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Molecular subtyping and dna sequencing homology of Escherichia coli O157:H7 isolated from Aceh cattle

Mahdi Abrar,T. Ferasyi,Amiruddin,Fakhrurrazi,Erina,Razali,Mustafa Sabri,H. Abdullah,Zainuddin,A. Haris,Safika,Maryulia Dewi,Rina Aulia Barus

Published 2019 in IOP Conference Series: Earth and Environment

ABSTRACT

Escherichia coli (E. coli) O157:H7 is one of bacteria that recognized as pathogenic agent, both in animals and human. Several isolates of this bacteria were obtained from local livestock, such as in bali cattle and aceh cattle. However, the bacteria found in aceh cattle has not been confirmed through molecular technique. The current study was aimed to confirm the finding of E. coli strain O157:H7 in aceh cattle using molecular analysis. Three isolates of E. coli O157 (MA-35, MA-44, and MA-58) obtained from aceh cattle were extracted for the DNA using Presto™ Mini gDNA bacteria kit (Geneaid) through several processing stages of sample preparation, lysis, DNA binding, washing and elution. Then, it was continued with DNA Amplification Using Polimerase Chain Reaction (PCR). DNA sequencing homology was conducted using BLASTN program. Data of this study were analysed descriptively. The results showed that one of isolate (MA-58) was detected for the presence of genes of rfb (339 bp), fliC (401 bp), and stx2 (115 bp). Then, this isolate has a homology of 100% to 16 different strains of E. coli O157:H7 sequence in the GenBank. In conclusion, the molecular analysis of the local isolate of bacteria of MA-58 obtained from aceh cattle confirmed that it is E. coli O157:H7 and detected with the presence of genes of rfb, fliC, and stx2.

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