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Average conformation of branched-chain lipid PGP-Me that accounts for the thermal stability and high-salinity resistance of archaeal membranes.

Masaki Yamagami,H. Tsuchikawa,Jin Cui,Yuichi Umegawa,Yusuke Miyazaki,Sangjae Seo,W. Shinoda,M. Murata

Published 2019 in Biochemistry

ABSTRACT

The average conformation of the methyl-branched chains of archaeal lipid, phosphatidyl glycerophosphate methyl ester (PGP-Me), was examined in hydrated bilayer membrane base on the solid state NMR of enantioselectively 2H-labeled compounds that were totally synthesized for the first time in this study. The NMR results in combination with molecular dynamics simulations revealed that the PGP-Me chain appeared to behave differently from that of usual membrane lipids such as dipalmitoylphosphatidylcholine (DMPC). The C-C bonds of the PGP-Me chain adopt alternative parallel and tilted orientations to the membrane normal as opposed to a DMPC chain where all of the C-C bonds tilt in the same way on average. This characteristic orientation causes the intertwining of the middle part of PGP-Me chains, which plays an important role in the excellent thermal and high-salinity stabilities of archaeal lipid bilayers and membrane proteins.

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