Replication Fork Reversal Occurs Spontaneously after Digestion but Is Constrained in Supercoiled Domains*

Replication fork reversal was investigated in undigested and linearized replication intermediates of bacterial DNA plasmids containing a stalled fork. Two-dimensional agarose gel electrophoresis, a branch migration and extrusion assay, electron microscopy, and DNA-psoralen cross-linking were used to show that extensive replication fork reversal and extrusion of the nascent-nascent duplex occurs spontaneously after DNA nicking and restriction enzyme digestion but that fork retreat is severely limited in covalently closed supercoiled domains.

• Publication year

  2007

• Venue

  Journal of Biological Chemistry

• Publication date

  2007-06-22

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1074/jbc.M701559200PMID 17456472
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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