PMK-1 p38 MAPK promotes cadmium stress resistance, the expression of SKN-1/Nrf and DAF-16 target genes, and protein biosynthesis in Caenorhabditis elegans

The mechanisms of cadmium (Cd) resistance are complex and not sufficiently understood. The present study, therefore, aimed at assessing the roles of important components of stress-signaling pathways and of ABC transporters under severe Cd stress in Caenorhabditis elegans. Survival assays on mutant and control animals revealed a significant promotion of Cd resistance by the PMK-1 p38 MAP kinase, the transcription factor DAF-16/FoxO, and the ABC transporter MRP-1. Transcriptome profiling by RNA-Seq on wild type and a pmk-1 mutant under control and Cd stress conditions revealed, inter alia, a PMK-1-dependent promotion of gene expression for the translational machinery. PMK-1 also promoted the expression of target genes of the transcription factors SKN-1/Nrf and DAF-16 in Cd-stressed animals, which included genes for molecular chaperones or immune proteins. Gene expression studies by qRT-PCR confirmed the positive effects of PMK-1 on DAF-16 activity under Cd stress and revealed negative effects of DAF-16 on the expression of genes for MRP-1 and DAF-15/raptor. Additional studies on pmk-1 RNAi-treated wild type and mutant strains provided further information on the effects of PMK-1 on SKN-1 and DAF-16, which resulted in a model of these relationships. The results of this study demonstrate a central role of PMK-1 for the processing of cellular responses to abiotic and biotic stressors, with the promoting effects of PMK-1 on Cd resistance mostly mediated by the transcription factors SKN-1 and DAF-16.

• Publication year

  2017

• Venue

  Zeitschrift für Induktive Abstammungs- und Vererbungslehre

• Publication date

  2017-08-01

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1007/s00438-017-1351-zPMID 28766017PMCID 5682872
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Detoxification and sensing mechanisms are of similar importance for Cd resistance in Caenorhabditis elegans

  2016cited by this paper
• SKN-1/Nrf, stress responses, and aging in Caenorhabditis elegans.

  2015cited by this paper
• ttm-1 Encodes CDF Transporters That Excrete Zinc from Intestinal Cells of C. elegans and Act in a Parallel Negative Feedback Circuit That Promotes Homeostasis

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• The p38 MAPK PMK-1 shows heat-induced nuclear translocation, supports chaperone expression, and affects the heat tolerance of Caenorhabditis elegans

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• Role of MTL-1, MTL-2, and CDR-1 in mediating cadmium sensitivity in Caenorhabditis elegans.

  2012influential reference
• TOR signaling and rapamycin influence longevity by regulating SKN-1/Nrf and DAF-16/FoxO.

  2012cited by this paper
• Revelations from the Nematode Caenorhabditis elegans on the Complex Interplay of Metal Toxicological Mechanisms

  2011cited by this paper
• Sch9 regulates ribosome biogenesis via Stb3, Dot6 and Tod6 and the histone deacetylase complex RPD3L

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  2010cited by this paper
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  2010cited by this paper
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  2010cited by this paper
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  2010cited by this paper
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  2010cited by this paper
• Control the diameters of mesoporous silica nanotubes by varying stirring time.

  2010cited by this paper
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  2009cited by this paper
• Condition‐adapted stress and longevity gene regulation by Caenorhabditis elegans SKN‐1/Nrf

  2009influential reference
• A cadmium toxicity assay using stress responsive Caenorhabditis elegans mutant strains.

  2009cited by this paper
• C. elegans are protected from lethal hypoxia by an embryonic diapause.

  2009cited by this paper
• A Role for p38 Stress-Activated Protein Kinase in Regulation of Cell Growth via TORC1

  2009cited by this paper
• Direct inhibition of the longevity-promoting factor SKN-1 by insulin-like signaling in C. elegans.

  2008cited by this paper
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  2008cited by this paper
• Cadmium-induced germline apoptosis in Caenorhabditis elegans: the roles of HUS1, p53, and MAPK signaling pathways.

  2008cited by this paper
• The MAP kinase JNK‐1 of Caenorhabditis elegans: Location, activation, and influences over temperature‐dependent insulin‐like signaling, stress responses, and fitness

  2008cited by this paper
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  2008cited by this paper
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  2008cited by this paper
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• Single and double metallothionein knockout in the nematode C. elegans reveals cadmium dependent and independent toxic effects on life history traits.

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  2007cited by this paper
• Toxicogenomic analysis of Caenorhabditis elegans reveals novel genes and pathways involved in the resistance to cadmium toxicity

  2007cited by this paper
• p38 MAPK Regulates Expression of Immune Response Genes and Contributes to Longevity in C. elegans

  2006cited by this paper
• The search for DAF-16/FOXO transcriptional targets: approaches and discoveries.

  2006cited by this paper
• Nuclear hormone receptors in C. elegans.

  2006cited by this paper
• Human multidrug resistance ABCB and ABCG transporters: participation in a chemoimmunity defense system.

  2006cited by this paper
• Cadmium Toxicity Monitoring Using Stress Related Gene Expressions in Caenorhabditis elegans

  2006cited by this paper
• TOR signaling in growth and metabolism.

  2006cited by this paper
• Maintenance of C. elegans.

  2006cited by this paper
• The p38 signal transduction pathway participates in the oxidative stress-mediated translocation of DAF-16 to Caenorhabditis elegans nuclei.

  2005cited by this paper
• The C. elegans p38 MAPK pathway regulates nuclear localization of the transcription factor SKN-1 in oxidative stress response.

  2005cited by this paper
• Analysis of long-lived C. elegans daf-2 mutants using serial analysis of gene expression.

  2005cited by this paper
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  2005cited by this paper
• Regulation of the Caenorhabditis elegans oxidative stress defense protein SKN-1 by glycogen synthase kinase-3.

  2005cited by this paper
• Multidrug resistance-associated protein MRP-1 regulates dauer diapause by its export activity in Caenorhabditis elegans

  2005cited by this paper
• Mitogen-activated protein kinase pathways defend against bacterial pore-forming toxins.

  2004cited by this paper
• C. elegans metallothioneins: new insights into the phenotypic effects of cadmium toxicosis.

  2004cited by this paper
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  2004cited by this paper
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  2004cited by this paper
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  2004cited by this paper
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  2004cited by this paper
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  2003cited by this paper
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  2003influential reference
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  2003cited by this paper
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  2002cited by this paper
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  2001cited by this paper
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  2001cited by this paper
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  2001cited by this paper
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  2000cited by this paper
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  2000cited by this paper
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  2000cited by this paper
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  1998cited by this paper
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  1998cited by this paper
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  1998cited by this paper
• The significance of digital gene expression profiles.

  1997cited by this paper
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  1997cited by this paper
• Genetic and Environmental Regulation of Dauer Larva Development

  1997cited by this paper
• Cadmium, gene regulation, and cellular signalling in mammalian cells.

  1997cited by this paper
• Brazilian Journal of Medical and Biological Research (1996) 29: 1715-1733 OXIDATIVE STRESS: ANIMAL ADAPTATIONS IN NATURE

  1996cited by this paper
• THE FUNCTIONS AND REGULATION OF GLUTATHIONE S-TRANSFERASES IN PLANTS.

  1996cited by this paper
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  1996influential reference
• The novel metallothionein genes of Caenorhabditis elegans. Structural organization and inducible, cell-specific expression.

  1993cited by this paper

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