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Screening of lignocellulolytic fungi for hydrolyzation of lignocellulosic materials in paddy straw for bioethanol production

M. Ghazali,N. I. Zainudin,N. A. Aziz,Muskhazli Mustafa

Published 2019 in Malaysian Journal of Microbiology

ABSTRACT

Aims: Paddy straw is known to have lignocellulosic materials such as cellulose and hemicellulose which can be readily converted into fermentable sugar for production of bioethanol via simultaneous saccharification and fermentation (SSF). In order to produce ethanol competently, the degradation of biomass by cellulase and highly ethanol-producing microorganism in fermentation process are necessarily needed. However, there is lacking in cellulose degrading organism in producing adequate amount of lignocellulosic enzyme. Therefore, the screening and selection for the best fungi to hydrolyze the lignocellulosic materials as well as forming consortium between two species of fungi has become the main focus. Methodology and results: Thirteen strains of fast-growing fungi were tested qualitatively for cellulase (congo red staining) and polyphenol oxidase (Bavendamm test). All tested strains displayed lignocellulolytic fungi characteristics. The selection was narrowed down by quantitative assay on endoglucanase, exoglucanase, β-glucosidase and xylanase and the highest cellulases enzyme producer were T. asperellum B1581 (3.93 U/mL endoglucanase; 2.37 U/mL exoglucanase; 3.00 IU/mL β-glucosidase; 54.87 U/mL xylanase), followed by A. niger B2484 (5.60 U/mL endoglucanase; 1.08 U/mL exoglucanase; 1.57 IU/mL β-glucosidase; 56.85 U/mL xylanase). In compatibility test, both T. asperellum B1581 and A. niger B2484 were inoculated on the same petri dish for 4 days and the interaction showed by the two species was mutual intermingling. Conclusions, significance and impact of study: Both Trichoderma asperellum B1581 and Aspergillus niger B2484 produced the highest cellulase enzyme. Since both strains can co-exist and produce enzymes that complete each other, a fungal consortium was suggested to increase the yield of sugars in saccharification process.

PUBLICATION RECORD

  • Publication year

    2019

  • Venue

    Malaysian Journal of Microbiology

  • Publication date

    2019-08-01

  • Fields of study

    Biology, Engineering, Environmental Science

  • Identifiers
    DOI 10.21161/MJM.180250
  • External record

    Open on Semantic Scholar

  • Source metadata

    Semantic Scholar

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