Nitric oxide regulates glutathione synthesis and cold tolerance in forage legumes

Abstract The role of nitric oxide (NO) in cold acclimation through regulation on glutathione synthesis was investigated in Medicago falcata, Medicago truncatula and NITRATE REDUCTASE 1 (NIA1) mutants nia1-1 and nia1-2 of M. truncatula. M. falcata is an important forage legume with great cold tolerance, while M. truncatua is a model legume with lower cold tolerance than M. falcata. Glutathione synthesis and redox were promoted in both species in response to cold treatment, which showed an association with cold induced γ-glutamylcysteine synthetase (γ-ECS) and glutathione reductase (GR) activities with increased transcript levels of γ-ECS1, glutathione synthetase1 (GSHS1), GSHS2, GR1 and GR2 in both species. The cold induced glutathione concentration, glutathione redox, activities of γ-ECS and GR, and transcripts of γ-ECS1, GSHS1, GSHS2, GR1 and GR2 were suppressed by pretreatment with either NR inhibitor or NO scavenger in M. falcata and M. truncatula or by knock-out of NIA1 in nia1-1 and nia1-2 mutants. Moreover, treatment with exogenous NO resulted in the similar alterations in glutathione synthesis and redox as well as levels in enzyme activity and transcripts as cold treatment. The results suggested that NR-derived NO production is involved in cold regulated glutathione synthesis and redox by regulating gene expression. Higher levels of glutathione concentration, glutathione redox, activities of γ-ECS and GR, and transcripts of γ-ECS1, GSHS1, GSHS2, GR1 and GR2 were observed in M. falcata than in M. truncatula after cold treatment, suggesting that the difference in glutathione synthesis and redox is associated with the differential cold tolerance between the two species.

• Publication year

  2019

• Venue

  Environmental and Experimental Botany

• Publication date

  2019-11-01

• Fields of study

  Biology, Agricultural and Food Sciences, Chemistry, Environmental Science

• Identifiers
  DOI 10.1016/J.ENVEXPBOT.2019.103851
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

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