Guanine nucleotide exchange factors operate by a simple allosteric competitive mechanism.

No abstract is available for this paper.

• Publication year

  2005

• Venue

  Biochemistry

• Publication date

  2005-11-29

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1021/BI0518601PMID 16300389
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• GEF means go: turning on RHO GTPases with guanine nucleotide-exchange factors

  2005cited by this paper
• Novel intermediate of Rac GTPase activation by guanine nucleotide exchange factor.

  2005cited by this paper
• The GAPs, GEFs, and GDIs of heterotrimeric G-protein alpha subunits

  2005cited by this paper
• Mechanism of the guanine nucleotide exchange reaction of Ras GTPase--evidence for a GTP/GDP displacement model.

  2005cited by this paper
• Always look on the bright site of Rho: structural implications for a conserved intermolecular interface

  2004cited by this paper
• Guanine nucleotide-exchange factors for arf GTPases: their diverse functions in membrane traffic.

  2004cited by this paper
• Structural evidence for feedback activation by Ras.GTP of the Ras-specific nucleotide exchange factor SOS.

  2003cited by this paper
• A growing family of guanine nucleotide exchange factors is responsible for activation of Ras-family GTPases.

  2002cited by this paper
• Exchange factors, effectors, GAPs and motor proteins: common thermodynamic and kinetic principles for different functions

  2002cited by this paper
• The Guanine Nucleotide-Binding Switch in Three Dimensions

  2001cited by this paper
• Vps9, Rabex-5 and DSS4: proteins with weak but distinct nucleotide-exchange activities for Rab proteins.

  2001cited by this paper
• Mechanism of nucleotide release from Rho by the GDP dissociation stimulator protein.

  2000cited by this paper
• Regulation of GTPases in the Bacterial Translation Machinery

  2000cited by this paper
• GEFs: structural basis for their activation of small GTP-binding proteins.

  1999cited by this paper
• Kinetic analysis by fluorescence of the interaction between Ras and the catalytic domain of the guanine nucleotide exchange factor Cdc25Mm.

  1998cited by this paper
• The kinetic mechanism of Ran--nucleotide exchange catalyzed by RCC1.

  1995cited by this paper
• Guanine nucleotide exchange factors: Activators of Ras superfamily proteins

  1995cited by this paper
• Kinetic and structural analysis of the Mg(2+)-binding site of the guanine nucleotide-binding protein p21H-ras.

  1993cited by this paper
• Is there a rate-limiting step before GTP cleavage by H-ras p21?

  1991cited by this paper
• Kinetics of interaction of nucleotides with nucleotide-free H-ras p21.

  1990cited by this paper
• The ternary complex formed between actin, myosin subfragment 1 and ATP (beta, gamma-NH).

  1978cited by this paper

• Genomic signatures of rapid local selection and introgression drive adaptive phylogeography in the European river skater

  2026cites this paper
• The biochemical mechanism of Rho GTPase membrane binding, activation and retention in activity patterning

  2025cites this paper
• Allosteric nanobodies to study the interactions between SOS1 and RAS

  2024cites this paper
• Inhibition of CD82 improves colitis by increasing NLRP3 deubiquitination by BRCC3

  2023cites this paper
• Competitive, connective and allosteric Nanobodies that modulate the SOS1•RAS protein-protein interactions and tune the nucleotide exchange rate as a starting point for drug design

  2023cites this paper
• PE_PGRS38 Interaction With HAUSP Downregulates Antimycobacterial Host Defense via TRAF6

  2022cites this paper
• ChILL & DisCO to discover competitive, connective and allosteric Nanobodies that modulate the SOS1•RAS protein-protein interactions and tune the nucleotide exchange

  2022cites this paper
• A Comparative Analysis of RAS Variants in Patients with Disorders of Somatic Mosaicism.

  2022cites this paper
• The RHO Family GTPases: Mechanisms of Regulation and Signaling

  2021cites this paper
• Methods to Monitor Ras Activation State.

  2021cites this paper
• Multi-Functional MPT Protein as a Therapeutic Agent against Mycobacterium tuberculosis

  2021cites this paper
• Assembly and Regulation of CRL Ubiquitin Ligases.

  2020cites this paper
• The Ras switch in structural and historical perspective

  2019cites this paper
• Mutant-Specific Targeting of Ras G12C Activity by Covalently Reacting Small Molecules.

  2019cites this paper
• Cand1-Mediated Adaptive Exchange Mechanism Enables Variation in F-Box Protein Expression.

  2018cites this paper
• Activation of Rho Family GTPases by Small Molecules

  2018cites this paper
• Structural fingerprints, interactions, and signaling networks of RAS family proteins beyond RAS isoforms

  2018cites this paper
• A Single-molecule Approach to Study Multimeric Molecular Motors and Optimal Thermodynamic Length

  2017cites this paper
• Nucleotide based covalent inhibitors of KRas can only be efficient in vivo if they bind reversibly with GTP-like affinity

  2017cites this paper
• SCIMP is a transmembrane non-TIR TLR adaptor that promotes proinflammatory cytokine production from macrophages

  2017cites this paper
• C9orf72: At the intersection of lysosome cell biology and neurodegenerative disease

  2017cites this paper
• Mathematical analysis of a spatially coupled reaction-diffusion system for signaling networks in biological cells

  2016cites this paper
• A pull-down procedure for the identification of unknown GEFs for small GTPases

  2016cites this paper
• Review: Ras GTPases and myosin: Qualitative conservation and quantitative diversification in signal and energy transduction

  2016cites this paper
• An attenuated phenotype of Costello syndrome in three unrelated individuals with a HRAS c.179G>A (p.Gly60Asp) mutation correlates with uncommon functional consequences

  2015cites this paper
• Structural outline of the detailed mechanism for elongation factor Ts-mediated guanine nucleotide exchange on elongation factor Tu.

  2015cites this paper
• How not to do kinetics: examples involving GTPases and guanine nucleotide exchange factors

  2014cites this paper
• A homogeneous quenching resonance energy transfer assay for the kinetic analysis of the GTPase nucleotide exchange reaction

  2014cites this paper
• Classical Rho Proteins: Biochemistry of Molecular Switch Function and Regulation

  2014cites this paper
• Segregation and Crosstalk of D1 Receptor-Mediated Activation of ERK in Striatal Medium Spiny Neurons upon Acute Administration of Psychostimulants

  2014cites this paper
• Functional analysis of a duplication (p.E63_D69dup) in the switch II region of HRAS: new aspects of the molecular pathogenesis underlying Costello syndrome.

  2013cites this paper
• Selectivity of CDC25 homology domain-containing guanine nucleotide exchange factors.

  2013cites this paper
• Diverging gain-of-function mechanisms of two novel KRAS mutations associated with Noonan and cardio-facio-cutaneous syndromes.

  2013cites this paper
• Cand1 promotes assembly of new SCF complexes through dynamic exchange of F box proteins.

  2013influential citation
• Quantitative Analysis of Guanine Nucleotide Exchange Factors (GEFs) as Enzymes

  2013cites this paper
• Quantitative a nalysis of Guanine Nucleotide exchange Factors (G eFs) as e nzymes

  2013cites this paper
• Kinetics of Interaction between ADP-ribosylation Factor-1 (Arf1) and the Sec7 Domain of Arno Guanine Nucleotide Exchange Factor, Modulation by Allosteric Factors, and the Uncompetitive Inhibitor Brefeldin A

  2012cites this paper
• The Interaction Properties of the Human Rab GTPase Family – A Comparative Analysis Reveals Determinants of Molecular Binding Selectivity

  2012cites this paper
• Nucleotide exchange factors

  2012cites this paper
• Catalytic Mechanisms in Sec7 and Vps9 Domain Exchange Factors for Arf and Rab GTPases: A Dissertation

  2012influential citation
• Germline KRAS mutations cause aberrant biochemical and physical properties leading to developmental disorders

  2011cites this paper
• Turing instabilities in a mathematical model for signaling networks

  2011cites this paper
• Duplication of Glu37 in the switch I region of HRAS impairs effector/GAP binding and underlies Costello syndrome by promoting enhanced growth factor-dependent MAPK and AKT activation.

  2010cites this paper
• The spatial control of Rap1 and Epac proteins

  2010cites this paper
• Kinetic analysis of the guanine nucleotide exchange activity of TRAPP, a multimeric Ypt1p exchange factor.

  2009cites this paper
• Characterisation of the nucleotide exchange factor ITSN1L: evidence for a kinetic discrimination of GEF-stimulated nucleotide release from Cdc42.

  2009cites this paper
• Oncogenic HRAS mutations cause prolonged PI3K signaling in response to epidermal growth factor in fibroblasts of patients with Costello syndrome

  2009cites this paper
• Studies of Proteins that Regulate Melanin Synthesis and Distribution

  2009cites this paper
• Structural Mechanisms for Regulation of Membrane Traffic by Rab GTPases

  2009cites this paper
• Differences in flexibility underlie functional differences in the Ras activators son of sevenless and Ras guanine nucleotide releasing factor 1.

  2009cites this paper
• In vitro GEF and GAP assays.

  2009cites this paper
• Architecture-dependent noise discriminates functionally analogous differentiation circuits.

  2009cites this paper
• Coordinate Regulation of G Protein Signaling via Dynamic Interactions of Receptor and GAP

  2008cites this paper
• Membrane-dependent signal integration by the Ras activator Son of sevenless

  2008cites this paper
• The role of the conserved switch II glutamate in guanine nucleotide exchange factor-mediated nucleotide exchange of GTP-binding proteins.

  2008cites this paper
• Erratum: Membrane-dependent signal integration by the Ras activator Son of sevenless

  2008cites this paper
• Ras Is Required for the Cyclic AMP-Dependent Activation of Rap1 via Epac2

  2008cites this paper
• Structural basis for Rab GTPase activation by VPS9 domain exchange factors

  2007cites this paper
• Strukturelle und biochemische Einblicke in Mechanismen des Nukleotidaustauschs von Rab-Proteinen

  2007cites this paper
• Sec2 is a highly efficient exchange factor for the Rab protein Sec4.

  2007cites this paper
• Asef is a Cdc42-specific guanine nucleotide exchange factor

  2007cites this paper
• Regulatorische Mechanismen in der

  2006cites this paper
• Mg2+ and a Key Lysine Modulate Exchange Activity of Eukaryotic Translation Elongation Factor 1Bα*

  2006cites this paper
• A Ras-induced conformational switch in the Ras activator Son of sevenless

  2006cites this paper