Identification of the folate-binding proteins of rat liver mitochondria as dimethylglycine dehydrogenase and sarcosine dehydrogenase. Flavoprotein nature and enzymatic properties of the purified proteins.

The protein-bound folate of rat liver mitochondria (Zamierowski, M. M., and Wagner, C (1977) J. Biol. Chem. 252,933-938) is due to the presence of two folatebinding proteins, one of which has recently been identified as a M, = 90,000 flavoprotein with dimethylglycine dehydrogenase (EC 1.5.99.2) activity (Wittwer, A. J., and Wagner, C. (1980) Proc. Natl. Acad Sei. U. S. A. 77, 4484-4488). The other folate-binding protein appears to be a closely related enzyme, sarcosine dehydrogenase (EC 1.5.99.1) with a molecular weight of 105,000. Both proteins have been purified by gel filtration, DEAE-cellulose, and affinity chromatography. When mitochondrial folates were labeled by prior injection of [3H]folic acid, labeled tetrahydropteroylglutamate (tetrahydrofolic acid, H4PteGlu) and tetrahydropteroylpentaglutamate (H4PteGlus) were identified as the protein-bound folate derivatives. Both folatebinding proteins bound HJ‘teGlu, in vitro, and the protein with dimethylglycine dehydrogenase activity displayed greatest affinity for &PteGlus, followed by H4PteGlu, 5-formyl-H4PteGlu, and 5-methyLH4PteGlu. As a group, the reduced folates were bound 100-fold tighter than folic acid or methotrexate. Dissociation constants for H4PteGlu5 and H4PteGlu were estimated to be 0.2 and 0.4 PM, respectively. A stoichiometry of 1 mol of folate/mol of protein was indicated. The specificity and affinity of these binding proteins for H4PteGlu and H4PteGlus are consistent with their proposed enzymatic functions.

• Publication year

  1981

• Venue

  Journal of Biological Chemistry

• Publication date

  1981-04-25

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1016/s0021-9258(19)69572-4PMID 6163778
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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