Antiviral activity of seselin from Aegle marmelos against nuclear polyhedrosis virus infection in the larvae of silkworm, Bombyx mori.

ETHNOPHARMACOLOGICAL RELEVANCE Traditional Indian medicine has utilized Aegle marmelos (L.) Corr. commonly called as bael in several indigenous systems against various diseases. Bioactive components isolated from various plant parts of A. marmelos were used in ethno-medicine. More precisely they are known for its antiviral property against various human and animal viruses. AIM OF THE STUDY The study was conducted to investigate the antiviral activity of A. marmelos against Bombyx mori nucleopolyhedrovirus (BmNPV). MATERIALS AND METHODS Among the various crude extracts tested, hexane extracts of leaves of A. marmelos with promising anti-BmNPV activity was subjected to bioactivity guided fractionation based on column chromatography. Out of 40 fractions obtained from the fractionation, fractions showing similar TLC profiles were pooled into 14 fractions. A fraction with potential activity was used to purify a molecule with anti-BmNPV activity. This molecule was characterized through structural and functional analyses. RESULTS The functionally and structurally characterized molecule in the fraction with prospective anti-BmNPV activity revealed a single crystal compound 'seselin' (8, 8-dimethyl pyrido oxazine-2-one). CONCLUSION It is therefore understood that this seselin compound could be used as a natural medicine for the management of NPV infection in the silkworm larvae under commercial conditions after suitable field evaluations.

• Publication year

  2019

• Venue

  Journal of Ethnopharmacology

• Publication date

  2019-12-05

• Fields of study

  Biology, Medicine, Chemistry, Environmental Science

• Identifiers
  DOI 10.1016/j.jep.2019.112155PMID 31449858
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Structure elucidation and DNA binding specificity of natural compounds from Cassia siamea leaves: A biophysical approach.

  2016cited by this paper
• Curative Effect of Ethanolic Plant Extractives Against Beauveria bassiana Infection in Silkworm , Bombyx mori L . : Histopathological Observations on Midgut

  2015cited by this paper
• Larvicidal activity of leaf extracts and seselin from Clausena anisata (Rutaceae) against Aedes aegypti

  2015cited by this paper
• Therapeutic potential of Aegle marmelos (L.)-An overview

  2014cited by this paper
• Isolation of seselin from Clausena anisata (Rutaceae) leaves and its effects on the feeding and development of Lucilia cuprina larvae may explain its use in ethnoveterinary medicine.

  2013cited by this paper
• Effect of medicinal botanical extracts on survivability of silkworm Bombyx mori (PM × CSR2)

  2012cited by this paper
• Antiviral Action of Certain Medicinal Plants against AmCPV and their Effect on Cellular and Biochemical Changes in Tasar Silkworm, Antheraea mylitta D.

  2012cited by this paper
• Dimorphisim in nuclear polyhedrosis virus (BmNPV) (Family: baculoviridae) causing ‘grasserie’ disease in silkworm (Bombyx mori L.): Light and electron microscopy and protein profile

  2012cited by this paper
• EFFECT OF BOTANICAL TREATMENT TO BM NPV POLYHEDRAL BODIES ON COCOON PARAMETERS OF SILKWORM B.MORI (PM x CSR2)

  2012cited by this paper
• An evaluation of the inhibitory effects against rotavirus infection of edible plant extracts

  2012cited by this paper
• Mutational Robustness of an RNA Virus Influences Sensitivity to Lethal Mutagenesis

  2011cited by this paper
• Aegle marmelos (Linn.) Correa: A potential source of Phytomedicine

  2011cited by this paper
• Autographa californica multiple nucleopolyhedrovirus ODV-E56 is a per os infectivity factor, but is not essential for binding and fusion of occlusion-derived virus to the host midgut.

  2011cited by this paper
• Botanicals against Nuclear Polyhedrosis Virus infecting three breeds of mulberry silkworm, Bombyx mori L.

  2010cited by this paper
• Hematological, biochemical and histopathological studies on marsh frog, Rana ridibunda, naturally infected with Waltonella duboisi.

  2010cited by this paper
• Screening of Medicinal Plants Against the Infection of Antheraea mylitta Cytoplasdmic Polyhedrosis Virus (AmCPV) in Tropical Tasar Silkworm, Antheraea mylitta Drury

  2010cited by this paper
• Differential ammonia metabolism in Aedes aegypti fat body and midgut tissues.

  2010cited by this paper
• Grasserie disease incidence on silkworm and development of botanical based management strategy.

  2010cited by this paper
• Anti-Herpetic Activity of Callissia fragrans and Simmondsia chinensis Leaf Extracts In Vitro

  2010cited by this paper
• Autographa californica Multiple Nucleopolyhedrovirus Core Gene ac96 Encodes a Per Os Infectivity Factor (pif-4)

  2009cited by this paper
• Cytopathology of Bombyx mori (Lepidoptera: Bombycidae) silk gland caused by multiple nucleopolyhedrovirus.

  2009cited by this paper
• A silkworm baculovirus model for assessing the therapeutic effects of antiviral compounds: characterization and application to the isolation of antivirals from traditional medicines.

  2008cited by this paper
• Seselin from Plumbago zeylanica inhibits phytohemagglutinin (PHA)-stimulated cell proliferation in human peripheral blood mononuclear cells.

  2008cited by this paper
• Efficacy of aqueous extracts of some botanicals against grasserie disease of silkworm, Bombyx mori L.

  2007cited by this paper
• Molecular biology of the baculovirus occlusion-derived virus envelope.

  2007cited by this paper
• Biochemical changes in haemolymph of silkworm larvae due to pyriproxyfen residue

  2007cited by this paper
• Screening the antiviral activity of Indian medicinal plants against white spot syndrome virus in shrimp

  2007cited by this paper
• Antifungal activity of seselin in protecting stored maize from Aspergillus flavus

  2007cited by this paper
• CASTp: computed atlas of surface topography of proteins with structural and topographical mapping of functionally annotated residues

  2006cited by this paper
• Antinociceptive activity of the pyranocoumarin seselin in mice.

  2006cited by this paper
• Efficacy of botanicals against grasserie disease of silkworm, Bombyx mori L

  2006cited by this paper
• Antinociceptive activity of the pyranocoumarin seselin in mice

  2006cited by this paper
• A sensitive bioassay for a nucleopolyhedrovirus using silkworms, Bombyx mori L., previously treated with a chitin synthesis inhibitor

  2005cited by this paper
• Turmeric and curcumin: Biological actions and medicinal applications

  2004cited by this paper
• CASTp: Computed Atlas of Surface Topography of proteins

  2003cited by this paper
• Effect of Systemic Fungicide on Total Hemocyte Count and Hemolymph Biochemical Changes in Silkworm, Bombyx mori L., infected with Beau-veria bassiana

  2002cited by this paper
• In vitro antiviral activity of bael (Aegle marmelos Corr) upon human coxsackieviruses B1-B6.

  2002cited by this paper
• Silkworm genomics - progress and prospects

  2002cited by this paper
• Haemocyte counts in different breeds of silkworm, Bombyx mori L. and their changes during the progressive infection of BmNPV

  2001cited by this paper
• The broad-spectrum antiviral ribonucleoside ribavirin is an RNA virus mutagen

  2000cited by this paper
• Efficacy of bed disinfectants and botanicals against grasserie disease of silkworm, Bombyx mori L.

  2000cited by this paper
• Python: a programming language for software integration and development.

  1999cited by this paper
• Introduction to Biostatistics

  1996cited by this paper
• LIGPLOT: a program to generate schematic diagrams of protein-ligand interactions.

  1995cited by this paper
• 3′,4′-Di-o-(-)-camphanoyl-(+)-ciskhellactone and related compounds: A. new class of potent anti-HIV agents

  1994cited by this paper
• Biological activity of some coumarins from Sri Lankan Rutaceae.

  1994cited by this paper
• Biochemical changes in the haemolymph of the silkworm, Bombyx mori L., during the progressive infection of nuclear polyhedrosis virus (BmNPV)

  1994cited by this paper
• Acridone alkaloids and coumarins from Pleiospermium alatum

  1990cited by this paper
• The occurrence and control of the silkworm diseases.

  1990cited by this paper
• Leaf phenolic inhibition of gypsy moth nuclear polyhedrosis virus Role of polyhedral inclusion body aggregation

  1990cited by this paper
• Isolation of Two Ovicidal Substances against Two-spotted Spider Mite, Tetranychus urticae Koch, from Skimmia repens Nakai

  1985cited by this paper
• Some alkaloids from aegle marmelos

  1983cited by this paper
• New alkaloids from Aegle marmelos

  1978cited by this paper
• The silkworm : an important laboratory tool

  1978cited by this paper
• Methods in immunology

  1977cited by this paper
• FACTORS AFFECTING VIRUS INFECTION OF INSECT GUT TISSUE

  1975cited by this paper
• The structural proteins and RNA components of a cytoplasmic polyhedrosis virus from Nymphalis io (Lepidoptera: Nymphalidae).

  1974cited by this paper
• Effects of Seselin and Coumarin on Growth, Indoleacetic Acid Oxidase, and Peroxidase, with Special Reference to Cucumber (Cucumis sativa L.) Radicles.

  1971cited by this paper
• Duration of infectivity of the virus of silkworm jaundice.

  1954cited by this paper
• Protein measurement with the Folin phenol reagent.

  1951cited by this paper

• High-throughput screening identifies paullinic acid as an antiviral agent against Bombyx mori nucleopolyhedrovirus.

  2025cites this paper
• Antiviral potential and mode of action of Metarhizium pinghaense 15R-derived polysaccharide against baculovirus.

  2025cites this paper
• Coumarins from Rutaceae: Chemical diversity and biological activities.

  2023cites this paper
• Oral administration of Zingiber officinale and Aegle marmelos extracts enhances growth and immune functions of the shrimp Penaeus monodon against the white spot syndrome virus (WSSV)

  2023cites this paper
• Crucial Role of the Ca2+/CN Signaling Pathway in the Antifungal Activity of Seselin against Botrytis cinerea.

  2023cites this paper
• The Potential Biological Roles of Circular RNAs in the Immune Systems of Insects to Pathogen Invasion

  2023cites this paper
• Computational Approach to Combat COVID-19 Infection: Emerging Tool for Accelerating Drug Research.

  2022cites this paper
• Ethno-Medical Informations from Coastal Odisha, a Review

  2022cites this paper
• Relationship Between Phylogenetic of Apium and Foeniculum Plants from Central Java, Indonesia, and Their Secondary Metabolites Potency against COVID-19 Protease

  2022cites this paper
• Evolution of extraction technique for the separation of bioactive compounds from Aegle marmelos

  2022cites this paper
• Plant lectins as prospective antiviral biomolecules in the search for COVID-19 eradication strategies

  2021cites this paper
• Phytochemicals from Plant Foods as Potential Source of Antiviral Agents: An Overview

  2021cites this paper
• PHYTOCHEMICAL ANALYSIS OF SELECTED INDIAN MEDICINAL PLANTS TO KNOW THE ANTIVIRIAL POTENTIAL AGAINST NOVEL CORONAVIRUS

  2021cites this paper
• Impacts of Aegle marmelos fruit extract as a medicinal herb on growth performance, antioxidant and immune responses, digestive enzymes, and disease resistance against Streptococcus agalactiae in Nile tilapia (Oreochromis niloticus).

  2021cites this paper
• Advances in the Arms Race Between Silkworm and Baculovirus

  2021cites this paper
• Inhibition of multiple SARS-CoV-2 proteins by an antiviral biomolecule, seselin from Aegle marmelos deciphered using molecular docking analysis

  2021cites this paper
• In Silico Exploration of Phytoconstituents From Phyllanthus emblica and Aegle marmelos as Potential Therapeutics Against SARS-CoV-2 RdRp

  2021cites this paper
• Physiochemical characterization and anti-carbapenemase activity of chitosan nanoparticles loaded Aegle marmelos essential oil against K. pneumoniae through DNA fragmentation assay

  2021cites this paper
• Inhibition of multiple SARS-CoV-2 proteins by an antiviral biomolecule, seselin from Aegle marmelos deciphered using molecular docking analysis

  2020cites this paper
• Phytochemicals and antiviral properties of five dominant medicinal plant species in Bankura district, West Bengal: An overview

  2020cites this paper
• Expression profile analysis of circular RNAs in BmN cells (Bombyx mori) upon BmNPV infection.

  2020cites this paper