In optical microscopy the contrast of transparent objects achieved with conventional methods is often not satisfactory, for example for the automated recognition of cells. In this paper we present a nano-optical label-free approach for contrast enhancement based on photonic crystal slabs (PCS) as the specimen holder. Quasi-guided modes inside these structures cause an intrinsic color of the PCS, which strongly depends on the wavelength and the quality factor of the optical mode. Objects on the surface of the PCS experience a significant color and intensity contrast enhancement, as they change properties of the optical modes.
Photonic crystal slabs for surface contrast enhancement in microscopy of transparent objects.
Y. Nazirizadeh,Tim Becker,Julia F. Reverey,C. Selhuber‐Unkel,D. Rapoport,U. Lemmer,M. Gerken
Published 2012 in Optics Express
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- Publication year
2012
- Venue
Optics Express
- Publication date
2012-06-18
- Fields of study
Medicine, Materials Science, Physics, Engineering
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Semantic Scholar, PubMed
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