The role of protein surface charge in catalytic activity and chloroplast membrane association of the pea NADPH: protochlorophyllide oxidoreductase (POR) as revealed by alanine scanning mutagenesis

No abstract is available for this paper.

• Publication year

  2004

• Venue

  Plant Molecular Biology

• Publication date

  Unknown publication date

• Fields of study

  Biology, Medicine, Chemistry, Environmental Science

• Identifiers
  DOI 10.1023/A:1006135100760PMID 10080697
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Protochlorophyllide photoreduction

  2004cited by this paper
• The pc-1 phenotype of Chlamydomonas reinhardtii results from a deletion mutation in the nuclear gene for NADPH:protochlorophyllide oxidoreductase

  2004cited by this paper
• Import of Proteins into Chloroplasts

  2001cited by this paper
• Chlorophyll alpha synthesis upon interruption and deletion of por coding for the light-dependent NADPH: protochlorophyllide oxidoreductase in a photosystem-I-less/chlL- strain of Synechocystis sp. PCC 6803.

  1998cited by this paper
• CHLOROPHYLL A SYNTHESIS UPON INTERRUPTION AND DELETION OF POR CODING FOR THE LIGHT-DEPENDENT NADPH:PROTOCHLOROPHYLLIDE OXIDOREDUCTASE IN A PHOTOSYSTEM -I-LESS/CHLL- STRAIN OF SYNECHOCYSTIS SP. PCC 6803

  1998cited by this paper
• Crystal structure of the ternary complex of mouse lung carbonyl reductase at 1.8 A resolution: the structural origin of coenzyme specificity in the short-chain dehydrogenase/reductase family.

  1997cited by this paper
• With chlorophyll pigments from prolamellar bodies to light‐harvesting complexes

  1997cited by this paper
• Purification and kinetic analysis of pea (Pisum sativum L.) NADPH:protochlorophyllide oxidoreductase expressed as a fusion with maltose-binding protein in Escherichia coli.

  1997cited by this paper
• The light intensity dependence of protochlorophyllide photoconversion and its significance to the catalytic mechanism of protochlorophyllide reductase

  1996cited by this paper
• Protochlorophyllide reduction: a key step in the greening of plants.

  1996cited by this paper
• Secondary structure of NADPH: protochlorophyllide oxidoreductase examined by circular dichroism and prediction methods.

  1996cited by this paper
• ABSOLUTE CONFIGURATION OF PROTOCHLOROPHYLLIDE ALPHA AND SUBSTRATE SPECIFICITY OF NADPH-PROTOCHLOROPHYLLIDE OXIDOREDUCTASE

  1996cited by this paper
• Import and routing of nucleus-encoded chloroplast proteins.

  1996cited by this paper
• The regulation of enzymes involved in chlorophyll biosynthesis.

  1996cited by this paper
• PORA and PORB, Two Light-Dependent Protochlorophyllide-Reducing Enzymes of Angiosperm Chlorophyll Biosynthesis.

  1996cited by this paper
• Comparative protein modeling by satisfaction of spatial restraints.

  1996cited by this paper
• Short-chain dehydrogenases/reductases (SDR).

  1995influential reference
• A light-dependent complementation system for analysis of NADPH:protochlorophyllide oxidoreductase: identification and mutagenesis of two conserved residues that are essential for enzyme activity.

  1995cited by this paper
• The in vitro assembly of the NADPH-protochlorophyllide oxidoreductase in pea chloroplasts

  1995cited by this paper
• Amino acids important in enzyme activity and dimer stability for Drosophila alcohol dehydrogenase.

  1995cited by this paper
• Substrate-dependent transport of the NADPH:protochlorophyllide oxidoreductase into isolated plastids.

  1995influential reference
• A light-induced protease from barley plastids degrades NADPH:protochlorophyllide oxidoreductase complexed with chlorophyllide

  1995influential reference
• Structures stabilizing the dimer interface on human 11 beta-hydroxysteroid dehydrogenase types 1 and 2 and human 15-hydroxyprostaglandin dehydrogenase and their homologs.

  1995influential reference
• Enzymatic product formation impairs both the chloroplast receptor- binding function as well as translocation competence of the NADPH: protochlorophyllide oxidoreductase, a nuclear-encoded plastid precursor protein

  1995influential reference
• Involvement of the C-terminal tail in the activity of Drosophila alcohol dehydrogenase. Evaluation of truncated proteins constructed by site-directed mutagenesis.

  1995cited by this paper
• PHOTOENZYMES : A NOVEL CLASS OF BIOLOGICAL CATALYSTS

  1994cited by this paper
• The refined three-dimensional structure of 3 alpha,20 beta-hydroxysteroid dehydrogenase and possible roles of the residues conserved in short-chain dehydrogenases.

  1994cited by this paper
• Targeting of proteins into and across the thylakoid membrane — a multitude of mechanisms

  1994cited by this paper
• Integration of nuclear-encoded proteins into pea thylakoids with different pigment contents

  1994cited by this paper
• The refined three-dimensional structure of 3α,20β-hydroxysteroid dehydrogenase and possible roles of the residues conserved in short-chain dehydrogenases

  1994influential reference
• Protochlorophyllide reductase is homologous to human carbonyl reductase and pig 20 beta-hydroxysteroid dehydrogenase.

  1994cited by this paper
• Structural comparisons lead to the definition of a new superfamily of NAD(P)(H)-accepting oxidoreductases: the single-domain reductases/epimerases/dehydrogenases (the 'RED' family).

  1994cited by this paper
• Phototransformation of monovinyl and divinyl protochlorophyllide by NADPH:protochlorophyllide oxidoreductase of barley expressed in Escherichia coli.

  1993cited by this paper
• Comparative protein modelling by satisfaction of spatial restraints.

  1993cited by this paper
• 8-vinyl reduction and chlorophyll a biosynthesis in higher plants.

  1993cited by this paper
• Carboxyethyllysine in a protein: native carbonyl reductase/NADP(+)-dependent prostaglandin dehydrogenase.

  1993cited by this paper
• Molecular cloning, nuclear gene structure, and developmental expression of NADPH: protochlorophyllide oxidoreductase in pea (Pisum sativum L.)

  1992cited by this paper
• Alanine scanning mutagenesis identifies surface amino acids on domain II of Pseudomonas exotoxin required for cytotoxicity, proper folding, and secretion into periplasm.

  1992cited by this paper
• Systematic mutational analysis of the yeast ACT1 gene.

  1992cited by this paper
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  1991cited by this paper
• PsaD is required for the stable binding of PsaC to the photosystem I core protein of Synechococcus sp. PCC 6301.

  1991cited by this paper
• Three-dimensional structure of holo 3 alpha,20 beta-hydroxysteroid dehydrogenase: a member of a short-chain dehydrogenase family.

  1991cited by this paper
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• Nucleotide sequence of a cDNA coding for the NADPH-protochlorophyllide oxidoreductase (PCR) of barley (Hordeum vulgare L.) and its expression inEscherichia coli

  1989cited by this paper
• High-resolution epitope mapping of hGH-receptor interactions by alanine-scanning mutagenesis.

  1989cited by this paper
• Protochlorophyllide reductase. 1. Determination of the regiochemistry and the stereochemistry of the reduction of protochlorophyllide to chlorophyllide

  1989cited by this paper
• Synthesis of 4R- and 4S-tritium labeled NADPH for the determination of the coenzyme stereospecificity of NADPH: protochlorophyllide oxidoreductase.

  1987cited by this paper
• Import of proteins into chloroplasts. Membrane integration of a thylakoid precursor protein reconstituted in chloroplast lysates.

  1986cited by this paper
• Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents

  1983cited by this paper
• Light-Induced Breakdown of NADPH-Protochlorophyllide Oxidoreductase In Vitro.

  1983cited by this paper
• Covalent labelling of the NADPH: protochlorophyllide oxidoreductase from etioplast membranes with [3H]N-phenylmaleimide.

  1981cited by this paper
• Purification and properties of an NADPH-dependent carbonyl reductase from human brain. Relationship to prostaglandin 9-ketoreductase and xenobiotic ketone reductase.

  1981cited by this paper
• Purification and Properties of an NADPH-dependent Carbonyl Reductase from Human Brain

  1980cited by this paper
• Substrate-specificity studies on protochlorophyllide reductase in barley (Hordeum vulgare) etioplast membranes.

  1980cited by this paper
• Reconstitution of chlorophyllide formation by isolated etioplast membranes.

  1978cited by this paper
• ACTION SPECTRA FOR BIOSYNTHESIS OF CHLOROPHYLLS a AND b AND β‐CAROTENE

  1973cited by this paper
• Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

  1970cited by this paper
• Systematic Mutational Analysis of the Yeast Actl Gene

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• Light dependent protochlorophyllide oxidoreductase: a succinct look

  2024cites this paper
• Improving alkaline stability of α-L-rhamnosidase from Aspergillus niger through computational strategy combines with folding free energy and binding free energy

  2023cites this paper
• Fortuitous events in the evolution of Light-dependent Protochlorophyllide Oxidoreductase

  2023cites this paper
• The Role of Membranes and Lipid-Protein Interactions in the Mg-Branch of Tetrapyrrole Biosynthesis

  2021cites this paper
• Crystal structures of cyanobacterial light-dependent protochlorophyllide oxidoreductase

  2020cites this paper
• Elucidation of ligand binding and dimerization of NADPH:protochlorophyllide (Pchlide) oxidoreductase from pea (Pisum sativum L.) by structural analysis and simulations

  2020cites this paper
• Enhancement in affinity of Aspergillus niger JMU-TS528 α-L-rhamnosidase (r-Rha1) by semiconservative site-directed mutagenesis of (α/α)6 catalytic domain.

  2020cites this paper
• Consensus model of a cyanobacterial light-dependent protochlorophyllide oxidoreductase in its pigment-free apo-form and photoactive ternary complex

  2019cites this paper
• Posttranslational control of tetrapyrrole biosynthesis: Interacting proteins, chaperones, auxiliary factors

  2019cites this paper
• Theoretical Model of the Protochlorophyllide Oxidoreductase from a Hierarchy of Protocols.

  2018cites this paper
• NADPH:Protochlorophyllide Oxidoreductase (POR): advanced insight into the catalytic mechanism of the two plant isozymes POR A and POR B

  2018cites this paper
• Multiple active site residues are important for photochemical efficiency in the light-activated enzyme protochlorophyllide oxidoreductase (POR)

  2016cites this paper
• Modulation of Chlorophyll Biosynthesis by Environmental Cues

  2013cites this paper
• Chlorophyll Biosynthesis Gene Evolution Indicates Photosystem Gene Duplication, Not Photosystem Merger, at the Origin of Oxygenic Photosynthesis

  2012cites this paper
• Chlorophyll Biosynthesis in Higher Plants

  2012cites this paper
• Light-dependent and light-independent protochlorophyllide oxidoreductases share similar sequence motifs —in silico studies

  2012cites this paper
• Assembly of NADPH: protochlorophyllide oxidoreductase complex is needed for effective greening of barley seedlings.

  2012cites this paper
• Characterization of ovine Toll-like receptor 9 protein coding region, comparative analysis, detection of mutations and maedi visna infection.

  2011cites this paper
• Chlorophyll biosynthesis: spotlight on protochlorophyllide reduction.

  2010cites this paper
• Action Spectra of Chlorophyll a Biosynthesis in Cyanobacteria: Dark-Operative Protochlorophyllide Oxidoreductase-Deficient Mutants

  2009cites this paper
• Photoprotective role of NADPH:protochlorophyllide oxidoreductase A

  2008cites this paper
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  2008cites this paper
• New Perspectives in Biological Nitrogen Fixation Research

  2008cites this paper
• Photoactive pigment—enzyme complexes of chlorophyll precursor in plant leaves

  2007cites this paper
• In vitro-mutagenesis of NADPH:protochlorophyllide oxidoreductase B: two distinctive protochlorophyllide binding sites participate in enzyme catalysis and assembly

  2006cites this paper
• Advances in Photosynthesis and Respiration: Focus on Plant Respiration

  2005cites this paper
• Organization of protochlorophyllide oxidoreductase in prolamellar bodies isolated from etiolated carotenoid-deficient wheat leaves as revealed by fluorescence probes.

  2005cites this paper
• Novel Insights into the Enzymology, Regulation and Physiological Functions of Light-dependent Protochlorophyllide Oxidoreductase in Angiosperms

  2004influential citation
• POR hits the road: import and assembly of a plastid protein

  2004cites this paper
• Hg(2+) reacts with different components of the NADPH : protochlorophyllide oxidoreductase macrodomains.

  2004cites this paper
• Formation of the Pigment Apparatus in Etiolated Barley Leaves under the Influence of Levulinic Acid

  2004cites this paper
• Origin and evolution of the light-dependent protochlorophyllide oxidoreductase (LPOR) genes.

  2004cites this paper
• Origin and Evolution of the Light-Dependent Protochlorophyllide Oxidoreductase (LPOR) Genes

  2004influential citation
• Hg2+ Reacts with Different Components of the NADPH : Protochlorophyllide Oxidoreductase Macrodomains

  2004cites this paper
• POR structural domains important for the enzyme activity in R. capsulatus complementation system

  2004cites this paper
• The Extra Loop Distinguishing POR from the Structurally Related Short-chain Alcohol Dehydrogenases Is Dispensable for Pigment Binding but Needed for the Assembly of Light-harvesting POR-Protochlorophyllide Complex*

  2003cites this paper
• Protochlorophyllide Reduction: Mechanisms and Evolution ¶

  2003cites this paper
• Biosynthesis of chlorophylls from protoporphyrin IX.

  2003cites this paper
• Protochlorophyllide reduction: mechanisms and evolutions.

  2003cites this paper
• Protochlorophyllide Reduction: Mechanisms and Evolution¶

  2003cites this paper
• POR - import and membrane association of a key element in chloroplast development.

  2003cites this paper
• The plant S-adenosyl-L-methionine:Mg-protoporphyrin IX methyltransferase is located in both envelope and thylakoid chloroplast membranes.

  2002cites this paper
• The importance of the C‐terminal region and Cys residues for the membrane association of the NADPH:protochlorophyllide oxidoreductase in pea

  2001cites this paper
• Tyr275 and Lys279 stabilize NADPH within the catalytic site of NADPH:protochlorophyllide oxidoreductase and are involved in the formation of the enzyme photoactive state.

  2001cites this paper
• Association of the NADPH:protochlorophyllide oxidoreductase (POR) with isolated etioplast inner membranes from wheat.

  2001cites this paper
• Protochlorophyllide oxidoreductase B-catalyzed protochlorophyllide photoreduction in vitro: insight into the mechanism of chlorophyll formation in light-adapted plants.

  1999cites this paper