N‐terminal processing of affinity‐tagged recombinant proteins purified by IMAC procedures

No abstract is available for this paper.

• Publication year

  2015

• Venue

  Journal of Molecular Recognition

• Publication date

  2015-07-01

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1002/jmr.2456PMID 25727088
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Studies with an immobilized metal affinity chromatography cassette system involving binuclear triazacyclononane-derived ligands: automation of batch adsorption measurements with tagged recombinant proteins.

  2014cited by this paper
• Metal complexes of new bis(tacn) ligands: Syntheses and structures of copper(II) complexes

  2014influential reference
• Synthesis and Radiolabelling of DOTA-Linked Glutamine Analogues with 67,68Ga as Markers for Increased Glutamine Metabolism in Tumour Cells

  2013cited by this paper
• His-rich sequences – is plagiarism from nature a good idea?

  2013cited by this paper
• Application of an IMAC cassette for the purification of N-terminally tagged proteins

  2013influential reference
• Reliable, Large‐Scale Cleavage of Tags from Affinity‐Purified Biopharmaceuticals

  2013cited by this paper
• Real time analysis of protein location and function: A Golgi‐specific PKD sensor

  2012cited by this paper
• Protease-immobilized Microreactor for Rapid and Site-specific Affinity Tag Cleavage

  2012cited by this paper
• The two faces of His‐tag: Immune response versus ease of protein purification

  2012cited by this paper
• Increased adsorption of histidine-tagged proteins onto tissue culture polystyrene.

  2012cited by this paper
• An efficient synthesis of 1,3-bis(1,4,7-triazacyclonon-1-yl)-2-hydroxypropane, [1,3-bis(TACN)-2-propanol]

  2012cited by this paper
• Immobilized metal ion affinity chromatography: a review on its applications

  2012cited by this paper
• Histidine affinity tags affect MSP142 structural stability and immunodominance in mice

  2012cited by this paper
• Purification of poly-histidine-tagged proteins.

  2011cited by this paper
• Comparison of the binding behavior of several histidine-containing proteins with immobilized copper(II) complexes of 1,4,7-triazacyclononane and 1,4-bis(1,4,7-triazacyclononan-1-yl)butane.

  2011cited by this paper
• A simple method for controlled immobilization of proteins on modified SAMs.

  2011cited by this paper
• Use of phage display methods to identify heptapeptide sequences for use as affinity purification 'tags' with novel chelating ligands in immobilized metal ion affinity chromatography.

  2011influential reference
• Exploiting the interactions between poly-histidine fusion tags and immobilized metal ions

  2011cited by this paper
• Immobilized metal ion affinity chromatography.

  2011cited by this paper
• Effects of IMAC specific peptide tags on the stability of recombinant green fluorescent protein

  2011influential reference
• Immobilized-metal affinity chromatography adsorbent with paramagnetism and its application in purification of histidine-tagged proteins

  2010cited by this paper
• Immobilized-metal affinity chromatography (IMAC): a review.

  2009cited by this paper
• Chapter 27 Immobilized-Metal Affinity Chromatography (IMAC)

  2009cited by this paper
• Comparison of the efficiencies of different affinity tags in the purification of a recombinant secretory protein expressed in silkworm larval hemolymph

  2009cited by this paper
• Separation of hexahistidine fusion proteins with immobilized metal ion affinity chromatographic (IMAC) sorbents derived from MN+‐tacn and its derivatives

  2009cited by this paper
• Rapid Matrix‐Assisted Refolding of Histidine‐Tagged Proteins

  2009cited by this paper
• Oligohis‐tags: mechanisms of binding to Ni2+‐NTA surfaces

  2009cited by this paper
• Immobilized metal ion affinity systems for recovery and structure function studies of proteins at molecular, supramolecular and the cellular levels

  2008cited by this paper
• Enhancing the specificity of the enterokinase cleavage reaction to promote efficient cleavage of a fusion tag.

  2008cited by this paper
• Immobilized Metal‐Ion Affinity Chromatography: Status and Trends

  2007cited by this paper
• Dipeptidyl peptidase II (DPPII), a review.

  2007cited by this paper
• Double-hexahistidine tag with high-affinity binding for protein immobilization, purification, and detection on ni-nitrilotriacetic acid surfaces.

  2006cited by this paper
• Current strategies for the use of affinity tags and tag removal for the purification of recombinant proteins.

  2006cited by this paper
• Neutral additives enhance the metal-chelate affinity adsorption of nucleic acids: role of water activity.

  2006cited by this paper
• Solubility-enhancing proteins MBP and NusA play a passive role in the folding of their fusion partners.

  2006cited by this paper
• Influence of the protein oligomericity on final yield after affinity tag removal in purification of recombinant proteins.

  2006cited by this paper
• Efficient production of a soluble fusion protein containing human beta-defensin-2 in E. coli cell-free system.

  2005cited by this paper
• Attachment of a histidine tag to the minimal zinc finger protein of the Aspergillus nidulans gene regulatory protein AreA causes a conformational change at the DNA-binding site.

  2005cited by this paper
• Imidazole derivatives of binuclear copper (II) and nickel (II) complexes incorporating bis(1,4,7-triazacyclononan-1-yl) ligands

  2005influential reference
• Potential for Using Histidine Tags in Purification of Proteins at Large Scale

  2005cited by this paper
• Peptidase Activity of the Escherichia coli Hsp31 Chaperone*

  2004cited by this paper
• The use of recombinant methods and molecular engineering in protein crystallization.

  2004cited by this paper
• Cloning, Expression, Purification and Bioassay of Human Vasostatin

  2003cited by this paper
• A critical review of the methods for cleavage of fusion proteins with thrombin and factor Xa.

  2003cited by this paper
• ON THE PROTEOLYTIC ENZYMES OF ANIMAL TISSUES

  2003cited by this paper
• Attachment of Histidine Tags to Recombinant Tumor Necrosis Factor-Alpha Drastically Changes Its Properties

  2002cited by this paper
• Applications of novel affinity cassette methods: use of peptide fusion handles for the purification of recombinant proteins

  2001cited by this paper
• A Simple Biochemical Assay for Glutathione S-Transferase Activity and Its Possible Field Application for Screening Glutathione S-Transferase-Based Insecticide Resistance

  2000influential reference
• Physicochemical factors in polypeptide and protein purification and analysis by high-performance liquid chromatographic techniques: current status and challenges for the future

  2000cited by this paper
• Designing new metal affinity peptides by random mutagenesis of a natural metal-binding site.

  2000cited by this paper
• Removal of N-terminal polyhistidine tags from recombinant proteins using engineered aminopeptidases.

  1999influential reference
• Characterisation by immobilised metal ion affinity chromatographic procedures of the binding behaviour of several synthetic peptides designed to have high affinity for Cu(II) ions.

  1999cited by this paper
• Instability, stabilization, and formulation of liquid protein pharmaceuticals.

  1999cited by this paper
• Interdependency of sequence and positional specificities for cysteine proteases of the papain family.

  1999cited by this paper
• Histidines in affinity tags and surface clusters for immobilized metal-ion affinity chromatography of trimeric tumor necrosis factor alpha.

  1999cited by this paper
• Expression of maize gamma zein C-terminus in Escherichia coli.

  1998cited by this paper
• Selection of optimum affinity tags from a phage-displayed peptide library. Application to immobilized copper(II) affinity chromatography.

  1997cited by this paper
• Coordination Modes of a Series of Xylylene-Bridged Bis(1,4,7-triazacyclonon-1-yl) Ligands: Synthesis, Structure, and Properties of Nickel(II) and Copper(II) Complexes

  1997cited by this paper
• Acute nephrotoxicity of a carcinogenic iron chelate Selective inhibition of a proteolytic conversion of α 2U‐globulin to the kidney fatty acid‐binding protein

  1995cited by this paper
• A silver stain protocol for proteins yielding high resolution and transparent background in sodium dodecyl sulfate‐polyacrylamide gels

  1995cited by this paper
• Multiple-site binding interactions in metal-affinity chromatography. I. Equilibrium binding of engineered histidine-containing cytochromes c.

  1994cited by this paper
• Purification of tryptophan containing synthetic peptides by selective binding of the alpha-amino group to immobilised metal ions.

  1994influential reference
• Immobilized metal affinity chromatography (IMAC).

  1994cited by this paper
• Separation and analysis of peptides and proteins.

  1993cited by this paper
• High-performance liquid chromatography of amino acids, peptides and proteins: CXXI. 8-Hydroxyquinoline-metal chelate chromatographic support: an additional mode of selectivity in immobilized-metal affinity chromatography

  1992cited by this paper
• Immobilized Metal Ion Affinity Chromatography (IMAC) Chemistry and Bioseparation Applications

  1991cited by this paper
• Metal-Affinity Separations: A New Dimension in Protein Processing

  1991cited by this paper
• Characterization of His-X3-His sites in alpha-helices of synthetic metal-binding bovine somatotropin.

  1991influential reference
• Characterization of His-X3-His sites in α-helices of synthetic metal-binding bovine somatotropin

  1991cited by this paper
• Surface topography of histidine residues: a facile probe by immobilized metal ion affinity chromatography.

  1989cited by this paper
• The saga of IMAC and MIT.

  1989influential reference
• IMAC—Immobilized metal ion affinity based chromatography

  1988cited by this paper
• Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase.

  1988cited by this paper
• Genetic Approach to Facilitate Purification of Recombinant Proteins with a Novel Metal Chelate Adsorbent

  1988cited by this paper
• Chemical characterization of recombinant human leukocyte interferon A using fast atom bombardment mass spectrometry.

  1987cited by this paper
• New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues.

  1987cited by this paper
• Expression of bovine growth hormone derivatives in Escherichia coli and the use of the derivatives to produce natural sequence growth hormone by cathepsin C cleavage.

  1987cited by this paper
• Purification of proteins by IMAC

  1985cited by this paper
• New observations on the substrate specificity of cathepsin C (dipeptidyl aminopeptidase I). Including the degradation of beta-corticotropin and other peptide hormones.

  1969cited by this paper
• On the proteolytic enzymes of animal tissues; an intracellular enzyme related to chymotrypsin.

  1948cited by this paper

• Fundamentals of protein affinity chromatography: How to prepare and properly use an affinity matrix.

  2025cites this paper
• Promiscuity of host cell proteins in the purification of histidine tagged recombinant xylanase A by IMAC procedures: A case study with a Ni2+-tacn-based IMAC system.

  2019cites this paper
• A Simple Method for High-Level Expression of HTLV-1 gp46-I(162-214) Peptide and Its Application for Screening

  2018cites this paper
• Production of human pro-relaxin H2 in the yeast Pichia pastoris

  2017cites this paper