Ferric Uptake Regulator (FurA) is required for Acidovorax citrulli virulence on watermelon.

Acidovorax citrulli is the causal agent of bacterial fruit blotch (BFB), a serious threat to commercial watermelon and melon crop production worldwide. Ferric Uptake Regulator (Fur) is a global transcription factor that affects a number of virulence-related functions in phytopathogenic bacteria; however, the role of furA has not been determined for A. citrulli. Hence, we constructed a furA deletion mutant of A. citrulli strain xlj12 to investigate the the role of the gene in siderophore production, concentration of intracellular Fe2+, bacterial sensitivity to hydrogen peroxide, biofilm formation, swimming motility, hypersensitive response induction and virulence. The A.citrulli furA deletion mutant displayed increased siderophore production, increased intracellular Fe2+ concentration and increased sensitivity to hydrogen peroxide. In contrast, biofilm formation, swimming motility, and virulence on watermelon seedlings were significantly reduced in the furA mutant. In accordance with the phenotypic results, the expression levels of bfrA and bfrB, that encode bacterioferritin; sodB that encodes iron/manganese superoxide dismutase; fliS that encodes a flagellar protein; hrcN that encodes the type III secretion system (T3SS) ATPase; and hrcC that encodes the T3SS outer membrane ring protein were significantly down-regulated in the A. citrulli furA deletion mutant. In addition, the expression of feo-related genes, feoA, feoB, was significantly up-regulated in the furA mutant. Overall, these results indicated that in A. citrulli FurA contributes to the regulation of the iron balance system, and affects a variety of virulence-related factors.

• Publication year

  2019

• Venue

  Phytopathology

• Publication date

  2019-11-05

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1094/PHYTO-05-19-0172-RPMID 31454303
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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