Effects of polysaccharides from Inonotus obliquus and its chromium (III) complex on advanced glycation end-products formation, α-amylase, α-glucosidase activity and H2O2-induced oxidative damage in hepatic L02 cells.

In the present study, the antioxidant activity, anti-glycation activity, α-amylase, α-glucosidase inhibitory activity of polysaccharides from Inonotus obliquus (UIOPS) and its chromium (III) complex (UIOPC) were investigated. Their protective effects against H2O2-induced oxidative damages in hepatic L02 cells were also assessed. Results demonstrated that UIOPC and UIOPS exhibited remarkable DPPH scavenging activity, ferric reducing power and hemolysis inhibitory activity. UIOPC also showed significant inhibitory capacity on α-amylase and α-glucosidase than UIOPS (P < 0.05), suggesting a good regulation of the postprandial hyperglycemia. Three phases of advanced glycation end products (AGEs) formation were effectively inhibited by UIOPC and UIOPS. Moreover, pretreatment with UIOPC and UIOPS markedly attenuated the oxidative damage induced by H2O2 in hepatic L02 cells via enhancing the cell viability, inhibiting the morphology alteration and maintaining the integrity of mitochondria. These results indicated that the anti-diabetic mechanism of UIOPC might involve in the homoeostasis of blood glucose and the recovery of endogenous antioxidant system. The elucidation of the potential anti-diabetic mechanism will facilitate the further study and application of the polysaccharides-metal complex in the functional food industry.

• Publication year

  2018

• Venue

  Food and Chemical Toxicology

• Publication date

  2018-06-01

• Fields of study

  Medicine, Chemistry, Environmental Science

• Identifiers
  DOI 10.1016/j.fct.2018.04.047PMID 29689356
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• In vitro inhibition of pancreatic α-amylase by spherical and polygonal starch nanoparticles.

  2018cited by this paper
• Anti-diabetic effects of Inonotus obliquus polysaccharides in streptozotocin-induced type 2 diabetic mice and potential mechanism via PI3K-Akt signal pathway.

  2017cited by this paper
• Fractionation, preliminary structural characterization and bioactivities of polysaccharides from Sargassum pallidum.

  2017cited by this paper
• Oxidative toxicity in diabetes and Alzheimer’s disease: mechanisms behind ROS/ RNS generation

  2017cited by this paper
• Differential α-amylase/α-glucosidase inhibitory activities of plant-derived phenolic compounds: a virtual screening perspective for the treatment of obesity and diabetes.

  2017cited by this paper
• Anti-diabetic effects of Inonotus obliquus polysaccharides-chromium (III) complex in type 2 diabetic mice and its sub-acute toxicity evaluation in normal mice.

  2017cited by this paper
• Sea Buckthorn Fruit Oil Extract Alleviates Insulin Resistance through the PI3K/Akt Signaling Pathway in Type 2 Diabetes Mellitus Cells and Rats.

  2017cited by this paper
• Ball milling improves extractability and antioxidant properties of the active constituents of mushroom Inonotus obliquus powders

  2016cited by this paper
• Intracellular ROS scavenging and antioxidant enzyme regulating capacities of corn gluten meal-derived antioxidant peptides in HepG2 cells.

  2016cited by this paper
• Grape skin phenolics as inhibitors of mammalian α-glucosidase and α-amylase--effect of food matrix and processing on efficacy.

  2016cited by this paper
• Inhibition of Advanced Glycation End-Product Formation and Antioxidant Activity by Extracts and Polyphenols from Scutellaria alpina L. and S. altissima L.

  2016cited by this paper
• In vitro inhibitory activities of selected Australian medicinal plant extracts against protein glycation, angiotensin converting enzyme (ACE) and digestive enzymes linked to type II diabetes

  2016cited by this paper
• Advanced Glycation End Products and Oxidative Stress in Type 2 Diabetes Mellitus

  2015cited by this paper
• Controlling diabetes by chromium complexes: The role of the ligands.

  2015cited by this paper
• Diabetes mellitus and oxidative stress—A concise review

  2015cited by this paper
• Polysaccharide from Inonotus obliquus inhibits migration and invasion in B16-F10 cells by suppressing MMP-2 and MMP-9 via downregulation of NF-κB signaling pathway.

  2014cited by this paper
• In vitro antioxidant, anti-diabetic, cholinesterase and tyrosinase inhibitory potential of fresh juice from Citrus hystrix and C. maxima fruits

  2014cited by this paper
• Physicochemical characterization of puerh tea polysaccharides and their antioxidant and α-glycosidase inhibition

  2014cited by this paper
• Characterization and antioxidant activities of degraded polysaccharides from two marine Chrysophyta.

  2014cited by this paper
• N-Acetyl-Serotonin Protects HepG2 Cells from Oxidative Stress Injury Induced by Hydrogen Peroxide

  2014cited by this paper
• Investigating Wild Berries as a Dietary Approach to Reducing the Formation of Advanced Glycation Endproducts: Chemical Correlates of In Vitro Antiglycation Activity

  2014cited by this paper
• Protective Effect of Polysaccharides from Inonotus obliquus on Streptozotocin-Induced Diabetic Symptoms and Their Potential Mechanisms in Rats

  2014cited by this paper
• Neuroprotective effects of corn silk maysin via inhibition of H2O2-induced apoptotic cell death in SK-N-MC cells.

  2014cited by this paper
• Blockade of SOCE protects HT22 cells from hydrogen peroxide-induced apoptosis.

  2013cited by this paper
• Physical modifications of polysaccharide from Inonotus obliquus and the antioxidant properties.

  2013cited by this paper
• Tuning a 96-Well Microtiter Plate Fluorescence-Based Assay to Identify AGE Inhibitors in Crude Plant Extracts

  2013cited by this paper
• Advanced glycation endproducts in diabetes and diabetic complications.

  2013cited by this paper
• Role of chromium in human health and in diabetes. Diabetes Care 2004;27:2741–2751

  2013cited by this paper
• Protective effects of sulfated chitooligosaccharides with different degrees of substitution in MIN6 cells.

  2013cited by this paper
• Chemical modification and antioxidant activities of polysaccharide from mushroom Inonotus obliquus.

  2012cited by this paper
• Inhibitory effect of polysaccharides from pumpkin on advanced glycation end-products formation and aldose reductase activity

  2012cited by this paper
• Antitumor and immunomodulatory activity of water-soluble polysaccharide from Inonotus obliquus.

  2012cited by this paper
• Antioxidation and antiglycation of polysaccharides from Misgurnus anguillicaudatus

  2011cited by this paper
• Phytochemical characteristics and hypoglycaemic activity of fraction from mushroom Inonotus obliquus.

  2010cited by this paper
• Antihyperglycemic, Antihyperlipidemic and Antiglycation Effects of Byrsonima crassifolia Fruit and Seed in Normal and Streptozotocin-Induced Diabetic Rats

  2010cited by this paper
• Methazolamide and melatonin inhibit mitochondrial cytochrome C release and are neuroprotective in experimental models of ischemic injury.

  2009cited by this paper
• DPPH antioxidant assay revisited

  2009cited by this paper
• Upregulation of oxidative stress markers in human microvascular endothelial cells by complexes of serum albumin and digestion products of glycated casein

  2009cited by this paper
• Inhibitory effects of guava (Psidium guajava L.) leaf extracts and its active compounds on the glycation process of protein

  2009cited by this paper
• Protective effects of salidroside on hydrogen peroxide-induced apoptosis in SH-SY5Y human neuroblastoma cells.

  2007cited by this paper
• Antioxidant small phenolic ingredients in Inonotus obliquus (persoon) Pilat (Chaga).

  2007cited by this paper
• Chemical studies on antioxidant mechanisms and free radical scavenging properties of lignans.

  2005cited by this paper
• Inhibitory effects of Luobuma tea and its components against glucose-mediated protein damage.

  2004cited by this paper
• Role of Chromium in Human Health and in Diabetes

  2004cited by this paper
• Quercetin protects the hydrogen peroxide-induced apoptosis via inhibition of mitochondrial dysfunction in H9c2 cardiomyoblast cells.

  2003cited by this paper
• Human Glycated Albumin Affects Glucose Metabolism in L6 Skeletal Muscle Cells by Impairing Insulin-induced Insulin Receptor Substrate (IRS) Signaling through a Protein Kinase Cα-mediated Mechanism*

  2003cited by this paper
• An In Vitro Model of Parkinson's Disease: Linking Mitochondrial Impairment to Altered α-Synuclein Metabolism and Oxidative Damage

  2002cited by this paper
• The structure–activity relationships of dicarbonyl compounds and their role in the Maillard reaction

  2002cited by this paper
• Advanced glycation end-products: a review

  2001cited by this paper
• Amadori-glycated albumin in diabetic nephropathy: pathophysiologic connections.

  2000cited by this paper
• Chromium in the prevention and control of diabetes.

  2000cited by this paper
• Ferric reducing/antioxidant power assay: direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration.

  1999cited by this paper
• BCL-2 expression or antioxidants prevent hyperglycemia-induced formation of intracellular advanced glycation endproducts in bovine endothelial cells.

  1996cited by this paper
• Mitochondria and programmed cell death: back to the future

  1996cited by this paper
• Mechanism of autoxidative glycosylation: identification of glyoxal and arabinose as intermediates in the autoxidative modification of proteins by glucose.

  1995cited by this paper
• Chemistry of the fructosamine assay: D-glucosone is the product of oxidation of Amadori compounds.

  1994cited by this paper
• Glucose autoxidation and protein modification. The potential role of 'autoxidative glycosylation' in diabetes.

  1987cited by this paper

• Advancements in pharmaceutical research on traditional Chinese medicine-metal complexes

  2026cites this paper
• Advances in Chemically Modified Polysaccharides with Enhanced Hypoglycemic Bioactivity.

  2026cites this paper
• Preparing a millet bran soluble dietary fiber polysaccharide-Cr (III) complex to regulate oxidative stress and apoptosis in pancreatic islet cells.

  2025cites this paper
• Construction of modified chitosan-ovalbumin mixed systems: enhancing quercetin delivery potential, stability and anti-glycation effects.

  2025cites this paper
• Association between blood chromium and hepatic steatosis assessed by liver ultrasound transient elastography: National Health and Nutrition Examination Survey 2017–2020

  2024cites this paper
• Protective effect of Inonotus obliquus polysaccharide on MGO-induced nonenzymatic glycation fibroblasts

  2024cites this paper
• Potential inhibitory effect of Auricularia auricula polysaccharide on advanced glycation end-products (AGEs).

  2024cites this paper
• Research on Chaga hypoglycemic functional foods

  2024cites this paper
• Advances in dietary polysaccharides as hypoglycemic agents: mechanisms, structural characteristics, and innovative applications

  2023influential citation
• Developing ginger polysaccharide-Cr (III) complexes using ginger peel to provide enhanced in vivo anti-inflammatory activity: Fabrication, structural characterization and activity evaluation

  2023cites this paper
• Influence of Five Drying Methods on Active Compound Contents and Bioactivities of Fresh Flowers from Syringa pubescens Turcz

  2023cites this paper
• Blueberry anthocyanins extract inhibits advanced glycation end-products (AGEs) production and AGEs-stimulated inflammation in RAW264.7 cells.

  2023cites this paper
• Polysaccharides from natural resource: ameliorate type 2 diabetes mellitus via regulation of oxidative stress network

  2023cites this paper
• Garlic polysaccharide-Cr (III) complexes with enhanced in vitro and in vivo hypoglycemic activities.

  2023cites this paper
• Compositional analysis and immunomodulatory activity of blue pigment fraction (BPF) from Laba garlic.

  2022cites this paper
• Photoprotective Potential, Cytotoxicity, and UPLC-QTOF/MS Analysis on Bioactive Solvent Fractions of Moringa concanensis Nimmo Bark

  2022cites this paper
• Effect of Inonotus obliquus Extract Supplementation on Endurance Exercise and Energy-Consuming Processes through Lipid Transport in Mice

  2022cites this paper
• The antioxidant activities, inhibitory effects, kinetics, and mechanisms of artocarpin and α-mangostin on α-glucosidase and α-amylase.

  2022cites this paper
• Synthesis, Characterization, and Bioactivities of Polysaccharide Metal Complexes: A Review.

  2022cites this paper
• Glycation-induced age-related illnesses, antiglycation and drug delivery strategies.

  2022cites this paper
• Studies on the key constituents and the related mechanisms of Clerodendranthus spicatus in the treatment of diabetes based on network pharmacology.

  2022cites this paper
• Effect of Fe (III), Zn (II), and Cr (III) complexation on the physicochemical properties and bioactivities of corn silk polysaccharide.

  2021cites this paper
• Garlic flavonoids alleviate H2 O2 induced oxidative damage in L02 cells and induced apoptosis in HepG2 cells by Bcl-2/Caspase pathway.

  2021cites this paper
• A polysaccharide of PFP-1 from Pleurotus geesteranus attenuates alcoholic liver diseases via Nrf2 and NF-κB signaling pathways.

  2021cites this paper
• Recent Developments in Inonotus obliquus (Chaga mushroom) Polysaccharides: Isolation, Structural Characteristics, Biological Activities and Application

  2021cites this paper
• An Overview of Polysaccharides and the Influence Factors of Hypoglycemic Activity

  2021cites this paper
• Effects of Polysaccharides on Reducing Blood Glucose Based on Gut Microbiota Alteration

  2021cites this paper
• Chrysophyllum cainito. L alleviates diabetic and complications by playing antioxidant, antiglycation, hypoglycemic roles and the chemical profile analysis.

  2021cites this paper
• Glycation and Antioxidants: Hand in glove of antiglycation and natural antioxidants.

  2020cites this paper
• Inonotus obliquus – from folk medicine to clinical use

  2020cites this paper
• Therapeutic potential of mushrooms in diabetes mellitus: Role of polysaccharides.

  2020cites this paper
• Highly modified steroids from Inonotus obliquus.

  2020cites this paper
• Synthesis, characterization and biological assay of three new benzotriazole-based Zn(II) complexes

  2020cites this paper
• Study on the active polyphenol constituents in differently colored Rubus Chingii Hu and the structure-activity relationship of the main ellagitannins and ellagic acid

  2020cites this paper
• Antioxidant, α-amylase and α-glucosidase activity of various solvent fractions of I. obliquus and the preventive role of active fraction against H2 O2 induced damage in hepatic L02 cells as fungisome.

  2020cites this paper
• Preparation of a Momordica charantia L. polysaccharide‑chromium (III) complex and its anti-hyperglycemic activity in mice with streptozotocin-induced diabetes.

  2019cites this paper
• Antioxidant and antihypertensive effects of garlic protein and its hydrolysates and the related mechanism.

  2019cites this paper
• Protective effects of polysaccharides on hepatic injury: A review.

  2019cites this paper
• Effects of N-trans-feruloyltyramine isolated from laba garlic on antioxidant, cytotoxic activities and H2O2-induced oxidative damage in HepG2 and L02 cells.

  2019cites this paper
• Structural characterization of corn silk polysaccharides and its effect in H2O2 induced oxidative damage in L6 skeletal muscle cells.

  2019cites this paper
• Inhibitory effects of polysaccharide from Diaphragma juglandis fructus on α-amylase and α-d-glucosidase activity, streptozotocin-induced hyperglycemia model, advanced glycation end-products formation, and H2O2-induced oxidative damage.

  2019cites this paper
• Hypoglycemic effects of polysaccharides from corn silk (Maydis stigma) and their beneficial roles via regulating the PI3K/Akt signaling pathway in L6 skeletal muscle myotubes.

  2019cites this paper
• Structure Characterization and Hypoglycaemic Activities of Two Polysaccharides from Inonotus obliquus

  2018cites this paper
• Protective effect of polysaccharide from Agaricus bisporus in Tibet area of China against tetrachloride-induced acute liver injury in mice.

  2018cites this paper