Both Mexico and South America are rich in Solanum species that might be valuable sources of resistance (R) genes to late blight (Phytophthora infestans). Here, we focus on an R gene present in the diploid Bolivian species S. avilesii. The genotype carrying the R gene was resistant to eight out of 10 Phytophthora isolates of various provenances. The identification of a resistant phenotype and the generation of a segregating population allowed the mapping of a single dominant R gene, Rpi-avl1, which is located in an R gene cluster on chromosome 11. This R gene cluster is considered as an R gene “hot spot”, containing R genes to at least five different pathogens. High resolution mapping of the Rpi-avl1 gene revealed a marker co-segregating in 3890 F1 individuals, which may be used for marker assisted selection in breeding programs and for further cloning of Rpi-avl1.ResumenTanto México como Sudamérica son ricos en especies de Solanum que pudieran ser valiosas fuentes de genes de resistencia (R) al tizón tardío (Phytophthora infestans). Aquí nos enfocamos en un gene R presente en la especie diploide Boliviana S. avilesii. El genotipo que contiene el gene R fue resistente a ocho de 10 aislamientos de Phytophthora de varias localidades. La identificación de un fenotipo resistente y la generación de una población segregante permitieron el mapeo de un gene R simple dominante, Rpi-av11, que se localiza en un agrupamiento de genes del cromosoma 11. Este grupo de gene R se considera como un punto clave de este gene, con un contenido de genes R para por lo menos cinco diferentes patógenos. El mapeo de alta resolución del gene Rpi-av11 reveló a un marcador co-segregando en 3890 individuos de F1, que podría usarse para selección asistida de marcadores en programas de mejoramiento y para clonaciones posteriores de Rpi-av11.
High Resolution Mapping of a Novel Late Blight Resistance Gene Rpi-avl1, from the Wild Bolivian Species Solanum avilesii
Estelle Verzaux,D. Budding,N. Vetten,R. Niks,V. Vleeshouwers,E. Vossen,E. Jacobsen,R. Visser
Published 2011 in American Journal of Potato Research
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2011
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American Journal of Potato Research
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2011-11-01
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