Microfocus x-ray fluorescence mapping of tumour penetration by an organo‑osmium anticancer complex.

Microfocus synchrotron x-ray fluorescence (SXRF) imaging focussed on detection of the Os LIII edge shows that the organo‑osmium metallodrug candidate [(ŋ6-p-cym)Os(Azpy-NMe2)I]+ (p-cym = p-cymene, Azpy-NMe2 = 2-(p-([dimethylamino]phenylazo)pyridine)) [1] penetrates efficiently into the interior of A2780 human ovarian cancer cell spheroids, a model for a solid tumour. The accompanying changes in Zn and Ca distribution suggest that the complex causes nuclear damage and initiates signalling events for cell death, consistent with findings for cultured cancer cell monolayers. Such tumour penetration is likely to be important for combatting resistance to chemotherapy, which is becoming a problem for current clinical platinum drugs.

• Publication year

  2018

• Venue

  Journal of Inorganic Biochemistry

• Publication date

  2018-08-01

• Fields of study

  Medicine, Chemistry

• Identifiers
  DOI 10.1016/j.jinorgbio.2018.04.014PMID 29730234
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Synchrotron X‐Ray Fluorescence Nanoprobe Reveals Target Sites for Organo‐Osmium Complex in Human Ovarian Cancer Cells

  2017cited by this paper
• LA-ICP-MS imaging in multicellular tumor spheroids - a novel tool in the preclinical development of metal-based anticancer drugs.

  2016cited by this paper
• University of Birmingham The contrasting catalytic efficiency and cancer cell antiproliferative activity of stereoselective organoruthenium transfer hydrogenation catalysts

  2016cited by this paper
• In‐Cell Activation of Organo‐Osmium(II) Anticancer Complexes

  2016cited by this paper
• Preserving elemental content in adherent mammalian cells for analysis by synchrotron‐based x‐ray fluorescence microscopy

  2016cited by this paper
• Chemical Imaging of Platinum-Based Drugs and their Metabolites

  2016cited by this paper
• Increasing anti-cancer activity with longer tether lengths of group 9 Cp* complexes.

  2016cited by this paper
• Potent organo-osmium compound shifts metabolism in epithelial ovarian cancer cells

  2015cited by this paper
• Enhancement of Selectivity of an Organometallic Anticancer Agent by Redox Modulation

  2015cited by this paper
• Organoiridium Complexes: Anticancer Agents and Catalysts

  2014cited by this paper
• An organometallic inhibitor for the human repair enzyme 7,8-dihydro-8-oxoguanosine triphosphatase.

  2014cited by this paper
• Potent organometallic osmium compounds induce mitochondria-mediated apoptosis and S-phase cell cycle arrest in A549 non-small cell lung cancer cells.

  2014cited by this paper
• Organometallic anticancer complexes of lapachol: metal centre-dependent formation of reactive oxygen species and correlation with cytotoxicity.

  2013cited by this paper
• A New Age for Iron: Antitumoral Ferrocenes

  2013cited by this paper
• Opportunities and challenges for use of tumor spheroids as models to test drug delivery and efficacy.

  2012cited by this paper
• Getting to the core of platinum drug bio-distributions: the penetration of anti-cancer platinum complexes into spheroid tumour models.

  2012cited by this paper
• Anti-colorectal cancer activity of an organometallic osmium arene azopyridine complex

  2011cited by this paper
• Multicellular tumor spheroids: an underestimated tool is catching up again.

  2010cited by this paper
• Organometallic osmium arene complexes with potent cancer cell cytotoxicity.

  2010cited by this paper
• Organometallic Anticancer Compounds

  2010cited by this paper
• Activation Mechanisms for Organometallic Anticancer Complexes

  2010cited by this paper
• Anticancer activity of new organo-ruthenium, rhodium and iridium complexes containing the 2-(pyridine-2-yl)thiazole N,N -chelating ligand

  2010cited by this paper
• I18--the microfocus spectroscopy beamline at the Diamond Light Source.

  2009cited by this paper
• Bioorganometallic chemistry--from teaching paradigms to medicinal applications.

  2009cited by this paper
• The ruthenium(II)–arene compound RAPTA-C induces apoptosis in EAC cells through mitochondrial and p53–JNK pathways

  2008cited by this paper
• Elemental tomography of cancer-cell spheroids reveals incomplete uptake of both platinum(II) and platinum(IV) complexes.

  2007cited by this paper
• The resurgence of platinum-based cancer chemotherapy

  2007cited by this paper
• Drug penetration in solid tumours

  2006cited by this paper

• Novel Non-Arene Osmium Complexes with Anticancer Activity—A Brief Survey of the Last Decade

  2026cites this paper
• Cancer 3D Models for Metallodrug Preclinical Testing

  2023cites this paper
• Cellular detection of a mitochondria targeted brominated vinyl triphenylamine optical probe (TP-Br) by X-ray fluorescence microscopy.

  2022cites this paper
• Exact Two-Component TDDFT with Simple Two-Electron Picture-Change Corrections: X-ray Absorption Spectra Near L- and M-Edges of Four-Component Quality at Two-Component Cost

  2022cites this paper
• Approaches towards understanding the mechanism-of-action of metallodrugs

  2021cites this paper
• Os(II)-Bridged Polyarginine Conjugates: The Additive Effects of Peptides in Promoting or Preventing Permeation in Cells and Multicellular Tumor Spheroids

  2021cites this paper
• Application of X-ray absorption and X-ray fluorescence techniques to the study of metallodrug action.

  2021cites this paper
• X-ray-Based Techniques to Study the Nano–Bio Interface

  2021cites this paper
• Elemental mapping of half-sandwich azopyridine osmium arene complexes in cancer cells

  2021cites this paper
• Oxadiazole based Os(IV) compounds as potential DNA intercalator and cytotoxic agents

  2020cites this paper
• Tracking Metal Ions in Biology UsingX‐Ray Methods

  2019cites this paper
• Nanofocused synchrotron X-ray absorption studies of the intracellular redox state of an organometallic complex in cancer cells.

  2019cites this paper
• Recent Trends in Potential Therapeutic Applications of the Dietary Flavonoid Didymin

  2018cites this paper