De novo Analysis of Heortia vitessoides (Lepidoptera: Crambidae) Transcriptome and Identification of Putative Cytochrome P450 Monooxygenase Genes

Abstract We analyzed a transcriptome generated from four developmental stages of Heortia vitessoides Moore (Lepidoptera: Crambidae), a defoliator of the agarwood tree, Aquilaria sinensis (Lour.) Gilg (Thymelaeaceae). A total of 110.53 Mb clean reads were produced, and de novo assembled into 42,946 unigenes of an average length of 1,059 base pairs (bp). Among these unigenes, 22,106 (51.47%) showed significant similarity (E-value <10–5) to known proteins in the National Center for Biotechnology Information (NCBI) nonredundant database. Gene ontology (GO), eukaryotic ortholog groups (KOG), and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were used to classify the functionality of unigenes. Moreover, 46 putative cytochrome P450 (CYP) monooxygenase genes were identified from this dataset. These CYP genes were classified into four clans consisting of 22 families and 34 subfamilies. The expression profiles of genes belonging to CYP4 and CYP6 families and exposed to half the lethal concentrations (LC50) of chlorantraniliprole and beta-cypermethrin were determined by real-time reverse transcription-quantitative PCR (RT-qPCR). The results showed that the transcription levels of eight (CYP4M39, CYP6AB49, CYP6AB53, CYP6AB61, CYP6AE17, CYP6AW1, CYP6BD6, CYP6CV1) and five (CYP6AB10, CYP6AB53, CYP6AE12, CYP6AE17, CYP6BD6) genes significantly increased in the fourth-instar larvae following exposure to the insecticides chlorantraniliprole and beta-cypermethrin, respectively. Therefore, these genes are potential candidates involved in the detoxification of these two insecticides. Further studies utilizing the RNA interference approach are required to enhance our understanding of the functionality of these genes in this forest pest.

• Publication year

  2019

• Venue

  Journal of entomological science

• Publication date

  2019-08-06

• Fields of study

  Biology, Environmental Science

• Identifiers
  DOI 10.18474/JES18-103
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

• Identification of putative cytochrome P450 monooxygenase genes from the small white butterfly, Pieris rapae (Lepidoptera: Pieridae), and their response to insecticides.

  2018cited by this paper
• Geographic Variation in Larval Metabolic Rate Between Northern and Southern Populations of the Invasive Gypsy Moth

  2018cited by this paper
• Determination of the Baseline Susceptibility of European Populations of Cydia pomonella (Lepidoptera: Tortricidae) to Chlorantraniliprole and the Role of Cytochrome P450 Monooxygenases.

  2018influential reference
• Identification and Functional Analysis of a Novel Cytochrome P450 Gene CYP9A105 Associated with Pyrethroid Detoxification in Spodoptera exigua Hübner

  2018cited by this paper
• Multiple Glutathione S-Transferase Genes in Heortia vitessoides (Lepidoptera: Crambidae): Identification and Expression Patterns

  2018cited by this paper
• Soil Moisture Effects on Pupation Behavior, Physiology, and Morphology of Heortia vitessoides (Lepidoptera: Crambidae)

  2017cited by this paper
• Identification of a novel cytochrome P450 CYP321B1 gene from tobacco cutworm (Spodoptera litura) and RNA interference to evaluate its role in commonly used insecticides

  2017cited by this paper
• De novo assembly and analysis of the Heortia vitessoides transcriptome via high-throughput Illumina sequencing

  2017cited by this paper
• De Novo Assembly of the Transcriptome for Oriental Armyworm Mythimna separata (Lepidoptera: Noctuidae) and Analysis on Insecticide Resistance-Related Genes

  2016cited by this paper
• Insecticidal Activity and Insect Repellency of Four Species of Sea Lily (Comatulida: Comatulidae) From Taiwan

  2016cited by this paper
• Effect of Substrate Type and Moisture on Pupation and Emergence of Heortia vitessoides (Lepidoptera: Crambidae): Choice and No-Choice Studies

  2016cited by this paper
• CHARACTERIZATION AND EXPRESSION PROFILES OF FIVE POSSIBLE CYTOCHROME P450 GENES FROM Liposcelis entomophila (ENDERLEIN) (PSOCOPTERA: LIPOSCELIDIDAE).

  2016cited by this paper
• Aggregation and Feeding Preference of Gregarious Heortia vitessoides (Lepidoptera: Crambidae) Larvae to Aquilaria sinensis (Thymelaeaceae)

  2016cited by this paper
• Characterization and expression of the cytochrome P450 gene family in diamondback moth, Plutella xylostella (L.)

  2015cited by this paper
• Identification and characterisation of multiple glutathione S-transferase genes from the diamondback moth, Plutella xylostella.

  2015cited by this paper
• Identification and expression profiles of putative cytochrome P450 monooxygenase genes from Cnaphalocrocis medinalis (Lepidoptera: Pyralidae)

  2015cited by this paper
• Genomic and transcriptomic insights into the cytochrome P450 monooxygenase gene repertoire in the rice pest brown planthopper, Nilaparvata lugens.

  2015cited by this paper
• Transcription profiling of 12 asian gypsy moth (Lymantria dispar) cytochrome P450 genes in response to insecticides.

  2014cited by this paper
• Identification of a novel cytochrome P450 gene, CYP321E1 from the diamondback moth, Plutella xylostella (L.) and RNA interference to evaluate its role in chlorantraniliprole resistance.

  2014cited by this paper
• Genome-wide analysis reveals the expansion of Cytochrome P450 genes associated with xenobiotic metabolism in rice striped stem borer, Chilo suppressalis.

  2014cited by this paper
• Multiple P450 genes: Identification, tissue-specific expression and their responses to insecticide treatments in the oriental fruit fly, Bactrocera dorsalis (Hendel) (Diptera: Tephritidea)

  2013cited by this paper
• Integrated analysis of cytochrome P450 gene superfamily in the red flour beetle, Tribolium castaneum

  2013cited by this paper
• Identification of cytochrome P450 monooxygenase genes and their expression profiles in cyhalothrin-treated Colorado potato beetle, Leptinotarsa decemlineata.

  2013cited by this paper
• Two cytochrome P450 genes are involved in imidacloprid resistance in field populations of the whitefly, Bemisia tabaci, in China.

  2013cited by this paper
• A Cytochrome P450 Conserved in Insects Is Involved in Cuticle Formation

  2012cited by this paper
• Antennal and behavioural responses of Heortia vitessoides females to host plant volatiles of Aquilaria sinensis

  2012cited by this paper
• Recent studies on insect hormone metabolic pathways mediated by cytochrome P450 enzymes.

  2012cited by this paper
• Arthropod CYPomes illustrate the tempo and mode in P450 evolution.

  2011cited by this paper
• MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods.

  2011cited by this paper
• Genome-wide analysis of cytochrome P450 monooxygenase genes in the silkworm, Bombyx mori.

  2011cited by this paper
• Trinity: reconstructing a full-length transcriptome without a genome from RNA-Seq data

  2011cited by this paper
• Genome-wide analysis of cytochrome P 450 monooxygenase genes in the silkworm , Bombyx mori

  2011cited by this paper
• Studies on the Synergistic Effects of Chlorantraniliprole and Beta-cypermethrin on Heortia vitessoides Moore

  2011cited by this paper
• Expression responses of nine cytochrome P450 genes to xenobiotics in the cotton bollworm Helicoverpa armigera

  2010cited by this paper
• Ecological Significance of Induction of Broad-Substrate Cytochrome P450s by Natural and Synthetic Inducers in Helicoverpa zea

  2009cited by this paper
• Co-up-regulation of three P450 genes in response to permethrin exposure in permethrin resistant house flies, Musca domestica

  2008cited by this paper
• Molecular mechanisms of metabolic resistance to synthetic and natural xenobiotics.

  2007cited by this paper
• Helicoverpa zea CYP6B8 and CYP321A1: different molecular solutions to the problem of metabolizing plant toxins and insecticides.

  2007cited by this paper
• Molecular evolution of the insect Halloween family of cytochrome P450s: phylogeny, gene organization and functional conservation.

  2007cited by this paper
• A deficit of detoxification enzymes: pesticide sensitivity and environmental response in the honeybee

  2006cited by this paper
• InterProScan: protein domains identifier

  2005cited by this paper
• Identification of cytochrome P450 and glutathione-S-transferase genes preferentially expressed in chemosensory organs of the swallowtail butterfly, Papilio xuthus L.

  2005cited by this paper
• Transcriptional suppression of cytochrome P450 genes by endogenous and exogenous chemicals.

  2004cited by this paper
• A new mathematical model for relative quantification in real-time RT-PCR.

  2001cited by this paper
• The cytochrome P450 gene superfamily in Drosophila melanogaster: annotation, intron-exon organization and phylogeny.

  2001cited by this paper
• Cytochrome P450 (CYP)9A1 in Heliothis virescens: the first member of a new CYP family.

  1997cited by this paper
• Cytochrome P-450 monooxygenases in insects.

  1989cited by this paper
• BIOINFORMATICS APPLICATIONS NOTE

  year unknowncited by this paper

• Comparative transcriptome analysis of leaf, stem, and root tissues of Semiliquidambar cathayensis reveals candidate genes involved in terpenoid biosynthesis

  2022cites this paper
• Comparative Transcriptome Analysis of Leaf, Stem, and Root Tissues of Semiliquidambar Cathayensis Hung T. Chang Reveals Candidate Genes Involved in Terpenoid Biosynthesis

  2022cites this paper
• Characterization of Dendrolimus houi Lajonquiere (Lepidoptera: Lasiocampidae) Transcriptome across All Life Stages

  2019cites this paper