Comprehensive systems biology analysis of a 7-month cigarette smoke inhalation study in C57BL/6 mice

Smoking of combustible cigarettes has a major impact on human health. Using a systems toxicology approach in a model of chronic obstructive pulmonary disease (C57BL/6 mice), we assessed the health consequences in mice of an aerosol derived from a prototype modified risk tobacco product (pMRTP) as compared to conventional cigarettes. We investigated physiological and histological endpoints in parallel with transcriptomics, lipidomics, and proteomics profiles in mice exposed to a reference cigarette (3R4F) smoke or a pMRTP aerosol for up to 7 months. We also included a cessation group and a switching-to-pMRTP group (after 2 months of 3R4F exposure) in addition to the control (fresh air-exposed) group, to understand the potential risk reduction of switching to pMRTP compared with continuous 3R4F exposure and cessation. The present manuscript describes the study design, setup, and implementation, as well as the generation, processing, and quality control analysis of the toxicology and ‘omics’ datasets that are accessible in public repositories for further analyses. Design Type(s) parallel group design • time series design • in vivo design • replicate design • injury design Measurement Type(s) mass • blood chemistry measurement • pulmonary function measurement • abnormal respiratory system physiology • Histopathologic Examination • transcription profiling assay • metabolite profiling • protein expression profiling Technology Type(s) weight scale • hematology • body plethysmography • bronchial alveolar lavage • histology • microarray platform • mass spectrometry assay Factor Type(s) group assignment • Exposure Duration • tissue specimen Sample Characteristic(s) Mus musculus • lung • nasal cavity epithelium • blood • multicellular organism • liver Design Type(s) parallel group design • time series design • in vivo design • replicate design • injury design Measurement Type(s) mass • blood chemistry measurement • pulmonary function measurement • abnormal respiratory system physiology • Histopathologic Examination • transcription profiling assay • metabolite profiling • protein expression profiling Technology Type(s) weight scale • hematology • body plethysmography • bronchial alveolar lavage • histology • microarray platform • mass spectrometry assay Factor Type(s) group assignment • Exposure Duration • tissue specimen Sample Characteristic(s) Mus musculus • lung • nasal cavity epithelium • blood • multicellular organism • liver Machine-accessible metadata file describing the reported data (ISA-Tab format)

• Publication year

  2016

• Venue

  Scientific Data

• Publication date

  2016-01-05

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1038/sdata.2015.77PMID 26731301PMCID 4700839
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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