X‐ray and Cryo‐EM structures reveal mutual conformational changes of Kinesin and GTP‐state microtubules upon binding

No abstract is available for this paper.

• Publication year

  2015

• Venue

  EMBO Journal

• Publication date

  2015-03-17

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.15252/embj.201490588PMID 25777528PMCID PMC4426485
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• High-resolution structures of kinesin on microtubules provide a basis for nucleotide-gated force-generation

  2014cited by this paper
• New simulated annealing approach considering helix bending applied to determine the 8.8Å structure of 15-protofilament microtubules.

  2014cited by this paper
• High-resolution microtubule structures reveal the structural transitions in αβ-tubulin upon GTP hydrolysis.

  2014cited by this paper
• Conserved mechanisms of microtubule-stimulated ADP release, ATP binding, and force generation in transport kinesins

  2014influential reference
• The structure of apo-kinesin bound to tubulin links the nucleotide cycle to movement

  2014cited by this paper
• Structure of a kinesin–tubulin complex and implications for kinesin motility

  2013cited by this paper
• Structural basis for the ATP-induced isomerization of kinesin.

  2013cited by this paper
• Conformational changes in tubulin in GMPCPP and GDP-taxol microtubules observed by cryoelectron microscopy

  2012influential reference
• Preferential binding of a kinesin-1 motor to GTP-tubulin–rich microtubules underlies polarized vesicle transport

  2011influential reference
• REFMAC5 for the refinement of macromolecular crystal structures

  2011cited by this paper
• Using Sculptor and Situs for simultaneous assembly of atomic components into low-resolution shapes.

  2011influential reference
• Features and development of Coot

  2010cited by this paper
• An atomic-level mechanism for activation of the kinesin molecular motors

  2010cited by this paper
• Structural and functional insights into the Myosin motor mechanism.

  2010cited by this paper
• Kinesin superfamily motor proteins and intracellular transport

  2009cited by this paper
• The mechanisms of kinesin motor motility: lessons from the monomeric motor KIF1A

  2009cited by this paper
• Structural model for strain-dependent microtubule activation of Mg-ADP release from kinesin

  2008influential reference
• An ATP Gate Controls Tubulin Binding by the Tethered Head of Kinesin-1

  2007cited by this paper
• The beginning of kinesin's force-generating cycle visualized at 9-Å resolution

  2007cited by this paper
• Large conformational changes in a kinesin motor catalyzed by interaction with microtubules.

  2006cited by this paper
• A fully automatic 3D reconstruction method using simulated annealing enables accurate posterioric angular assignment of protein projections.

  2006cited by this paper
• High‐resolution cryo‐EM maps show the nucleotide binding pocket of KIF1A in open and closed conformations

  2006cited by this paper
• A change in the selective translocation of the Kinesin-1 motor domain marks the initial specification of the axon.

  2006cited by this paper
• Kinesin Walks Hand-Over-Hand

  2004cited by this paper
• Structure of a kinesin microtubule depolymerization machine

  2004cited by this paper
• KIF1A Alternately Uses Two Loops to Bind Microtubules

  2004cited by this paper
• UCSF Chimera—A visualization system for exploratory research and analysis

  2004cited by this paper
• Microtubules provide directional cues for polarized axonal transport through interaction with kinesin motor head

  2003cited by this paper
• Kinesin Moves by an Asymmetric Hand-Over-Hand Mechanism

  2003cited by this paper
• Purification of brain tubulin through two cycles of polymerization-depolymerization in a high-molarity buffer.

  2003cited by this paper
• A structural state of the myosin V motor without bound nucleotide

  2003cited by this paper
• Crystallographic findings on the internally uncoupled and near-rigor states of myosin: Further insights into the mechanics of the motor

  2002cited by this paper
• Refined structure of alpha beta-tubulin at 3.5 A resolution.

  2001cited by this paper
• An improved microscope for bead and surface-based motility assays.

  2001cited by this paper
• Switch-based mechanism of kinesin motors

  2001cited by this paper
• A structural change in the kinesin motor protein that drives motility

  1999cited by this paper
• A processive single-headed motor: kinesin superfamily protein KIF1A.

  1999cited by this paper
• Direction determination in the minus-end-directed kinesin motor ncd

  1998cited by this paper
• Preparation and characterization of caged fluorescein tubulin.

  1998cited by this paper
• Processing of X-ray diffraction data collected in oscillation mode.

  1997cited by this paper
• Crystal structure of the kinesin motor domain reveals a structural similarity to myosin

  1996cited by this paper
• NMRPipe: A multidimensional spectral processing system based on UNIX pipes

  1995cited by this paper
• Tubulin GTP hydrolysis influences the structure, mechanical properties, and kinesin-driven transport of microtubules.

  1994cited by this paper
• Yeast Kar3 is a minus‐end microtubule motor protein that destabilizes microtubules preferentially at the minus ends.

  1994cited by this paper
• Comparative protein modelling by satisfaction of spatial restraints.

  1993cited by this paper
• Bead movement by single kinesin molecules studied with optical tweezers

  1990cited by this paper

• An Overview of Contrasting Experimental Results on Dynamics of Kinesin-1 Molecular Motors: Insight into the Underlying Mechanism

  2025cites this paper
• An Engineered Cargo-Transport Molecular Motor Composed of a Kinesin Monomer and a Diffusing Microtubule-Associated Protein

  2025cites this paper
• Enhanced axonal transport in large vertebrates: KIF5A adaptations in giraffes and pythons

  2025cites this paper
• Tension-induced suppression of allosteric conformational changes coordinates kinesin-1 stepping.

  2025cites this paper
• A model of tubulin removal and exchange caused by kinesin motor walking on microtubule lattices.

  2025cites this paper
• Effects of stalk orientation and size of trapped bead on force–velocity relation of kinesin motor determined using single molecule optical trapping methods

  2025cites this paper
• Tension-induced suppression of allosteric conformational changes explains coordinated stepping of kinesin-1

  2025cites this paper
• Expanding the Molecular and Clinical Phenotype of Patients With De Novo Variants in KIF5C: A Six Patient Case Series

  2024cites this paper
• Modeling study of kinesin-13 MCAK microtubule depolymerase

  2024cites this paper
• Tether-scanning the kinesin motor domain reveals a core mechanical action

  2024cites this paper
• Modeling Studies of the Mechanism of Context-Dependent Bidirectional Movements of Kinesin-14 Motors

  2024cites this paper
• A model for cooperativity of kinesin-4 motors by communicating through the microtubule track

  2024cites this paper
• Molecular mechanism of interaction between kinesin motors affecting their residence times on microtubule lattice and end.

  2023cites this paper
• Effect of the Neck Linker on Processive Stepping of Kinesin Motor

  2023cites this paper
• Preference of CAMSAP3 for expanded microtubule lattice contributes to stabilization of the minus end

  2023cites this paper
• New insights into the mechanochemical coupling mechanism of kinesin–microtubule complexes from their high-resolution structures

  2023cites this paper
• A Frame-by-Frame Glance at Membrane Fusion Mechanisms: From Viral Infections to Fertilization

  2023cites this paper
• Increased microtubule lattice spacing correlates with selective binding of kinesin-1 in cells

  2022cites this paper
• Magic-angle-spinning NMR structure of the kinesin-1 motor domain assembled with microtubules reveals the elusive neck linker orientation

  2022cites this paper
• The molecular biology of tubulinopathies: Understanding the impact of variants on tubulin structure and microtubule regulation

  2022cites this paper
• Modeling Study of the Dynamics of Kinesin-14 Molecular Motors.

  2022cites this paper
• Multiple roles for the cytoskeleton in ALS

  2022cites this paper
• Phosphoregulation of Kinesins Involved in Long-Range Intracellular Transport

  2022cites this paper
• A neuropathy‐associated kinesin KIF1A mutation hyper‐stabilizes the motor‐neck interaction during the ATPase cycle

  2022cites this paper
• A model of processive walking and slipping of kinesin-8 molecular motors

  2021cites this paper
• Motor usage imprints microtubule stability along the shaft.

  2021cites this paper
• Molecular Mechanism of Processive Stepping of Kinesin Motors

  2021cites this paper
• Microtubule Lattice Spacing Governs Cohesive Envelope Formation of Tau Family Proteins

  2021cites this paper
• A kinesin-1 variant reveals motor-induced microtubule damage in cells

  2021cites this paper
• Dynamics of kinesin motor proteins under longitudinal and sideways loads.

  2021cites this paper
• Modeling processive motion of kinesin‐13 MCAK and kinesin‐14 Cik1‐Kar3 molecular motors

  2021cites this paper
• Studies of Conformational Changes of Tubulin Induced by Interaction with Kinesin Using Atomistic Molecular Dynamics Simulations

  2021cites this paper
• Insight into the chemomechanical coupling mechanism of kinesin molecular motors

  2021cites this paper
• Motor usage imprints microtubule stability in the shaft

  2021cites this paper
• A common ATP-dependent stepping model for kinesin-5 and kinesin-1: Mechanism of bi-directionality of kinesin-5.

  2021cites this paper
• Self-Organized Lane Formation in Bidirectional Transport by Molecular Motors.

  2020cites this paper
• Smart motors and cargo steering drive kinesin-mediated selective transport.

  2020cites this paper
• Non-tight and tight chemomechanical couplings of biomolecular motors under hindering loads.

  2020cites this paper
• Theoretical Analysis of Dynamics of Kinesin Molecular Motors

  2020cites this paper
• A common chemomechanical coupling model for orphan and conventional kinesin molecular motors.

  2020cites this paper
• How Kinesin-1 Utilize the Energy of Nucleotide: The Conformational Changes and Mechanochemical Coupling in the Unidirectional Motion of Kinesin-1

  2020cites this paper
• Stabilization of microtubules by encapsulation of the GFP using a Tau-derived peptide.

  2019cites this paper
• A Generalized Kinetic Model for Coupling between Stepping and ATP Hydrolysis of Kinesin Molecular Motors

  2019cites this paper
• Interplay between the Kinesin and Tubulin Mechanochemical Cycles Underlies Microtubule Tip Tracking by the Non-motile Ciliary Kinesin Kif7.

  2019cites this paper
• Run length distribution of dimerized kinesin-3 molecular motors: comparison with dimeric kinesin-1

  2019cites this paper
• Primary cultured neuronal networks and type 2 diabetes model mouse fatty liver tissues in aqueous liquid observed by atmospheric SEM (ASEM): Staining preferences of metal solutions.

  2019cites this paper
• Theory of microtubule length regulation in antiparallel overlaps

  2019cites this paper
• Force Dependence of Velocity and Run Length of Kinesin-1, Kinesin-2 and Kinesin-5 Family Molecular Motors

  2019cites this paper
• The model of local axon homeostasis - explaining the role and regulation of microtubule bundles in axon maintenance and pathology

  2019cites this paper
• All‐atom molecular dynamics simulations reveal how kinesin transits from one‐head‐bound to two‐heads‐bound state

  2019cites this paper
• Length regulation of microtubule antiparallel overlaps by molecular motors

  2019cites this paper
• The Spatiotemporal Construction of the Axon Initial Segment via KIF3/KAP3/TRIM46 Transport under MARK2 Signaling.

  2019cites this paper
• Force dependence of unbinding rate of kinesin motor during its processive movement on microtubule.

  2019cites this paper
• A model for the chemomechanical coupling of the mammalian cytoplasmic dynein molecular motor

  2019cites this paper
• Processivity of dimeric kinesin‐1 molecular motors

  2018cites this paper
• Structural Insights into the Altering Function of CRMP2 by Phosphorylation.

  2018cites this paper
• Structural Biology Using Electron Microscopy

  2018cites this paper
• GMPCPP-microtubule complexed with nucleotide-free KIF5C

  2018cites this paper
• Second harmonic generation polarization microscopy as a tool for protein structure analysis

  2018cites this paper
• ATP-Concentration- and Force-Dependent Chemomechanical Coupling of Kinesin Molecular Motors

  2018cites this paper
• Possible cold-adaptation for the fungal kinesin in compensation for thermal stability acquired by single amino acid substitution

  2018cites this paper
• Kinesin-binding–triggered conformation switching of microtubules contributes to polarized transport

  2018cites this paper
• Structural insight into microtubule stabilization and kinesin inhibition by Tau family MAPs

  2018cites this paper
• Investigating role of conformational changes of microtubule in regulating its binding affinity to kinesin by all‐atom molecular dynamics simulation

  2018influential citation
• Plant Structural Biology: Hormonal Regulations

  2018cites this paper
• Kinesin expands and stabilises the GDP-microtubule lattice 2 3

  2018cites this paper
• Kinesin expands and stabilises the GDP-microtubule lattice

  2018cites this paper
• Microtubule-Based Transport and the Distribution, Tethering, and Organization of Organelles.

  2017cites this paper
• Forces and Disease: Electrostatic force differences caused by mutations in kinesin motor domains can distinguish between disease-causing and non-disease-causing mutations

  2017cites this paper
• Kinesin motility is driven by subdomain dynamics

  2017influential citation
• Common general anesthetic propofol impairs kinesin processivity

  2017cites this paper
• Native kinesin‐1 does not bind preferentially to GTP‐tubulin‐rich microtubules in vitro

  2017influential citation
• Structural-mechanical remodelling of GDP-microtubules by kinesin

  2017cites this paper
• 1 1 Structural-mechanical remodelling of GDP-microtubules by kinesin 2 3

  2017cites this paper
• A model of processive movement of dimeric kinesin.

  2017cites this paper
• Acetylated Microtubules Are Preferentially Bundled Leading to Enhanced Kinesin-1 Motility.

  2017cites this paper
• Dynamics of dimeric kinesins: Limping, effect of longitudinal force, effects of neck linker extension and mutation, and comparison between kinesin-1 and kinesin-2.

  2017cites this paper
• Structural basis for CRMP2-induced axonal microtubule formation

  2017cites this paper
• Structural basis of cooperativity in kinesin revealed by 3D reconstruction of a two-head-bound state on microtubules

  2017influential citation
• Crystal structure of Zen4 in the apo state reveals a missing conformation of kinesin

  2017cites this paper
• Mechanism of Catalytic Microtubule Depolymerization via KIF2-Tubulin Transitional Conformation.

  2017cites this paper
• The cellular mechanisms that maintain neuronal polarity

  2016cites this paper
• Visualizing microtubule structural transitions and interactions with associated proteins.

  2016cites this paper
• Elastic Anisotropy Scenario for Cooperative Binding of Kinesin-Coated Beads on Microtubules.

  2016cites this paper
• structural basis of kinesin motility

  2016cites this paper
• Kinesin-2 KIF3AC and KIF3AB Can Drive Long-Range Transport along Microtubules.

  2015cites this paper
• The axonal cytoskeleton: from organization to function

  2015cites this paper