Resident CAPS on dense-core vesicles docks and primes vesicles for fusion

The exocytosis of dense-core vesicles requires a priming step during which SNARE protein complexes assemble. CAPS, which assembles SNARE complexes and is required for vesicle priming, was imaged during events of single-vesicle fusion by TIRF microscopy. CAPS was found to be a resident on vesicles, with residence required for docking and fusion.

• Publication year

  2016

• Venue

  Molecular Biology of the Cell

• Publication date

  2016-02-15

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1091/mbc.E15-07-0509PMID 26700319PMCID 4750925
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Correction: CAPS-1 promotes fusion competence of stationary dense-core vesicles in presynaptic terminals of mammalian neurons

  2015cited by this paper
• CAPS-1 promotes fusion competence of stationary dense-core vesicles in presynaptic terminals of mammalian neurons

  2015influential reference
• Every laboratory with a fluorescence microscope should consider counting molecules

  2014cited by this paper
• Systematic spatial mapping of proteins at exocytic and endocytic structures

  2014cited by this paper
• Contact-induced clustering of syntaxin and munc18 docks secretory granules at the exocytosis site

  2014cited by this paper
• CAPS and Munc13 utilize distinct PIP2-linked mechanisms to promote vesicle exocytosis

  2014influential reference
• Tethering the assembly of SNARE complexes.

  2014cited by this paper
• CAPS and Munc13: CATCHRs that SNARE Vesicles

  2013influential reference
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  2013cited by this paper
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  2013cited by this paper
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  2013influential reference
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  2013cited by this paper
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  2012influential reference
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  2012influential reference
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  2012influential reference
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  2012cited by this paper
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  2012cited by this paper
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  2012cited by this paper
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  2011influential reference
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  2010cited by this paper
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  2010cited by this paper
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  2010cited by this paper
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  2010cited by this paper
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  2010cited by this paper
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  2010cited by this paper
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  2010cited by this paper
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  2010cited by this paper
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  2010cited by this paper
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  2010cited by this paper
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  2009influential reference
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  2009cited by this paper
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  2009cited by this paper
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  2009cited by this paper
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  2009influential reference
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  2008cited by this paper
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  2008cited by this paper
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  2008influential reference
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  2008cited by this paper
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  2008cited by this paper
• Vesicle Docking in Regulated Exocytosis

  2008cited by this paper
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  2008influential reference
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  2008influential reference
• CAPS Facilitates Filling of the Rapidly Releasable Pool of Large Dense-Core Vesicles

  2008influential reference
• CAPS-1 and CAPS-2 are essential synaptic vesicle priming proteins.

  2007cited by this paper
• Munc18-1 is critical for plasma membrane localization of syntaxin1 but not of SNAP-25 in PC12 cells.

  2007cited by this paper
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  2007cited by this paper
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  2007cited by this paper
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  2007cited by this paper
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  2007influential reference
• Ca2+-dependent Activator Protein for Secretion 1 Is Critical for Constitutive and Regulated Exocytosis but Not for Loading of Transmitters into Dense Core Vesicles*

  2007influential reference
• Regulation of membrane fusion in synaptic excitation-secretion coupling: speed and accuracy matter.

  2007influential reference
• PKA activation bypasses the requirement for UNC-31 in the docking of dense core vesicles from C. elegans neurons.

  2007cited by this paper
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  2006cited by this paper
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  2006cited by this paper
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  2006cited by this paper
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  2006cited by this paper
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  2006influential reference
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  2006cited by this paper
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  2006influential reference
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  2005cited by this paper
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  2005cited by this paper
• plasma membrane

  2005cited by this paper
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  2005cited by this paper
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  2004cited by this paper
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  2004cited by this paper
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  2004cited by this paper
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  2003cited by this paper
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  2003cited by this paper
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  2003cited by this paper
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  2002cited by this paper
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  2002influential reference
• Drosophila CAPS is an essential gene that regulates dense-core vesicle release and synaptic vesicle fusion.

  2001cited by this paper
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  2001cited by this paper
• Multifaceted physiological response allows yeast to adapt to the loss of the signal recognition particle-dependent protein-targeting pathway.

  2001cited by this paper
• Role of actin cortex in the subplasmalemmal transport of secretory granules in PC-12 cells.

  2000influential reference
• SNAREpins: minimal machinery for membrane fusion.

  1998cited by this paper
• CAPS (mammalian UNC-31) protein localizes to membranes involved in dense-core vesicle exocytosis.

  1998influential reference
• Multiple kinetic components of exocytosis distinguished by neurotoxin sensitivity

  1998cited by this paper
• Novel Ca2+-binding Protein (CAPS) Related to UNC-31 Required for Ca2+-activated Exocytosis*

  1997cited by this paper
• Docked Secretory Vesicles Undergo Ca2+-activated Exocytosis in a Cell-free System*

  1997cited by this paper
• SNAP receptors implicated in vesicle targeting and fusion

  1993cited by this paper
• A novel 145 kd brain cytosolic protein reconstitutes Ca(2+)-regulated secretion in permeable neuroendocrine cells.

  1992cited by this paper

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  2024cites this paper
• Vesicle docking and fusion pore modulation by the neuronal calcium sensor Synaptotagmin-1

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  2023cites this paper
• The C2 and PH domains of CAPS constitute an effective PI(4,5)P2-binding unit essential for Ca2+-regulated exocytosis.

  2023cites this paper
• Synaptic Secretion and Beyond: Targeting Synapse and Neurotransmitters to Treat Neurodegenerative Diseases

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• The Polarized Redistribution of the Contractile Vacuole to the Rear of the Cell is Critical for Streaming and is Regulated by PI(4,5)P2-Mediated Exocytosis

  2022cites this paper
• Phosphoproteome reveals molecular mechanisms of aberrant rhythm in neurotransmitter‐mediated islet hormone secretion in diabetic mice

  2022cites this paper
• Rabphilin 3A binds the N-peptide of SNAP-25 to promote SNARE complex assembly in exocytosis

  2022cites this paper
• CADPS functional mutations in patients with bipolar disorder increase the sensitivity to stress

  2022cites this paper
• Phorbolester-activated Munc13-1 and ubMunc13-2 exert opposing effects on dense-core vesicle secretion

  2022cites this paper
• Hypodermal ribosome synthesis inhibition induces a nutrition-uncoupled organism-wide growth quiescence in C. elegans

  2022cites this paper
• A unique C2 domain at the C terminus of Munc13 promotes synaptic vesicle priming

  2021cites this paper
• CAPS2 Deficiency Impairs the Release of the Social Peptide Oxytocin, as Well as Oxytocin-Associated Social Behavior

  2021cites this paper
• CAPS1 is involved in hippocampal synaptic plasticity and hippocampus-associated learning

  2021cites this paper
• Munc18-dependent and -independent clustering of syntaxin in the plasma membrane of cultured endocrine cells

  2021cites this paper
• Journey of brain-derived neurotrophic factor: from intracellular trafficking to secretion

  2020cites this paper
• Phosphatidylinositol(4,5)bisphosphate: diverse functions at the plasma membrane

  2020cites this paper
• CK2 Phosphorylation Is Required for Regulation of Syntaxin 1A Activity in Ca2+-Triggered Release in Neuroendocrine Cells

  2020influential citation
• Structural and Functional Analysis of the CAPS SNARE-Binding Domain Required for SNARE Complex Formation and Exocytosis.

  2019cites this paper
• In vitro fusion of single synaptic and dense core vesicles reproduces key physiological properties

  2019cites this paper
• The priming factor CAPS1 regulates dense-core vesicle acidification by interacting with rabconnectin3β/WDR7 in neuroendocrine cells

  2019influential citation
• Control of insulin granule formation and function by the ABC transporters ABCG1 and ABCA1 and by oxysterol binding protein OSBP

  2018cites this paper
• An Alternative Exon of CAPS2 Influences Catecholamine Loading into LDCVs of Chromaffin Cells

  2018cites this paper
• High Throughput NPY-Venus and Serotonin Secretion Assays for Regulated Exocytosis in Neuroendocrine Cells.

  2018cites this paper
• (2018). Reconstruction of the ancestral metazoan genome reveals an increase in genomic novelty. Nature Communications

  2018cites this paper
• Reconstruction of the ancestral metazoan genome reveals an increase in genomic novelty

  2018cites this paper
• Active Zone Material-Directed Orientation, Docking, and Fusion of Dense Core Vesicles Alongside Synaptic Vesicles at Neuromuscular Junctions

  2018cites this paper
• Doc2B acts as a calcium sensor for vesicle priming requiring synaptotagmin-1, Munc13-2 and SNAREs

  2017cites this paper
• Phosphatidylinositol 4,5-bisphosphate optical uncaging potentiates exocytosis

  2017cites this paper
• Reconstitution of calcium-mediated exocytosis of dense-core vesicles

  2017influential citation
• The Dual Function of the Polybasic Juxtamembrane Region of Syntaxin 1A in Clamping Spontaneous Release and Stimulating Ca2+-Triggered Release in Neuroendocrine Cells

  2017cites this paper
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  2017cites this paper
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  2016influential citation
• Neuropeptidergic control of synaptic vesicle filling and behavior in the nematode "Caenorhabditis elegans"

  2016cites this paper