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Screening miRNAs associated with resistance gemcitabine from exosomes in A549 lung cancer cells

Ai-Ling Liang,Shen-Lin Du,Bin Zhang,Jing Zhang,Xuan Ma,C. Wu,Yong-Jun Liu

Published 2019 in Cancer Management and Research

ABSTRACT

Purpose To establish a gemcitabine-resistant lung adenocarcinoma cell line, A549/G+, and to screen the differences of miRNA expression in exosomes from A549 and A549/G+ cells. Methods A549 cells were exposed in gemcitabine until they were resistant to gemcitabine, and extracted exosomes from A549 and A549/G+. The RNAs from exosomes were subjected to miRNA expression microarray experiments. Results After 39 weeks of continuous induction, we induced drug resistance in A549 cells. The resistance index was 6. Via GeneChip miRNA 4.0 analysis, there were 446 differential miRNAs between A549 and A549/G+. Target gene prediction and pathway analysis discovered the microRNAs in the intersections may participate in drug resistance. Conclusion These differential miRNAs help to do in-depth research to elucidate the mechanism of resistance to gemcitabine in non-small cell lung cancer.

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