A novel serine protease from pseuderanthemum latifolium B. Hansen: Characterization and fibrino(geno)lytic activities

Abstract Protease (PPL) was isolated from Pseuderanthemum latifolium B. Hansen and had a molecular mass of 70 kDa. The N-terminal sequence of PPL showed 70-80% similarity with of subtilisin-like serine proteases from plants, but it did not show any sequence homology with known plant proteases. Serine protease inhibitors (PMSF, DFP) effectively blocked about 90% of PPL activity. PPL was highly activity at the pH range from 6 to 9 and temperatures from 50 °C to 80 °C, with an optimum at pH 7.0 and temperatures 70 °C. PPL had stability in a variety of pH, temperature, surfactant and oxidizing agents. PPL with concentration of 2.5 µg completely hydrolyzed the Aα-chain of fibrinogen within 5 min and hydrolyzed the Bβ and the γ-chain after 10 h. Fibrin also was strong hydrolyzed by PPL with concentration of 0.3 µg. Thus, PPL is a unique serine protease, which it had strong fibrino(geno)lytic activities. GRAPHICAL ABSTRACT

• Publication year

  2019

• Venue

  Natural Product Research

• Publication date

  2019-08-23

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1080/14786419.2019.1656626PMID 31441669
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Exploring hemostatic and thrombolytic potential of heynein - A cysteine protease from Ervatamia heyneana latex.

  2017cited by this paper
• Inhibitory actions of Pseuderanthemum palatiferum (Nees) Radlk. leaf ethanolic extract and its phytochemicals against carbohydrate-digesting enzymes

  2016cited by this paper
• Purification and biochemical properties of SDS-stable low molecular weight alkaline serine protease from Citrullus colocynthis

  2016influential reference
• Hypoglycemic activity in diabetic rats of stigmasterol and sitosterol-3-O--D-glucopyranoside isolated from Pseuderanthemum palatiferum (Nees) Radlk. leaf extract

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• Purification and Characterization of a Thermostable Caseinolytic Serine Protease from the Latex of Euphorbia heterophylla L.

  2015influential reference
• Anti-inflammatory Activity of Ethanol Extract from the Leaves of Pseuderanthemum palatiferum (Nees) Radlk.

  2013cited by this paper
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  2012influential reference
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  2012influential reference
• Lignans and Other Constituents from the Roots of the Vietnamese Medicinal Plant Pseuderanthemum palatiferum

  2011cited by this paper
• Antihypertensive effects of continuous oral administration of nattokinase and its fragments in spontaneously hypertensive rats.

  2011cited by this paper
• Hypotensive effect of the water extract of the leaves of Pseuderanthemum palatiferum

  2011cited by this paper
• Proteases as therapeutics.

  2011cited by this paper
• The Hag-protease-II is a fibrin(ogen)ase from Hippasa agelenoides spider venom gland extract: purification, characterization and its role in hemostasis.

  2011cited by this paper
• Hypoglycemic effect of a leaf extract of Pseuderanthemum palatiferum (Nees) Radlk.

  2010cited by this paper
• Purification and biochemical characterization of Eumiliin from Euphorbia milii var. hislopii latex.

  2010cited by this paper
• Hypoglycemic effect of a leaf extract of Pseuderanthemum palatiferum (Nees) Radlk. in normal and streptozotocin-induced diabetic rats.

  2010cited by this paper
• Acute Toxicity and Sub-acute Toxicity of Pseuderanthemun palatiferum (Nees) Radlk. Leaf Extract

  2010cited by this paper
• Benghalensin, a highly stable serine protease from the latex of medicinal plant Ficus benghalensis.

  2009cited by this paper
• A novel surfactant-stable alkaline serine-protease from a newly isolated Bacillus mojavensis A21. Purification and characterization

  2009cited by this paper
• Bromelain Inhibits Lipopolysaccharide-Induced Cytokine Production in Human THP-1 Monocytes via the Removal of CD14

  2008cited by this paper
• Purification and characterization of a 34-kDa, heat stable glycoprotein from Synadenium grantii latex: action on human fibrinogen and fibrin clot.

  2006cited by this paper
• Highly stable glycosylated serine protease from the medicinal plant Euphorbia milii.

  2006influential reference
• Pancreatic enzyme therapy and clinical outcomes in patients with cystic fibrosis.

  2005cited by this paper
• Purification and characterization of a subtilisin-like serine protease induced during the senescence of wheat leaves

  2003cited by this paper
• A subtilisin-like serine protease involved in the regulation of stomatal density and distribution in Arabidopsis thaliana.

  2000cited by this paper
• [The mechanisms of the curative action of systemic enzyme therapy].

  2000cited by this paper
• The treatment of paediatric burns using topical papaya.

  1999cited by this paper
• Purification and characterization of a protease from jackfruit latex

  1990influential reference
• Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes.

  1987cited by this paper
• [Toxicological study of nitrotoluenes: acute toxicity and sub-acute toxicity].

  1980cited by this paper
• Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

  1970cited by this paper
• Pancreatic enzyme therapy.

  1970cited by this paper
• A protein sequenator.

  1967cited by this paper
• The fibrin plate method for estimating fibrinolytic activity.

  1952cited by this paper
• Protein measurement with the Folin phenol reagent.

  1951influential reference

• Plant Latex Proteases in Hemostasis: Beyond Thrombin-like Activity

  2025cites this paper
• Diverse origins of fibrinolytic enzymes: A comprehensive review

  2024cites this paper