haptoglobin for Hepatocellular Carcinoma Detection in Patients with Non-Alcoholic Steatohepatitis.

  2020cites this paper
• Optimal Dissociation Methods Differ for N- and O-Glycopeptides

  2020cites this paper
• Recent Advances in Analytical Approaches for Glycan and Glycopeptide Quantitation

  2020cites this paper
• Recent advances in software tools for more generic and precise intact glycopeptide analysis.

  2020cites this paper
• Comprehensive structural glycomic characterization of the glycocalyxes of cells and tissues

  2020cites this paper
• A Pragmatic Guide to Enrichment Strategies for Mass Spectrometry–Based Glycoproteomics

  2020cites this paper
• Signature-Ion Triggered Mass Spectrometry Approach Enabled Discovery of N- and O-linked Glycosylated Neuropeptides in the Crustacean Nervous System.

  2019cites this paper
• Site- and structure-specific quantitative N-glycoproteomics study of differential N-glycosylation in MCF-7 cancer cells.

  2019cites this paper
• Hydrophilic interaction chromatography for the analysis of biopharmaceutical drugs and therapeutic peptides: A review based on the separation characteristics of the hydrophilic interaction chromatography phases.

  2019cites this paper
• Site- and structure-specific characterization of N-glycoprotein markers of MCF-7 cancer stem cells using isotopic-labelling quantitative N-glycoproteomics.

  2019cites this paper
• Quantitative Proteomics for Analyses of Multiple Samples in Parallel with Chemical Perturbation

  2019cites this paper
• Mass spectrometry imaging of N-glycans from formalin-fixed paraffin-embedded tissue sections using a novel sub-atmospheric pressure ionization source.

  2019cites this paper
• Site‐ and Structure‐Specific Quantitative N‐Glycoproteomics Using RPLC‐pentaHILIC Separation and the Intact N‐Glycopeptide Search Engine GPSeeker

  2019cites this paper
• Systematic and site-specific analysis of N-glycoproteins on the cell surface by integrating bioorthogonal chemistry and MS-based proteomics.

  2019cites this paper
• Characterization of Cell Glycocalyx with Mass Spectrometry Methods

  2019cites this paper
• Human protein paucimannosylation: cues from the eukaryotic kingdoms

  2019cites this paper
• Dual-Functional Titanium (IV) Immobilized Metal Affinity Chromatography Enables Large-scale Profiling of Protein Mannose-6-Phosphate Glycosylation and Reveals Its Predominant Substrates.

  2019cites this paper
• Towards a standardized bioinformatics infrastructure for N- and O-glycomics

  2019cites this paper
• Simply Extending the Mass Range in Electron Transfer Higher Energy Collisional Dissociation Increases Confidence in N-Glycopeptide Identification

  2019cites this paper
• Fluorescent visual quantitation of cell-secreted sialoglycoconjugates by chemoselective recognition and hybridization chain reaction.

  2019cites this paper
• GPSeeker Enables Quantitative Structural N-glycoproteomics for site- and structure-specific characterization of differentially expressed N-glycosylation in hepatocellular carcinoma.

  2019cites this paper
• Recent advances in mass spectrometry (MS)-based glycoproteomics in complex biological samples.

  2019cites this paper
• Glycoproteomic markers of hepatocellular carcinoma-mass spectrometry based approaches.

  2018cites this paper
• The benefits of hybrid fragmentation methods for glycoproteomics

  2018cites this paper
• Sample Multiplexing Strategies in Quantitative Proteomics.

  2018cites this paper
• Differential Quantitative Determination of Site-Specific Intact N-Glycopeptides in Serum Haptoglobin between Hepatocellular Carcinoma and Cirrhosis Using LC-EThcD-MS/MS.

  2018cites this paper