Multiplexable fluorescence lifetime imaging (FLIM) probes for Syk and Src-family kinases

Most commonly employed strategies to map kinase activities in live cells require expression of genetically-encoded proteins (e.g. FRET sensors). In this work, we describe development and preliminary application of a set of cell-penetrating, fluorophore labelled peptide substrates for fluorescence lifetime imaging (FLIM) of Syk and Src-family kinase activities. These probes do not rely on FRET pairs or genetically-encoded protein expression. We also demonstrate image-by-image and pixel-by-pixel quantification of probe phosphorylation ratio, suggesting that this strategy will be useful for detailed mapping of single cell and subcellular kinase activation in live cells.

• Publication year

  2019

• Venue

  bioRxiv

• Publication date

  2019-05-30

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1101/655407PMID 33035286PMCID PMC7679178
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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