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Biodegradation of diethyl phthalate by Pseudomonas sp. BZD-33 isolated from active sludge

Z. Ke,Wu Xiangling,Che Jian,Chen Jia

Published 2019 in IOP Conference Series: Earth and Environment

ABSTRACT

Diethyl phthalate (DEP) is a common pollutant, which is widely used in industry. A strain of DEHP-degrading bacteria was isolated from activated sludge.According to the phylogeny of 16S rDNA sequence, BDZ-33 strain was identified as pseudomonas sp. Biodegradation of DEP by Pseudomonas sp. BZD-33 was investigated. The results show that the BZD - 33 degradation DEP optimal pH and temperature of 8.0 and 15 °C respectively. The degradation concentration ranged from 100-400 mg·L−1. The degradation efficiency and growth rate of the strain were higher under alkaline conditions than under acidic conditions. The best temperature below 20 °C. The degradation rate decreases with the increase of temperature. After 60 h of culture, the degradation rate of DEP wastewater with a concentration of 400 mg/L reached 90%. The results showed that BDZ-33 could efficiently degrade DEP, which might be a promising DEP repair pathway.

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