MicroRNA-205 Regulates the Calcification and Osteoblastic Differentiation of Vascular Smooth Muscle Cells

Objectives: We investigated the role of miR-205 in the osteogenic differentiation of vascular smooth muscle cells (VSMCs). Methods: Osteogenic differentiation of human aortic smooth muscle cells (HASMCs) was induced by 10 mM β-glycerophosphate (β-GP). Alizarin Red S staining, alkaline phosphatase (ALP) activity and osteocalcin secretion were used to determine osteogenic differentiation of HASMCs. Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) was used to measure the expression of miR-205 in HASMCs. Results: The expression of endogenous miR-205 was decreased in HASMCs during β-glycerophosphate-induced calcification. Overexpression of miR-205 inhibited the differentiation of HASMCs into osteoblast-like cells, as evidenced by a decrease in ALP activity, osteocalcin secretion, and Runx2 expression, whereas miR-205 depletion enhanced osteoblastic differentiation of HASMCs. Runx2 and Smad1 were identified as direct targets of miR-205 by computational analysis and experimental assays. Conclusion: The present study shows that miR-205 may negatively regulate the β-glycerophosphate-induced calcification of HASMCs, at least partially by targeting Runx2 and Smad1.

• Publication year

  2014

• Venue

  Cellular Physiology and Biochemistry

• Publication date

  2014-06-27

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1159/000362971PMID 25012146
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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