Transient receptor potential vanilloid 4-dependent calcium influx and ATP release in mouse and rat gastric epithelia.

AIM To explore the expression of transient receptor potential vanilloid 4 (TRPV4) and its physiological meaning in mouse and rat gastric epithelia. METHODS RT-PCR and immunochemistry were used to detect TRPV4 mRNA and protein expression in mouse stomach and a rat normal gastric epithelial cell line (RGE1-01), while Ca(2+)-imaging and electrophysiology were used to evaluate TRPV4 channel activity. ATP release was measured by a luciferin-luciferase assay. Gastric emptying was also compared between WT and TRPV4 knockout mice. RESULTS TRPV4 mRNA and protein were detected in mouse tissues and RGE1-01 cells. A TRPV4-specific agonist (GSK1016790A) increased intracellular Ca(2+) concentrations and/or evoked TRPV4-like current activities in WT mouse gastric epithelial cells and RGE1-01 cells, but not TRPV4KO cells. GSK1016790A or mechanical stimuli induced ATP release from RGE1-01 cells while TRPV4 knockout mice displayed delayed gastric emptying in vivo. CONCLUSION TRPV4 is expressed in mouse and rat gastric epithelium and contributes to ATP release and gastric emptying.

• Publication year

  2016

• Venue

  World Journal of Gastroenterology

• Publication date

  2016-06-28

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.3748/wjg.v22.i24.5512PMID 27350729PMCID PMC4917611
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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