Dynamics of activating and repressive histone modifications in Drosophila neural stem cell lineages and brain tumors

ABSTRACT During central nervous system development, spatiotemporal gene expression programs mediate specific lineage decisions to generate neuronal and glial cell types from neural stem cells (NSCs). However, little is known about the epigenetic landscape underlying these highly complex developmental events. Here, we perform ChIP-seq on distinct subtypes of Drosophila FACS-purified NSCs and their differentiated progeny to dissect the epigenetic changes accompanying the major lineage decisions in vivo. By analyzing active and repressive histone modifications, we show that stem cell identity genes are silenced during differentiation by loss of their activating marks and not via repressive histone modifications. Our analysis also uncovers a new set of genes specifically required for altering lineage patterns in type II neuroblasts (NBs), one of the two main Drosophila NSC identities. Finally, we demonstrate that this subtype specification in NBs, unlike NSC differentiation, requires Polycomb-group-mediated repression. Highlighted Article: A FACS-based method for sorting different cell types and their ChIP-seq have provided an in vivo dataset that reveals dynamic histone modifications during neuronal differentiation in Drosophila.

• Publication year

  2019

• Venue

  Development

• Publication date

  2019-01-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1242/dev.183400PMID 31748204PMCID PMC7055365
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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