Impacts of Long-term Fertilization on the Molecular Structure of Humic Acid and Organic Carbon Content in Soil Aggregates in Black Soil

Soil aggregates are the basic units of soil structure, and their composition and carbon (C) stability directly affect soil fertility. As cementing agents, humic substances play an important role in the formation and stability of soil aggregates. Long-term fertilization not only changes the structure of humic acid (HA), but also affects the content and stability of organic C in soil aggregates. In this study, based on a long-term fertilization experiment, the relationship between the molecular structure of HA and the stability of organic C in the aggregates was examined. Compared with the non-fertilization control (CK), both the application of organic manure alone (M) and organic manure combined with inorganic fertilizer application (MNPK) increased organic C content in the bulk soil and in HA. In addition, the application of organic manure (M, MNPK) favored the formation of macroaggregates (>0.25 mm) and showed a higher organic C contents of soil aggregates with different sizes than the CK. Moreover, the content of aliphatic C, the ratios of aliphatic C/aromatic C and alkyl C/O-alkyl C was increased with the application of organic fertilizer. A significant negative correlation was observed between aromatic C and organic C content of the aggregates with sizes of >2 mm, 2–0.25 mm, and 0.25–0.053 mm (P < 0.05). The findings indicated that organic fertilization treatments (M and MNPK) increased the aliphatic C content of HA, which favored the increase in the organic C content and stability of the aggregates.

• Publication year

  2019

• Venue

  Scientific Reports

• Publication date

  2019-08-15

• Fields of study

  Agricultural and Food Sciences, Medicine, Chemistry, Environmental Science

• Identifiers
  DOI 10.1038/s41598-019-48406-8PMID 31417124PMCID 6695396
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Effect of Long-Term Fertilization on Aggregate Size Distribution and Nutrient Accumulation in Aeolian Sandy Soil

  2022cited by this paper
• Evolution over years of structural characteristics of humic acids in Black Soil as a function of various fertilization treatments

  2018cited by this paper
• Effects of long-term fertilization on soil humic acid composition and structure in Black Soil

  2017cited by this paper
• Insights into the composition of recalcitrant organic matter from estuarine sediments using NMR spectroscopy

  2016cited by this paper
• The contentious nature of soil organic matter

  2015cited by this paper
• Persistence of soil organic matter as an ecosystem property

  2011cited by this paper
• Effects of Long-term Fertilization on Aggregate Size Distribution and Nutrient Content

  2011cited by this paper
• Effect of long-term application of organic fertilizer on structural characteristics of humin in black soil - a solid-state 13C NMR study.

  2009cited by this paper
• Effect of organic matter applications on 13C‐NMR spectra of humic acids of soil

  2008cited by this paper
• Particulate organic matter, carbohydrate, humic acid contents in soil macro- and microaggregates as affected by cultivation

  2006cited by this paper
• Changes of humic substances characteristics from forested to cultivated soils in Ethiopia

  2006cited by this paper
• Effect of base hydrolysis on the chemical composition of organic matter of an acid forest soil

  2005cited by this paper
• Effects of potassium humate on aggregate stability of two soils from Victoria, Australia

  2005cited by this paper
• Cycling and composition of organic matter in terrestrial and marine ecosystems

  2004cited by this paper
• A history of research on the link between (micro)aggregates, soil biota, and soil organic matter dynamics

  2004cited by this paper
• Characterization of organic matter in a northern hardwood forest soil by 13C NMR spectroscopy and chemical methods

  2003cited by this paper
• A REVIEW OF SOME IMPORTANT FAMILIES OF REFRACTORY MACROMOLECULES: COMPOSITION, ORIGIN, AND FATE IN SOILS AND SEDIMENTS

  2001cited by this paper
• Dynamics of soil organic matter associated with particle‐size fractions of water‐stable aggregates

  2000cited by this paper
• Root‐Derived Carbon and the Formation and Stabilization of Aggregates

  2000cited by this paper
• Role of hydrophobic components of soil organic matter in soil aggregate stability

  1999cited by this paper
• Radiocarbon Dating of Aliphatic Hydrocarbons A New Approach for Dating Passive-Fraction Carbon in Soil Horizons

  1999cited by this paper
• Fate of particulate organic matter in soil aggregates during cultivation

  1996cited by this paper
• Total and young organic matter distributions in aggregates of silty cultivated soils

  1995cited by this paper
• Carbon and Nitrogen Distribution in Aggregates from Cultivated and Native Grassland Soils

  1993cited by this paper
• Relationship between Soil Aliphatic Fraction Extracted with Supercritical Hexane, Soil Microbial Biomass, and Soil Aggregate Stability

  1990cited by this paper
• WATER‐REPELLENT SUBSTANCES AND AGGREGATE STABILITY IN HYDROPHOBIC SOIL

  1983cited by this paper
• Organic matter and water‐stable aggregates in soils

  1982cited by this paper

• Sustainable fertilizers from wastes: a strategy to enhance soil carbon, improve soil quality, and mitigate emissions

  2026cites this paper
• Recovery, Identification, and Presumptive Agricultural Application of Soil Bacteria

  2026cites this paper
• Influence of grazing patterns on the stability of soil aggregates in semi-arid grasslands

  2026cites this paper
• Autotrophic and Mixotrophic Microbial Carbon Assimilation During Organic Residue Decomposition in Mollisols: Mechanisms and Controls

  2026cites this paper
• Effects of Water–Fertilizer Management on Soil Aggregate Stability and Organic Carbon Sequestration in Greenhouse Eggplant Fields of the Black Soil Region

  2025cites this paper
• Study on the radon exhalation behavior of organic soils under high-temperature conditions

  2025cites this paper
• Exceptional roles by protozoa on enhancing degraded soil quality

  2025cites this paper
• Biochemical and molecular fingerprinting of soil organic matter fractions reveals diverse sources and stabilization mechanisms in Maritime Antarctica

  2025cites this paper
• Characteristics of ecosystem multifunctionality and influencing factors of different grassland types in temperate desert of Longzhong Loess Plateau

  2025cites this paper
• Distribution of humic substances and organic matter fraction: effects of long-term application of vermicompost

  2025cites this paper
• Soil–Plant Biochemical Interactions Under Agricultural Byproduct Amendments and Potassium Humate: Enhancing Soil Function and Bioactive Compounds in Sunflower Sprouts

  2025cites this paper
• Synergistic Effects of Organic and Chemical Fertilizers on Microbial-Mediated Carbon Stabilization: Insights from Metagenomics and Spectroscopy

  2025cites this paper
• The Improved Remediation Effect of the Combined Use of Earthworms with Bacillus subtilis-Loaded Biochar in Ameliorating Soda Saline–Alkali Soil

  2025cites this paper
• From waste to soil: The pivotal role of organic matter in soil aggregate formation under dry and wet conditions

  2025cites this paper
• Reduced soil water repellency suggests the need for timely replenishment of soil organic matter in long-term traditional farming

  2025cites this paper
• The Aggregate Structure and Organic Carbon Mineralization in Forest Soils Along an Elevation Gradient in the Sygera Mountains of the Southeastern Tibetan Plateau

  2025cites this paper
• How do moss-dominated biocrusts drive carbon sequestration under lithological constraints in karst soils?

  2025influential citation
• Effect of Organic and Bio-Fertilizers on Total Phosphorus, Organic Carbon, and the C/P Ratio in Rhizosphere and Non-Rhizosphere Soils

  2025cites this paper
• Enhancing soil health and tobacco productivity with different organic amendments: evidence from a 7-year field experiment

  2025cites this paper
• Enhancement of soil humic acid hydrophobicity by 5 consecutive years of full-amount straw shallow-mixed field return

  2024cites this paper
• Appropriate fertilization increases carbon and nitrogen sequestration and economic benefit for straw-incorporated upland farming

  2024cites this paper
• Effect of Organic Amendment and Mineral Fertilizer on Soil Aggregate Stability and Maize Yield on the Loess Plateau of China

  2024cites this paper
• Effect of cogongrass biochar enriched with nitrogen fertilizer dissolved in seaweed liquid extract on soil water content of Ultisol

  2024cites this paper
• Straw Addition Enhances Crop Yield, Soil Aggregation, and Soil Microorganisms in a 14-Year Wheat–Rice Rotation System in Central China

  2024cites this paper
• Effect of Aminating Lignin Loading with Arbuscular Mycorrhizal Fungi on Soil Aggregate Structure Improvement

  2024cites this paper
• Effects of Land Use Change on Soil Aggregate Stability and Erodibility in the Karst Region of Southwest China

  2024cites this paper
• Land use shapes the microbial community structure by altering soil aggregates and dissolved organic matter components

  2024cites this paper
• Evaluation of the soil aggregate stability under long term manure and chemical fertilizer applications: Insights from organic carbon and humic acid structure in aggregates

  2024cites this paper
• Assessing the formation and stability of paddy soil aggregate driven by organic carbon and Fe/Al oxides in rice straw cyclic utilization strategies: Insight from a six-year field trial.

  2024cites this paper
• Soil Mineral Nitrogen and Mobile Organic Carbon as Affected by Winter Wheat Strip Tillage and Forage Legume Intercropping

  2024cites this paper
• Evaluating Belizean Sugarcane Farmers’ Adoption Intentions Regarding Organic Fertilizer

  2024cites this paper
• Comparative analysis of humic substances of soil organic matter and liquid products from rice husks pyrolysis

  2024cites this paper
• Changes of soil organic carbon and aggregate stability along elevation gradient in Cunninghamia lanceolata plantations

  2024cites this paper
• Coupling amendment of microbial and compound fertilizers increases fungal necromass carbon and soil organic carbon by regulating microbial activity in flue-cured tobacco-planted field

  2023cites this paper
• Preparation and Molecular Structural Characterization of Fulvic Acid Extracted from Different Types of Peat

  2023cites this paper
• Formation of mineral‐associated organic matter in temperate soils is primarily controlled by mineral type and modified by land use and management intensity

  2023cites this paper
• Nano‐biochar: Properties and prospects for sustainable agriculture

  2023cites this paper
• The active compound in Rheum officinale Baill, aloe-emodin promotes tomato seedling growth

  2023cites this paper
• Effect of Mixture of Water Hyacinth Compost and Rice Husk Biochar on the Improvement of Alluvial Soil Properties and the Growth of Red Ginger (Zingiber Officinale L.)

  2023cites this paper
• Anaerobic digestion of primary winery wastewater sludge and evaluation of the character of the digestate as a potential fertilizer

  2022cites this paper
• Dynamics of increasing the yield of vetch-triticale grass mixture due to the use of a new complex biofertilizer

  2022cites this paper
• Impact of long-term nutrient management on sequestration and dynamics of soil organic carbon in a semi-arid tropical Alfisol of India

  2022cites this paper
• Conversion of pure Chinese fir plantation to multi-layered mixed plantation enhances the soil aggregate stability by regulating microbial communities in subtropical China

  2022cites this paper
• Long-Term Effects of Straw and Straw-Derived Biochar on Humic Substances and Aggregate-Associated Humic Substances in Brown Earth Soil

  2022cites this paper
• Stability of humic acid carbon under conservation agriculture practices

  2022cites this paper
• Application of organic and chemical fertilizers promoted the accumulation of soil organic carbon in farmland on the Loess Plateau

  2022cites this paper
• Understanding the Priming Effect and the Routes and Stocks of C in Incubated Soil with Residue Inputs

  2022cites this paper
• Effects of soil organic matter components and iron aluminum oxides on aggregate stability during vegetation succession in granite red soil eroded areas

  2022cites this paper
• The impact of long-term field experiments under different cropping systems on the molecular dynamics and stability of the soil Humeome

  2022cites this paper
• Tillage effects on humus composition and humic acid structural characteristics in soil aggregate-size fractions

  2021cites this paper
• Improving the fertilizer value of sugarcane wastes through phosphate rock amendment and phosphate-solubilizing bacteria inoculation

  2021cites this paper
• Organic carbon, total nitrogen, and microbial community distributions within aggregates of calcareous soil treated with biochar

  2021cites this paper
• Study on soil physical structure after the bioremediation of Pb pollution using microbial-induced carbonate precipitation methodology.

  2021cites this paper
• Response of the chemical structure of soil organic carbon to modes of maize straw return

  2021cites this paper
• Sustainable advances on phosphorus utilization in soil via addition of biochar and humic substances.

  2021cites this paper
• Molecular characterization of soil organic matter and its extractable humic fraction from long-term field experiments under different cropping systems

  2021cites this paper
• Chemical composition of soil organic carbon and aggregate stability along an elevation gradient in Helan Mountains, northwest China

  2021cites this paper
• The influence of organic and inorganic nutrient inputs on soil organic carbon functional groups content and maize yields

  2021cites this paper
• Changes in soil humus composition and humic acid structural characteristics under different corn straw returning modes

  2020cites this paper
• Optimization of reduced chemical fertilizer use in tea gardens based on the assessment of related environmental and economic benefits.

  2019cites this paper
• E ff ect of Aminating Lignin Loading with Arbuscular Mycorrhizal Fungi on Soil Aggregate Structure Improvement

  year unknowncites this paper
• THE EFFECTS OF THE APPLICATION OF ORGANIC AND MINERAL FERTILIZERS IN LONG-TERM EXPERIMENTS

  year unknowninfluential citation