Leishmania in synanthropic rodents (Rattus rattus): new evidence for the urbanization of Leishmania (Leishmania) amazonensis.

This study aimed to detect parasites from Leishmania genus, to determine the prevalence of anti-Leishmania spp. antibodies, to identify circulating species of the parasite, and to determine epidemiological variables associated with infection in rats caught in urban area of Londrina, Paraná, Brazil. Animal capture was carried out from May to December 2006, serological and molecular methods were performed. DNA was extracted from total blood, and nested-PCR, targeting SSu rRNA from Leishmania genus, was performed in triplicate. The positive samples were sequenced twice by Sanger method to species determination. In total, 181 rodents were captured, all were identified as Rattus rattus and none showed clinical alterations. Forty-one of the 176 (23.3%) animals were positive for Leishmania by ELISA and 6/181 (3.3%) were positive by IFAT. Nine of 127 tested animals (7.1%) were positive by PCR; seven were identified as L. (L.) amazonensis, one as L. (L.) infantum. Four rats were positive using more than one test. This was the first description of synanthropic rodents naturally infected by L. (L.) amazonensis (in the world) and by L. (L.) infantum (in South Brazil). Regarding L. (L.) amazonensis, this finding provides new evidence of the urbanization of this etiological agent.

• Publication year

  2017

• Venue

  Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria

• Publication date

  Unknown publication date

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1590/S1984-29612017001PMID 28177041
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• LEISHMANIOSE TEGUMENTAR AMERICANA

  2018cited by this paper
• Leishmaniasis in dogs from recycling centers and from a neighborhood with adjacent forest in an urban area of Londrina, Paraná, Brazil

  2016influential reference
• Ecological Niche Modelling Predicts Southward Expansion of Lutzomyia (Nyssomyia) flaviscutellata (Diptera: Psychodidae: Phlebotominae), Vector of Leishmania (Leishmania) amazonensis in South America, under Climate Change

  2015cited by this paper
• Infectiousness of Sylvatic and Synanthropic Small Rodents Implicates a Multi-host Reservoir of Leishmania (Viannia) braziliensis

  2015cited by this paper
• Leishmania infantum in wild rodents: reservoirs or just irrelevant incidental hosts?

  2015cited by this paper
• Species Typing in Dermal Leishmaniasis

  2015cited by this paper
• Expanding the knowledge about Leishmania species in wild mammals and dogs in the Brazilian savannah

  2015cited by this paper
• IDENTIFICATION OF SANDFLIES (Diptera: Psychodidae: Phlebotominae) BLOOD MEALS IN AN ENDEMIC LEISHMANIASIS AREA IN BRAZIL

  2015cited by this paper
• Natural infection of phlebotomines (Diptera: Psychodidae) by Leishmania (Leishmania) amazonensis in an area of ecotourism in Central-Western Brazil

  2015cited by this paper
• The shadows of a ghost: a survey of canine leishmaniasis in Presidente Prudente and its spatial dispersion in the western region of São Paulo state, an emerging focus of visceral leishmaniasis in Brazil

  2015cited by this paper
• Illegal Waste Sites As A Potential Micro Foci Of Mediterranean Leishmaniasis: First Records Of Phlebotomine Sand Flies (Diptera: Psychodidae) From Slovenia

  2015influential reference
• Mixed infection of Leishmania infantum and Leishmania braziliensis in rodents from endemic urban area of the New World

  2015cited by this paper
• Molecular detection of Leishmania spp. in road-killed wild mammals in the Central Western area of the State of São Paulo, Brazil

  2014cited by this paper
• Wild and synanthropic reservoirs of Leishmania species in the Americas

  2014cited by this paper
• Native Rodent Species Are Unlikely Sources of Infection for Leishmania (Viannia) braziliensis along the Transoceanic Highway in Madre de Dios, Peru

  2014cited by this paper
• Molecular Survey on Detection of Leishmania Infection in Rodent Reservoirs in Jahrom District, Southern Iran

  2014cited by this paper
• Seroepidemiology of Leishmania spp. in dogs residing in Telêmaco Borba, Paraná, Brazil

  2014cited by this paper
• Rattus norvegicus (Rodentia: Muridae) Infected by Leishmania (Leishmania) infantum (syn. Le. chagasi) in Brazil

  2014cited by this paper
• Distinct Leishmania Species Infecting Wild Caviomorph Rodents (Rodentia: Hystricognathi) from Brazil

  2014cited by this paper
• Diversity of Leishmania species and of strains of Leishmania major isolated from desert rodents in different foci of cutaneous leishmaniasis in Iran.

  2014cited by this paper
• ARE RODENTS A POTENTIAL RESERVOIR FOR LEISHMANIA INFANTUM IN ITALY

  2014cited by this paper
• High rates of Leishmania infantum and Trypanosoma nabiasi infection in wild rabbits (Oryctolagus cuniculus) in sympatric and syntrophic conditions in an endemic canine leishmaniasis area: epidemiological consequences.

  2014cited by this paper
• Epidemiology of Leishmania infantum, Toxoplasma gondii, and Neospora caninum in Rattus rattus in absence of domestic reservoir and definitive hosts.

  2014cited by this paper
• Small mammals as hosts of Leishmania spp. in a highly endemic area for zoonotic leishmaniasis in North-Eastern Brazil.

  2013cited by this paper
• American cutaneous leishmaniasis: epidemiological profile of patients treated in Londrina from 1998 to 2009

  2013cited by this paper
• Blood feeding patterns of Nyssomyia intermedia and Nyssomyia neivai (Diptera, Psychodidae) in a cutaneous leishmaniasis endemic area of the Ribeira Valley, State of São Paulo, Brazil.

  2013cited by this paper
• Sandflies (Diptera: Psychodidae) in rural and urban environments in an endemic area of cutaneous leishmaniasis in southern Brazil.

  2013cited by this paper
• Leishmaniasis transmission in an ecotourism area: potential vectors in Ilha Grande, Rio de Janeiro State, Brazil

  2013cited by this paper
• Divergent Profile of Emerging Cutaneous Leishmaniasis in Subtropical Brazil: New Endemic Areas in the Southern Frontier

  2013cited by this paper
• Detection of DNA from Leishmania (Viannia): Accuracy of Polymerase Chain Reaction for the Diagnosis of Cutaneous Leishmaniasis

  2013cited by this paper
• Natural infection of synathropic rodent species Mus musculus and Rattus norvegicus by Leishmania infantum in Sesimbra and Sintra – Portugal

  2013cited by this paper
• Animal Reservoirs of Visceral Leishmaniasis in India

  2013cited by this paper
• PHLEBOTOMINE SANDFLIES IN RURAL LOCATIONS IN THE STATE OF PARANA, SOUTHERN BRAZIL

  2013cited by this paper
• Ecological aspects of the sandfly fauna (Diptera, Psychodidae) in an American cutaneous leishmaniasis endemic area under the influence of hydroelectric plants in Paranapanema river, State of Paraná, Brazil.

  2012cited by this paper
• Environmental and Animal Characteristics as Factors Associated with American Cutaneous Leishmaniasis in Rural Locations with Presence of Dogs, Brazil

  2012cited by this paper
• Leishmaniasis Worldwide and Global Estimates of Its Incidence

  2012cited by this paper
• Leishmania amazonensis em cão com quadro clínico de leishmaniose visceral no Estado do Paraná, Brasil - relato de caso Leishmania amazonensis in dog with clinical diagnosis of visceral leishmaniasis in Paraná State, Brazil - a case report

  2012cited by this paper
• The first record of Lutzomyia longipalpis (Lutz & Neiva, 1912) (Diptera: Psychodidae: Phlebotominae) in the State of Paraná, Brazil.

  2012cited by this paper
• Soroprevalência da Leishmaniose Tegumentar Americana (LTA) canina e fauna de Flebotomíneos (Diptera: Psychodidae) em Bela Vista do Paraíso, Paraná

  2011cited by this paper
• Eco-epidemiology of visceral leishmaniasis in the urban area of Paracatu, state of Minas Gerais, Brazil.

  2011cited by this paper
• Molecular Screening of Wild Rodents for Leishmania infantum in Germany

  2011cited by this paper
• Recent advances in leishmaniosis in pet animals: epidemiology, diagnostics and anti-vectorial prophylaxis.

  2011cited by this paper
• Molecular detection of Leishmania braziliensis in Rattus norvegicus in an area endemic for cutaneous leishmaniasis in Brazil.

  2011cited by this paper
• Wild, synanthropic and domestic hosts of Leishmania in an endemic area of cutaneous leishmaniasis in Minas Gerais State, Brazil.

  2011influential reference
• Molecular Detection of Leishmania infantum in Wild Rodents (Rattus norvegicus) in Greece

  2010cited by this paper
• Characterization of Leishmania spp. causing cutaneous leishmaniasis in Manaus, Amazonas, Brazil

  2010cited by this paper
• Leishmania species: detection and identification by nested PCR assay from skin samples of rodent reservoirs.

  2010cited by this paper
• Sand fly captures with Disney traps in area of occurrence of Leishmania (Leishmania) amazonensis in the state of Mato Grosso do Sul, mid-western Brazil.

  2010cited by this paper
• Cutaneous and visceral leishmaniasis in dogs from a rural community in northeastern Brazil.

  2010cited by this paper
• Sand flies (Diptera: Psychodidae) in the urban area of the municipality of Cianorte, Paraná State, Brazil.

  2009cited by this paper
• CONSIDERAÇÕES SOBRE A DISPERSÃO DA LEISHMANIOSE TEGUMENTAR AMERICANA NAS AMÉRICAS

  2009influential reference
• [Diversity, distribution and abundance of sandflies (Diptera: Psychodidae) in Parana State, Southern Brazil].

  2008cited by this paper
• A study of cross-reactivity in serum samples from dogs positive for Leishmania sp., Babesia canis and Ehrlichia canis in enzyme-linked immunosorbent assay and indirect fluorescent antibody test.

  2008cited by this paper
• Spread of Vector-borne Diseases and Neglect of Leishmaniasis, Europe

  2008cited by this paper
• Diversidade, distribuição e abundância de flebotomíneos (Diptera: Psychodidae) no Paraná

  2008cited by this paper
• Guia dos roedores do Brasil, com chaves para gêneros baseadas em caracteres externos

  2008cited by this paper
• Kinetoplastid Biology and Disease BioMed Central

  2007cited by this paper
• Leishmania (Viannia) braziliensis: epidemiology of canine cutaneous leishmaniasis in the State of Paraná (Brazil).

  2007cited by this paper
• The first records of Leishmania (Leishmania) amazonensis in dogs (Canis familiaris) diagnosed clinically as having canine visceral leishmaniasis from Araçatuba County, São Paulo State, Brazil.

  2007cited by this paper
• [Preliminary study on feeding preference of Nyssomyia neivai (Pinto) and Nyssomyia whitmani (Antunes & Coutinho) (Diptera: Psychodidae) in a rural area of the State of Paraná, South Brazil].

  2007cited by this paper
• Modification of Disney trap for capture of sand flies (Diptera: Psychodidae: Phlebotominae).

  2007cited by this paper
• Ecology of the leishmania species in the Guianan ecoregion complex.

  2006cited by this paper
• [Occurrence of American tegumentary leishmaniasis in the Mato Grosso do Sul State associated to the infection for Leishmania (Leishmania) amazonensis].

  2006cited by this paper
• Ocorrência de leishmaniose tegumentar americana no Estado do Mato Grosso do Sul associada à infecção por Leishmania (Leishmania) amazonensis

  2006cited by this paper
• Manual de vigilância e controle da leishmaniose visceral americana do Estado de São Paulo

  2006cited by this paper
• Estudo sobre a participação de roedores na cadeia de transmissão de Leishmania infantum (Protozoa: Trypanosomatidae) no Rio Grande do Norte

  2005cited by this paper
• Feline leishmaniasis due to Leishmania (Leishmania) amazonensis in Mato Grosso do Sul State, Brazil.

  2005cited by this paper
• Comparação de métodos diagnósticos em leishmaniose tegumentar americana

  2005cited by this paper
• PCR-based diagnosis for detection of Leishmania in skin and blood of rodents from an endemic area of cutaneous and visceral leishmaniasis in Brazil.

  2005influential reference
• Flebotomíneos de municípios do norte do estado do Paraná, sul do Brasil

  2004cited by this paper
• Leishmania tropica in the black rat (Rattus rattus): persistence and transmission from asymptomatic host to sand fly vector Phlebotomus sergenti.

  2003cited by this paper
• Wild and synanthropic hosts of Leishmania (Viannia) braziliensis in the endemic cutaneous leishmaniasis locality of Amaraji, Pernambuco State, Brazil.

  2003cited by this paper
• Cotton rats (Sigmodon hispidus) and black rats (Rattus rattus) as possible reservoirs of Leishmania spp. in Lara State, Venezuela.

  2002cited by this paper
• Detection of antibodies to Leishmania donovani in animals in a kala-azar endemic region in eastern Sudan: a preliminary report.

  2000cited by this paper
• Putative reservoirs of Leishmania amazonensis in a sub-andean focus of Bolivia identified by kDNA-polymerase chain reaction.

  1999cited by this paper
• Epidemiologic aspects of American visceral leishmaniasis in an endemic focus in Eastern Venezuela.

  1999cited by this paper
• Environmental sanitation and peri-domiciliar organisation as auxiliary practices for the control of phlebotomines in Paraná state, southern Brazil

  1999cited by this paper
• Detection of Leishmania (Viannia) braziliensis complex in wild mammals from Colombian coffee plantations by PCR and DNA hybridization.

  1998cited by this paper
• Leishmaniose tegumentar americana: uso de técnicas da biologia molecular no diagnóstico de infecçäo de roedores da coleçäo do Museu Nacional - UFRJ

  1998cited by this paper
• Rapid identification of Leishmania species from formalin-fixed biopsy samples by polymorphism-specific polymerase chain reaction.

  1998cited by this paper
• Leishmaniasis reservoirs and their significance in control.

  1996cited by this paper
• Flebotomíneos em área de transmissão de leishmaniose tegumentar na região norte do Estado do Paraná-Brasil: Variação Sazonal e Atividade Noturna

  1993cited by this paper
• [Phlebotomines in an area of transmission of tegumentar leishmaniasis in the northern region of Parana State, Brazil: seasonal variation and nocturnal activity].

  1993cited by this paper
• Sequence analysis of small subunit ribosomal RNA genes and its use for detection and identification of Leishmania parasites.

  1992cited by this paper
• Leishmania infecting man and wild animals in Saudi Arabia. 9. The black rat (Rattus rattus) a probable reservoir of visceral leishmaniasis in Gizan province, south-west Saudi Arabia.

  1992cited by this paper
• Leishmaniasis in Bahia, Brazil: evidence that Leishmania amazonensis produces a wide spectrum of clinical disease.

  1991influential reference
• Epi Info: a general-purpose microcomputer program for public health information systems.

  1991influential reference
• An autochthonous case of cutaneous leishmaniasis caused by Leishmania (Leishmania) amazonensis Lainson & Shaw, 1972 from the north of Paraná State, Brazil.

  1990cited by this paper
• Lutzomyia reducta Feliciangeli et al., 1988, a host of Leishmania amazonensis, sympatric with two other members of the Flaviscutellata complex in southern Amazonas and Rondônia, Brazil (Diptera: Psychodidae)

  1989cited by this paper
• Anti-leishmanial antibodies during natural infection of Psammomys obesus and Meriones shawi (Rodentia, Gerbillinae) by Leishmania major.

  1989cited by this paper
• Leishmania infecting man and wild animals in Saudi Arabia. 6. Cutaneous leishmaniasis of man in the south-west.

  1989cited by this paper
• Leishmaniasis in Tuscany (Italy) V. Further isolation of Leishmania from Rattus rattus in the Province of Grosseto.

  1981cited by this paper
• [Phlebotominae from Londrina, Paraná (Brazil) and ecological observations of some species].

  1977cited by this paper
• Phlebotominae from Londrina, State of Paraná, Brazil, and ecological observations on some species

  1977influential reference
• Isolation of Leishmania from a black rat in the Baghdad area, Iraq.

  1976influential reference
• Leishmaniasis in Brazil. VI. Observations on the seasonal variations of Lutzomyia flaviscutellata in different types of forest and its relationship to enzootic rodent leishmaniasis (Leishmania mexicana amazonensis).

  1972cited by this paper
• Leishmaniasis in Brazil. V. Studies on the epidemiology of cutaneous leishmaniasis in Mato Grosso State, and observations on two distinct strains of Leishmania isolated from man and forest animls.

  1970cited by this paper
• Leishmaniasis in Brazil: I. Observations on enzootic rodent leishmaniasis--incrimination of Lutzomyia flaviscutellata (Mangabeira) as the vector in the Lower Amazonian Basin.

  1968cited by this paper

• Molecular Detection of Trypanosomatids in Rodents and Marsupials in the State of Amapá, Brazil

  2025cites this paper
• Detection of Leishmania spp. in Small Non-Flying Mammals (Didelphimorphia and Rodentia) from Bahia, Northeast Brazil

  2025cites this paper
• Synanthropic Rodents as Bioindicator of Human Pathogens in a Tourist Area of Brazil

  2025cites this paper
• Bioecological aspects and integrative taxonomy of sand flies (Diptera: Psychodidae: Phlebotomina)

  2024cites this paper
• Leishmania major Infection in Synanthropic Rodents: Evidence for the Urbanization of Zoonotic Cutaneous Leishmaniasis (ZCL) in Southern Iran

  2024cites this paper
• Genetic characterization and description of Leishmania (Leishmania) ellisi sp. nov.: a new human-infecting species from the USA

  2023cites this paper
• Zinc and Manganese Imbalances in BALB/c Mice Experimentally Infected with Leishmania (Leishmania) amazonensis

  2023cites this paper
• Calomyscid Rodents (Rodentia: Calomyscidae) as a Potential Reservoir of Zoonotic Cutaneous Leishmaniasis in a Mountainous Residential Area in the Plateau of Iran: Inferring from Molecular Data of kDNA and ITS2 Genes of Leishmania Major

  2023cites this paper
• Identification of infection by Leishmania spp. in wild and domestic animals in Brazil: a systematic review with meta-analysis (2001–2021)

  2023cites this paper
• Leishmania species infection of bats: A systematic review.

  2023cites this paper
• Leishmania en piel de Rattus rattus de zona urbana de la Ciudad de Corrientes, Argentina

  2022cites this paper
• Phlebotomine Fauna (Diptera: Psychodidae) and Infection by Leishmania spp. in Forest Fragments of a University Campus, Western Amazon

  2022cites this paper
• The Presence of Mycobacterium leprae in Wild Rodents

  2022cites this paper
• Predominance of Leishmania (Leishmania) amazonensis DNA in Lutzomyia longipalpis sand flies (Diptera: Psychodidae) from an endemic area for leishmaniasis in Northeastern Brazil

  2022cites this paper
• Investigation of Leishmania (Viannia) braziliensis Infection in Wild Mammals in Brazil

  2022cites this paper
• Experimental transmission of Leishmania (Leishmania) amazonensis to immunosuppressed mice through the bite of Lutzomyia longipalpis (Diptera: Psychodidae) results in cutaneous leishmaniasis

  2021cites this paper
• American Tegumentary Leishmaniasis in an endemic municipality in the North of Minas Gerais State: spatial analysis and socio-environmental factors

  2021cites this paper
• Epidemiological and genetic characteristics of asymptomatic canine leishmaniasis and implications for human Leishmania infections in Egypt

  2021cites this paper
• Life-histories as determinants of infection prevalence for trypanosomatids: A meta-analysis

  2021cites this paper
• Evaluation of an active and early surveillance methodology for visceral leishmaniasis by molecular detection in road-killed wild fauna.

  2021cites this paper
• Presence of trypanosomatids, with emphasis on Leishmania, in Rodentia and Didelphimorphia mammals of a rural settlement in the central Amazon region

  2021cites this paper
• Leishmaniasis: an overview of evolution, classification, distribution, and historical aspects of parasite and its vector

  2021cites this paper
• Trypanosomatid Richness in Wild and Synanthropic Small Mammals from a Biological Station in Rio de Janeiro, Brazil

  2021cites this paper
• American cutaneous leishmaniasis associated with degradation of native forest, regardless of economic, social and infrastructure vulnerability

  2020cites this paper
• Survey of anti-Toxoplasma gondii antibodies in wild mammals captured from Atlantic Forest fragments in Bahia, northeastern Brazil.

  2020cites this paper
• Duplex qPCR for Leishmania species identification using lesion imprint on filter paper.

  2020cites this paper
• Composition and Vertical Stratification of Phlebotomine Sand Fly Fauna and the Molecular Detection of Leishmania in Forested Areas in Rondônia State Municipalities, Western Amazon, Brazil

  2019cites this paper
• First Molecular Report of Leishmania (Leishmania) amazonensis and Leishmania (Viannia) guyanensis in Paraguayan Inhabitants Using High-Resolution Melt-PCR.

  2019cites this paper
• Canine Leishmaniasis: An Overview of the Current Status and Strategies for Control

  2018cites this paper
• Parasite biology: The reservoir hosts

  2018cites this paper
• Canine visceral leishmaniasis in Londrina, Paraná - investigation and case report

  2018cites this paper
• Prevalence of antibodies against visceralizing Leishmania spp. in brown rats from Grenada, West Indies

  2018cites this paper
• Richness and abundance of sand fly species in human settlements and degraded forest fragments in Maranhense Amazon

  year unknowncites this paper