Methods of Protein Digestive Stability Assay - State of the Art

The evaluation of protein stability in digestive tract is an important topic in various fields of biomedical sciences. The most straightforward task within it is the evaluation of food quality, as during the food processing the digestibility of a protein could fall dramatically. Another task, tightly connected with the previous one, is the evaluation of protein allergenicity. This parameter is shown to be dependent on protein’s digestibility, the less digestible ones having the highest probability to induce immune response. Finally, the task of development of new peptide drugs also requires digestive stability check for per os drug forms that are the most convenient for a patient.

• Publication year

  2012

• Venue

  Unknown venue

• Publication date

  2012-04-18

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.5772/33286
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

• Effects of different processing methods on digestibility of Scylla paramamosain allergen (tropomyosin).

  2011influential reference
• Clinical immunogenicity specificity assessments: a platform evaluation.

  2011cited by this paper
• Achieving Antral Grinding Forces in Biorelevant In Vitro Models: Comparing the USP Dissolution Apparatus II and the Dynamic Gastric Model with Human In Vivo Data

  2011influential reference
• Gut permeability and food allergies

  2011influential reference
• Stability of major allergen tropomyosin and other food proteins of mud crab (Scylla serrata) by in vitro gastrointestinal digestion.

  2010influential reference
• Comparative resistance of food proteins to adult and infant in vitro digestion models.

  2010cited by this paper
• Protein digestibility evaluations of meat and fish substrates using laboratory, avian, and ileally cannulated dog assays.

  2010cited by this paper
• Immunogenicity: prediction, detection and effective assay development.

  2010cited by this paper
• In Vivo Digestion of a Thaumatin-Like Kiwifruit Protein in Rats

  2010cited by this paper
• Investigation of peptide stability upon hydrolysis by of fragments of the organs of the gastrointestinal tract of rats

  2010influential reference
• Digestibility and allergenicity assessment of enzymatically crosslinked beta-casein.

  2010influential reference
• Antioxidant Properties of Breast Milk in a Novel In Vitro Digestion/Enterocyte Model

  2010cited by this paper
• Comparison of chemical methods of protein evaluation with biological value determined on rats.

  2009influential reference
• Evaluation of cell surface-displayed protein stability against simulated gastric fluid

  2009influential reference
• In vitro digestion methods for assessing the effect of food structure on allergen breakdown.

  2009cited by this paper
• Modeling of the relationship between dipeptide structure and dipeptide stability, permeability, and ACE inhibitory activity.

  2009influential reference
• In vitro digestibility of beta-casein and beta-lactoglobulin under simulated human gastric and duodenal conditions: a multi-laboratory evaluation.

  2009cited by this paper
• Effects of gastrointestinal digestion and heating on the allergenicity of the kiwi allergens Act d 1, actinidin, and Act d 2, a thaumatin-like protein.

  2008influential reference
• Extractability, stability, and allergenicity of egg white proteins in differently heat-processed foods.

  2007cited by this paper
• β-Cryptoxanthin from Citrus juices: assessment of bioaccessibility using an in vitro digestion/Caco-2 cell culture model

  2007cited by this paper
• Human Pancreatic Digestive Enzymes

  2007cited by this paper
• Gastrointestinal digestion of food allergens: effect on their allergenicity.

  2007cited by this paper
• Beta-cryptoxanthin from citrus juices: assessment of bioaccessibility using an in vitro digestion/Caco-2 cell culture model.

  2007cited by this paper
• Moving toward a more physiological model: application of mucin to refine the in vitro digestion/Caco-2 cell culture system.

  2006cited by this paper
• Food safety: in vitro digestion tests are non-predictive for allergenic potential of food in stomach insufficiency.

  2006cited by this paper
• Digestion Assays in Allergenicity Assessment of Transgenic Proteins

  2006cited by this paper
• Phospholipid interactions protect the milk allergen alpha-lactalbumin from proteolysis during in vitro digestion.

  2005cited by this paper
• Applicability of an in vitro digestion model in assessing the bioaccessibility of mycotoxins from food.

  2005cited by this paper
• In vitro determination of the release kinetics of peptides and free amino acids during the digestion of food proteins.

  2005influential reference
• A multi-laboratory evaluation of a common in vitro pepsin digestion assay protocol used in assessing the safety of novel proteins

  2004cited by this paper
• Protein digestibility and relevance to allergenicity.

  2003cited by this paper
• Digestion Stability as a Criterion for Protein Allergenicity Assessment

  2002influential reference
• Prediction of protein digestibility in dog food by a multi-enzymatic method: a useful technique to develop.

  2001cited by this paper
• Digestibility of allergens extracted from natural rubber latex and vegetable foods.

  2000influential reference
• Quantifying the digestibility of dietary protein.

  2000cited by this paper
• Why are some proteins allergens?

  2000cited by this paper
• Molecular and cellular aspects of hydrolysis and absorption.

  1995cited by this paper
• Molecular Mass Distribution Immunological Properties Nutritive Value of Whey Protein Hydrolysates.

  1994influential reference
• The aspartic proteases.

  1992cited by this paper
• Advanced Nutrition and Human Metabolism

  1990cited by this paper
• Comparative Digestibility of Legume Storage Proteins

  1988influential reference
• Protein digestion and amino acid and peptide absorption

  1985influential reference
• Expression and intracellular transport of microvillus membrane hydrolases in human intestinal epithelial cells

  1985cited by this paper
• Enzymatic digestibility of peptides cross-linked by ionizing radiation.

  1984cited by this paper
• Experimental studies on the quality of food proteins.

  1983cited by this paper
• Limited Proteolysis of Soybean Beta-conglycinin

  1982cited by this paper
• Bioavailability: a factor in protein quality.

  1981influential reference
• Peptide hydrolases of the human small intestinal mucosa: distribution of activities between brush border membranes and cytosol.

  1980influential reference
• Peptide hydrolases of the human small intestinal mucosa: identification of six distinct enzymes in the brush border membrane.

  1980influential reference
• Digestion and absorption of dietary protein in man.

  1980cited by this paper
• Protein digestion and absorption in the rat.

  1978cited by this paper
• Digestion and absorption of protein.

  1978influential reference
• Isolation and characterization of four peptide hydrolases from the cytosol of rat intestinal mucosa.

  1977cited by this paper
• Cytoplasmic dipeptidase activities of kidney, ileum, jejunum, liver, muscle, and blood.

  1977cited by this paper
• Hydrolysis of peptides within lumen of small intestine.

  1976cited by this paper
• Intestinal phase of protein assimilation in man.

  1976influential reference
• Human Pancreatic Proteolytic Enzymes and Protein Inhibitors

  1974cited by this paper
• The pepsinogens of human gastric mucosa

  1973cited by this paper
• Peptide hydrolases in the brush border and soluble fractions of small intestinal mucosa of rat and man

  1972influential reference
• Peptide hydrolases in the bruch border and soluble fractions of small intestinal mucosa of rat and man.

  1972influential reference
• A new biological method for estimating food protein nutritive value

  1969cited by this paper
• Pepsinogens and pepsins.

  1968cited by this paper
• A comparison of biological methods with rats for determining the nutritive value of proteins

  1965cited by this paper
• Biological methods of evaluating protein quality

  1958influential reference
• Scandinavian Journal of Clinical and Laboratory Investigation

  1949cited by this paper
• The peptidases of intestinal mucosa.

  1944cited by this paper

• Bovine Milk Protein-Derived Preparations and Their Hydrolysates as Sources of ACE-Inhibitory, DPP-IV-Inhibitory, and Antioxidative Peptides Analyzed Using in Silico and in Vitro Protocols

  2025cites this paper
• Fabrication of Bletilla Striata polysaccharide-Fe (III) complex and its anti-iron deficiency anemia effects.

  2025cites this paper
• Yellow Mealworm (Tenebrio molitor) Powder Promotes a High Bioaccessible Protein Fraction and Low Glycaemic Index in Biscuits

  2023cites this paper
• Physicochemical Characterization and In Vitro Digestibility Study of an In Silico Designed Recombinant Protein Enriched with Large Neutral Amino Acids and Lacking Phenylalanine for Phenylketonuria

  2022cites this paper
• In Vitro Crude Protein Digestibility of Insects: A Review

  2022cites this paper
• Food Protein Digestomics

  2021cites this paper
• Characterization of in silico modeled synthetic protein enriched with branched-chain amino acids expressed in Pichia pastoris.

  2020cites this paper
• Characterization of egg white gel microstructure and its relationship with pepsin diffusivity

  2020cites this paper
• Proteomics in Forensic Sciences: Identification of the Nature of the Last Meal at Autopsy.

  2018cites this paper
• Guidance on allergenicity assessment of genetically modified plants

  2017cites this paper
• Protein identification and in vitro digestion of fractions from Tenebrio molitor

  2016cites this paper
• Digestive Proteases: Roles in the Human Alimentary Tract

  2016cites this paper
• A study on the potential of insect protein and lipid as a food source

  2015cites this paper
• Characteristics of Tenebrio molitor larvae and Bombyx mori pupae sequentially fermented with Aspergillus oryzae and Bacillus subtilis

  year unknowncites this paper