Induction of Necrosis in Human Macrophage Cell Lines by Corynebacterium diphtheriae and Corynebacterium ulcerans Strains Isolated from Fatal Cases of Systemic Infections

When infecting a human host, Corynebacterium diphtheriae and Corynebacterium ulcerans are able to impair macrophage maturation and induce cell death. However, the underlying molecular mechanisms are not well understood. As a framework for this project, a combination of fluorescence microscopy, cytotoxicity assays, live cell imaging, and fluorescence-activated cell sorting was applied to understand the pathogenicity of two Corynebacterium strains isolated from fatal cases of systemic infections. The results showed a clear cytotoxic effect of the bacteria. The observed survival of the pathogens in macrophages and, subsequent, necrotic lysis of cells may be mechanisms explaining dissemination of C. diphtheriae and C. ulcerans to distant organs in the body.

• Publication year

  2019

• Venue

  International Journal of Molecular Sciences

• Publication date

  2019-08-22

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.3390/ijms20174109PMID 31443569PMCID 6747468
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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  2015cited by this paper
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  2015cited by this paper
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  2014cited by this paper
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  2012cited by this paper
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  2012cited by this paper
• Comparative analysis of two complete Corynebacterium ulcerans genomes and detection of candidate virulence factors

  2011cited by this paper
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  2010cited by this paper
• Non‐opsonic phagocytosis of homologous non‐toxigenic and toxigenic Corynebacterium diphtheriae strains by human U‐937 macrophages

  2010influential reference
• Potential pathogenic role of aggregative-adhering Corynebacterium diphtheriae of different clonal groups in endocarditis.

  2008cited by this paper
• First detection of Corynebacterium ulcerans producing a diphtheria-like toxin in a case of human with pulmonary infection in the Rio de Janeiro metropolitan area, Brazil.

  2008cited by this paper
• Expression of phospholipase D, the major virulence factor of Corynebacterium pseudotuberculosis, is regulated by multiple environmental factors and plays a role in macrophage death.

  2007cited by this paper
• Plasmid Vectors for Testing In Vivo Promoter Activities in Corynebacterium glutamicum and Rhodococcus erythropolis

  2007cited by this paper
• Multiple roles of cord factor in the pathogenesis of primary, secondary, and cavitary tuberculosis, including a revised description of the pathology of secondary disease.

  2006cited by this paper
• Phospholipase D: enzymology, mechanisms of regulation, and function.

  1997cited by this paper
• Targeted mutagenesis of the phospholipase D gene results in decreased virulence of Corynebacterium pseudotuberculosis

  1994cited by this paper
• Rational attenuation of Corynebacterium pseudotuberculosis: potential cheesy gland vaccine and live delivery vehicle

  1992cited by this paper
• Patterns of cell death.

  1988cited by this paper
• Establishment and characterization of a human acute monocytic leukemia cell line (THP‐1)

  1980cited by this paper
• TAXONOMICAL STUDIES ON GLUTAMIC ACID-PRODUCING BACTERIA

  1967cited by this paper
• The Journal of Laryngology and Rhinology

  1887cited by this paper
• Corynebacterium ulcerans : A pathogenic microorganism resembling Corynebacterium diphtheria

  year unknowncited by this paper

• Proteome Analysis of Corynebacterium diphtheriae-Macrophage Interaction.

  2025cites this paper
• Genome-wide high-throughput transposon mutagenesis unveils key factors for acidic pH adaptation of Corynebacterium diphtheriae

  2025cites this paper
• The cellular model for Alzheimer's disease research: PC12 cells

  2023cites this paper
• Pathogene Corynebakterien: erfolgreiche Kolonisierer von Mensch und Tier

  2023cites this paper
• Multi-Omics of Corynebacterium Pseudotuberculosis 12CS0282 and an In Silico Reverse Vaccinology Approach Reveal Novel Vaccine and Drug Targets

  2022cites this paper
• Interactions between the Re-Emerging Pathogen Corynebacterium diphtheriae and Host Cells

  2022cites this paper
• Host–Pathogen Interaction 3.0

  2022cites this paper
• Corynebacterium spp. - problematic pathogens of the human respiratory tract (review of literature).

  2021cites this paper
• Live cell imaging of macrophage/bacterium interaction demonstrates cell lysis induced by Corynebacterium diphtheriae and Corynebacterium ulcerans

  2019cites this paper