A 6-nm ultra-photostable DNA FluoroCube for fluorescence imaging

Photobleaching limits extended imaging of fluorescent biological samples. We developed DNA-based ‘FluoroCubes’ that are similar in size to the green fluorescent protein, have single-point attachment to proteins, have a ~54-fold higher photobleaching lifetime and emit ~43-fold more photons than single organic dyes. We demonstrate that DNA FluoroCubes provide outstanding tools for single-molecule imaging, allowing the tracking of single motor proteins for >800 steps with nanometer precision. DNA-based FluoroCubes are ~6 nm small, monovalent probes that contain six organic dyes, which confer dramatic photostability relative to single dyes and offer more uniform signal compared to quantum dots for extended biological imaging.

• Publication year

  2019

• Venue

  bioRxiv

• Publication date

  2019-07-27

• Fields of study

  Biology, Materials Science, Physics, Chemistry, Medicine

• Identifiers
  DOI 10.1038/s41592-020-0782-3PMID 32203385PMCID 7138518
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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