Hexamine cobalt chloride promotes intermolecular ligation of blunt end DNA fragments by T4 DNA ligase.

No abstract is available for this paper.

• Publication year

  1985

• Venue

  Nucleic Acids Research

• Publication date

  1985-03-25

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1093/NAR/13.6.1997PMID 4000951PMCID PMC341130
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Molecular Cloning: A Laboratory Manual

  2001cited by this paper
• Isolation of altered recA polypeptides and interaction with ATP and DNA.

  1985cited by this paper
• DNA binding protein from ovaries of the frog, Xenopus laevis which promotes concatenation of linear DNA.

  1984cited by this paper
• Macromolecular crowding allows blunt-end ligation by DNA ligases from rat liver or Escherichia coli.

  1983cited by this paper
• Isolation and characterization of the recA gene of Rhizobium meliloti

  1983cited by this paper
• Polymer-stimulated ligation: enhanced blunt- or cohesive-end ligation of DNA or deoxyribooligonucleotides by T4 DNA ligase in polymer solutions.

  1983cited by this paper
• Conversion of B DNA between solution and fiber conformations.

  1981cited by this paper
• Temperature dependence of the joining by T4 DNA ligase of termini produced by type II restriction endonucleases

  1981cited by this paper
• Cation-induced toroidal condensation of DNA studies with Co3+(NH3)6.

  1980cited by this paper
• DNA-joining enzymes: a review.

  1979cited by this paper
• Counterion-induced condesation of deoxyribonucleic acid. a light-scattering study.

  1979cited by this paper
• The molecular theory of polyelectrolyte solutions with applications to the electrostatic properties of polynucleotides

  1978cited by this paper
• DNA condensation with polyamines I. Spectroscopic studies.

  1978cited by this paper
• Use of the T4 polynucleotide ligase in the joining of flush-ended DNA segments generated by restriction endonucleases.

  1978cited by this paper
• Ligation of EcoRI endonuclease-generated DNA fragments into linear and circular structures.

  1975cited by this paper
• DNA Ligase: Structure, Mechanism, and Function

  1974cited by this paper
• Susceptibility of the phiX-like phages G4 and G14 to R-EcoRi endonuclease.

  1974cited by this paper
• Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.

  1972cited by this paper
• CXII. Total synthesis of the structural gene for an alanine transfer RNA from yeast. Enzymic joining of the chemically synthesized polydeoxynucleotides to form the DNA duplex representing nucleotide sequence 1 to 20.

  1972cited by this paper
• Enzymatic joining of polynucleotides. IX. A simple and rapid assay of polynucleotide joining (ligase) activity by measurement of circle formation from linear deoxyadenylate-deoxythymidylate copolymer.

  1970cited by this paper
• Enzymatic breakage and joining of deoxyribonucleic acid. VI. Further purification and properties of polynucleotide ligase from Escherichia coli infected with bacteriophage T4.

  1968cited by this paper

• Reducing Supply Chain Dependencies for Viral Genomic Surveillance: Get by with a Little HELP from Commercial Enzymes already in your Lab Freezer

  2025cites this paper
• m^6A-SAC-seq for quantitative whole transcriptome m^6A profiling

  2022cites this paper
• ZeBRα a universal, multi-fragment DNA-assembly-system with minimal hands-on time requirement

  2019cites this paper
• Ligation and Ligases.

  2019cites this paper
• XNA ligation using T4 DNA ligase in crowding conditions.

  2018cites this paper
• ffi cient DNA ligation by selective heating of DNA ligase with a radio frequency alternating magnetic fi

  2016cites this paper
• Efficient DNA ligation by selective heating of DNA ligase with a radio frequency alternating magnetic field

  2016cites this paper
• Rad51 and RecA juxtapose dsDNA ends ready for DNA ligase-catalyzed end-joining under recombinase-suppressive conditions

  2016cites this paper
• Why Johnny can't clone: Common pitfalls and not so common solutions.

  2015cites this paper
• In situ extraction of RNA from marine-derived fungi associated with the marine sponge, Haliclona simulans

  2012influential citation
• DESIGN AND CHARACTERIZATION OF A SYSTEM TO TEST SYNTHETIC DNA SEQUENCES FOR TELOMERE FUNCTION IN THE YEAST

  2010cites this paper
• Efficient ligation and cloning of DNA fragments with 2-bp overhangs.

  2010cites this paper
• End-joining long nucleic acid polymers

  2008cites this paper
• A noncommercial polymerase chain reaction-based method to approach one hundred percent recombinant clone selection efficiency.

  2008cites this paper
• A new 10-min ligation method using a modified buffer system with a very low amount of T4 DNA ligase: the “Coffee Break Ligation” technique

  2007cites this paper
• Concentration-dependent organization of DNA by the dinoflagellate histone-like protein HCc3

  2007cites this paper
• Molecular characterization of the putative oncogene myeov

  2005cites this paper
• Trisomy: a useful adjunct to RFLP mapping in pea

  2004cites this paper
• Clone bank and physical and genetic map of potato chloroplast DNA

  2004cites this paper
• Principles of Genetic Engineering for Escherichia coli

  2001cites this paper
• Subunits of Escherichia coli DNA polymerase III holoenzyme

  2000cites this paper
• Maize chlorotic mottle machlomovirus expresses its coat protein from a 1.47-kb subgenomic RNA and makes a 0.34-kb subgenomic RNA.

  2000cites this paper
• Increased cloning efficiency by cycle restriction−ligation (CRL)

  1997cites this paper
• Bacterial production of transdihydroxycyclohexadiene carboxylates by metabolic pathway engineering.

  1996cites this paper
• Increased cloning efficiency by temperature-cycle ligation.

  1996cites this paper
• Microbial production of specifically ring-13C-labelled 4-hydroxybenzoic acid

  1995cites this paper
• Salvaging recombinants from low-efficiency ligase reactions for more efficient subcloning.

  1995cites this paper
• Protection of megabase DNA from shearing.

  1995cites this paper
• Nonenzymatic plasmid ligation mediated by minor groove-binding molecules.

  1994cites this paper
• Cloning of cDNA for rat pro alpha 1(III) collagen mRNA. Different expression patterns of type I and type III collagen and fibronectin genes in experimental granulation tissue.

  1994cites this paper
• Glycine 85 of the trp-repressor of E. coli is important in forming the hydrophobic tryptophan binding pocket: experimental and computational approaches.

  1994cites this paper
• Molecular cloning of cDNA encoding alliinase from onion (Allium cepa L.)

  1993cites this paper
• DNA and RNA ligases (EC 6.5.1.1, EC 6.5.1.2, and EC 6.5.1.3).

  1993cites this paper
• Transcripts of a maize chlorotic mottle virus cDNA clone replicate in maize protoplasts and infect maize plants.

  1993cites this paper
• Turbo cloning: a fast, efficient method for cloning PCR products and other blunt-ended DNA fragments into plasmids.

  1993cites this paper
• Ultrasonic ligation for rapid high-efficiency subcloning in plasmid vectors.

  1993cites this paper
• Regulatory aspects of esterase 6 activity variation in sibling Drosophila species

  1993cites this paper
• Recombination and UV resistance of Escherichia coli with the cloned recA and recBCD genes of Serratia marcescens and Proteus mirabilis: evidence for an advantage of intraspecies combination of P. mirabilis RecA protein and RecBCD enzyme.

  1992cites this paper
• Cloning and sequencing of the gene for the cytoplasmic inorganic pyrophosphatase from the thermoacidophilic archaebacterium Thermoplasma acidophilum.

  1992cites this paper
• A nucleic acid-based test for detection of Fasciola hepatica.

  1992cites this paper
• Intraspecific mitochondrial DNA variation in the migratory grasshopper, Melanoplus sanguinipes

  1992cites this paper
• Ligation with T4 RNA ligase of an oligodeoxyribonucleotide to covalently-linked cross-sectional base-pair analogues of short, normal, and long dimensions.

  1991cites this paper
• The recA-recBCD dependent recombination pathways of Serratia marcescens and Proteus mirabilis in Escherichia coli: functions of hybrid enzymes and hybrid pathways.

  1991cites this paper
• Defective viral DNA in Epstein-Barr virus-associated oral hairy leukoplakia

  1990cites this paper
• Phosphoenolpyruvate carboxylase prevalent in maize roots: isolation of a cDNA clone and its use for analyses of the gene and gene expression.

  1990cites this paper
• 65-kilodalton protein phosphorylated by interleukin 2 stimulation bears two putative actin-binding sites and two calcium-binding sites.

  1990cites this paper
• Nonessential region of bacteriophage P4: DNA sequence, transcription, gene products, and functions

  1990cites this paper
• Regions of the Streptococcus sobrinus spaA gene encoding major determinants of antigen I

  1990cites this paper
• Mechanistic studies of ionizing radiation and oxidative mutagenesis: genetic effects of a single 8-hydroxyguanine (7-hydro-8-oxoguanine) residue inserted at a unique site in a viral genome.

  1990cites this paper
• Isolation and expression of a plastid α chaperonin cDNA sequence from Triticum aestivum.

  1989cites this paper
• Variable region primary structures of a high affinity anti-fluorescein immunoglobulin M cryoglobulin exhibiting oxazolone cross-reactivity.

  1989cites this paper
• Farnesyl diphosphate synthetase. Molecular cloning, sequence, and expression of an essential gene from Saccharomyces cerevisiae.

  1989cites this paper
• Functional analyses of Proteus mirabilis wild‐type and mutant RecBCD enzymes in Escherichia coli reveal a new mutant phenotype

  1989cites this paper
• Genomic sequencing and methylation analysis by ligation mediated PCR.

  1989cites this paper
• Effect of histone H1, poly(ethyleneglycol) and DNA concentration on intermolecular and intramolecular ligation by T4 DNA ligase.

  1988cites this paper
• The chloroplast-located glutamine synthetase of Phaseolus vulgaris L.: nucleotide sequence, expression in different organs and uptake into isolated chloroplasts

  1988cites this paper
• Chloroplast DNA differences in the genus Oenothera subsection Munzia: a short direct repeat resembling the lambda chromosomal attachment site occurs as a deletion/insertion within an intron of an NADH-dehydrogenase gene

  1988cites this paper
• Determination of the single polyadenylation site of the human pro alpha 1(II) collagen gene.

  1987cites this paper
• Novel type of plasmid-borne resistance to trimethoprim

  1987cites this paper
• Rapid and efficient method for cloning of blunt-ended DNA fragments.

  1987cites this paper
• [38] Adaptors, linkers, and methylation

  1987cites this paper
• Construction of cDNA libraries by blunt-end ligation: high-frequency cloning of long cDNAs from filamentous fungi.

  1987cites this paper
• Adaptors, linkers, and methylation.

  1987cites this paper
• RecA protein filaments can juxtapose DNA ends: an activity that may reflect a function in DNA repair.

  1986cites this paper
• Ligation of single-stranded oligodeoxyribonucleotides by T4 RNA ligase.

  1986cites this paper
• HCC ligation: rapid and specific DNA construction with blunt ended DNA fragments.

  1986cites this paper
• In vitro mutagenesis.

  1985cites this paper