Two new colonial peritrich ciliates (Ciliophora, Peritrichia, Sessilida) from China: With a note on taxonomic distinction between Epistylididae and Operculariidae.

Two new colonial sessilid species, Opercularia miaoxinensis spec. nov. and Epistylis conica spec. nov., were isolated from the pereopods of freshwater crayfish, Procambarus clarkii in Hubei Province, China from 2016 to 2017. Both species were investigated by living observation, protargol impregnation, and molecular methods. Opercularia miaoxinensis spec. nov. is morphologically characterized by the following characteristics: spindle-shaped zooid, zooid size of 48-74 × 20-35 μm in vivo, contractile vacuole ventrally located above macronucleus, and dichotomously branched stalk. Epistylis conica spec. nov. is characterized by conical zooid shape, zooid size of 56-70 × 22-39 μm in vivo, C-shaped macronucleus with transverse orientation, dorsally located contractile vacuole, and dichotomously branched stalk. To further identify both species, phylogenetic trees were constructed based on small subunit ribosomal DNA (SSU rDNA) and ITS1-5.8S-ITS2 sequences. Both results showed that E. conica spec. nov. was part of a clade consisting of the majority of Epistylis species. Surprisingly, O. miaoxinensis spec. nov. also clustered within this large clade of Epistylis species and had a distant relationship with the other Opercularia species. These findings challenged the distinguishing morphological characteristics between Epistylididae and Operculariidae.

• Publication year

  2019

• Venue

  European Journal of Protistology

• Publication date

  2019-08-01

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1016/J.EJOP.2019.05.003PMID 31352233
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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