AtSec62 is critical for plant development and is involved in ER‐phagy in Arabidopsis thaliana

Abstract The endoplasmic reticulum (ER) is the major site for protein folding in eukaryotic cells. ER homeostasis is essential for the development of an organism, whereby the unfolded protein response (UPR) within the ER is precisely regulated. ER‐phagy is a newly identified selective autophagic pathway for removal of misfolded or unfolded proteins within the ER in mammalian cells. Sec62, a component of the translocon complex, was recently characterized as an ER‐phagy receptor during the ER stress recovery phase in mammals. In this study, we demonstrated that the Arabidopsis Sec62 (AtSec62) is required for plant development and might function as an ER‐phagy receptor in plants. We showed that AtSec62 is an ER‐localized membrane protein with three transmembrane domains (TMDs) with its C‐terminus facing to the ER lumen. AtSec62 is required for plant development because atsec62 mutants display impaired vegetative growth, abnormal pollen and decreased fertility. atsec62 mutants are sensitive towards tunicamycin (TM)‐induced ER stress, whereas overexpression of AtSec62 subsequently enhances stress tolerance during the ER stress recovery phase. Moreover, YFP‐AtSec62 colocalizes with the autophagosome marker mCh‐Atg8e in ring‐like structures upon ER stress induction. Taken together, these data provide evidence for the pivotal roles of AtSec62 in plant development and ER‐phagy.

• Publication year

  2019

• Venue

  Journal of Integrative Plant Biology

• Publication date

  2019-11-19

• Fields of study

  Biology, Medicine, Chemistry, Environmental Science

• Identifiers
  DOI 10.1111/jipb.12872PMID 31483571PMCID 7028082
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• ATG5 and ATG7 induced autophagy interplays with UPR via PERK signaling

  2019cited by this paper
• Basic-leucine zipper 17 and Hmg-CoA reductase degradation 3A are involved in salt acclimation memory in Arabidopsis.

  2019cited by this paper
• The ER luminal C-terminus of AtSec62 is critical for male fertility and plant growth in Arabidopsis thaliana.

  2019cited by this paper
• A Functional Unfolded Protein Response Is Required for Normal Vegetative Development1[OPEN]

  2019cited by this paper
• Plant G proteins interact with endoplasmic reticulum luminal protein receptors to regulate endoplasmic reticulum retrieval.

  2018influential reference
• Endoplasmic reticulum turnover: ER-phagy and other flavors in selective and non-selective ER clearance

  2018influential reference
• ER-phagy requires Lnp1, a protein that stabilizes rearrangements of the ER network

  2018cited by this paper
• Signal motif-dependent ER export of the Qc-SNARE BET12 interacts with MEMB12 and affects PR1 trafficking in Arabidopsis

  2018cited by this paper
• Unraveling Field Crops Sensitivity to Heat Stress：Mechanisms, Approaches, and Future Prospects

  2018cited by this paper
• ER-phagy at a glance

  2018cited by this paper
• IRE1B degrades RNAs encoding proteins that interfere with the induction of autophagy by ER stress in Arabidopsis thaliana

  2018cited by this paper
• Unfolded protein response activation compensates endoplasmic reticulum-associated degradation deficiency in Arabidopsis.

  2017influential reference
• Loss of GET pathway orthologs in Arabidopsis thaliana causes root hair growth defects and affects SNARE abundance

  2017cited by this paper
• ATG9 regulates autophagosome progression from the endoplasmic reticulum in Arabidopsis

  2017cited by this paper
• Overexpressed BRH1, a RING finger gene, alters rosette leaf shape in Arabidopsis thaliana

  2017cited by this paper
• Arabidopsis PRK6 interacts specifically with AtRopGEF8/12 and induces depolarized growth of pollen tubes when overexpressed

  2017cited by this paper
• Unfolded protein response in pollen development and heat stress tolerance

  2016influential reference
• IRE1, a component of the unfolded protein response signaling pathway, protects pollen development in Arabidopsis from heat stress.

  2016cited by this paper
• Autophagy: A self-eating mechanism for maintaining cellular homeostasis

  2016cited by this paper
• Activation of autophagy by unfolded proteins during endoplasmic reticulum stress.

  2016influential reference
• The GET System Inserts the Tail-Anchored Protein, SYP72, into Endoplasmic Reticulum Membranes1[OPEN]

  2016cited by this paper
• Translocon component Sec62 acts in endoplasmic reticulum turnover during stress recovery

  2016influential reference
• Endoplasmic reticulum (ER) stress and the unfolded protein response (UPR) in plants

  2016cited by this paper
• Multiple BiP genes of Arabidopsis thaliana are required for male gametogenesis and pollen competitiveness.

  2014cited by this paper
• Cellular mechanisms of endoplasmic reticulum stress signaling in health and disease. 1. An overview.

  2014cited by this paper
• Interactions between autophagy receptors and ubiquitin-like proteins form the molecular basis for selective autophagy.

  2014cited by this paper
• A BAR-Domain Protein SH3P2, Which Binds to Phosphatidylinositol 3-Phosphate and ATG8, Regulates Autophagosome Formation in Arabidopsis[C][W]

  2013cited by this paper
• Comprehensive Protein-Based Artificial MicroRNA Screens for Effective Gene Silencing in Plants[W]

  2013cited by this paper
• AtTPR7 as part of the Arabidopsis Sec post-translocon

  2013cited by this paper
• Degradation of the Endoplasmic Reticulum by Autophagy during Endoplasmic Reticulum Stress in Arabidopsis[C][W]

  2012influential reference
• Different effects of Sec61α, Sec62 and Sec63 depletion on transport of polypeptides into the endoplasmic reticulum of mammalian cells

  2012cited by this paper
• Evidence for the involvement of the Arabidopsis SEC24A in male transmission

  2011cited by this paper
• The role of Atg proteins in autophagosome formation.

  2011cited by this paper
• Arabidopsis IRE1 catalyses unconventional splicing of bZIP60 mRNA to produce the active transcription factor

  2011influential reference
• The Unfolded Protein Response: From Stress Pathway to Homeostatic Regulation

  2011influential reference
• Function of IRE1 alpha in the placenta is essential for placental development and embryonic viability

  2009cited by this paper
• Overexpression of a Plant Reticulon Remodels the Lumen of the Cortical Endoplasmic Reticulum but Does not Perturb Protein Transport

  2008cited by this paper
• Arabidopsis thaliana has a set of J proteins in the endoplasmic reticulum that are conserved from yeast to animals and plants.

  2008influential reference
• Transient expression of fluorescent fusion proteins in protoplasts of suspension cultured cells

  2007cited by this paper
• An Endoplasmic Reticulum Stress Response in Arabidopsis Is Mediated by Proteolytic Processing and Nuclear Relocation of a Membrane-Associated Transcription Factor, bZIP28[W][OA]

  2007cited by this paper
• Intracellular signaling by the unfolded protein response.

  2006cited by this paper
• Highly Specific Gene Silencing by Artificial MicroRNAs in Arabidopsis[W][OA]

  2006cited by this paper
• Visualization of autophagy in Arabidopsis using the fluorescent dye monodansylcadaverine and a GFP-AtATG8e fusion protein.

  2005cited by this paper
• Quality control in the endoplasmic reticulum

  2003cited by this paper
• Rab2 GTPase Regulates Vesicle Trafficking between the Endoplasmic Reticulum and the Golgi Bodies and Is Important to Pollen Tube Growth Online version contains Web-only data. Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.000836.

  2002cited by this paper
• Mammalian Sec61 Is Associated with Sec62 and Sec63*

  2000influential reference
• Molecular Cloning and Further Characterization of a Probable Plant Vacuolar Sorting Receptor

  1997cited by this paper
• Protein folding in the cell

  1992cited by this paper

• Building a functional endoplasmic reticulum for proper cell growth and stress responses: a plant perspective.

  2026cites this paper
• Microautophagy: current understanding of its molecular mechanisms and functions.

  2026cites this paper
• Autophagy in Plant Health and Disease.

  2025cites this paper
• Multifaceted roles of the ATG8 protein family in plant autophagy: from autophagosome biogenesis to cargo recognition.

  2025cites this paper
• Role of autophagy in plant growth and adaptation to salt stress

  2025cites this paper
• Cross-species interactome analysis uncovers a conserved selective autophagy mechanism for protein quality control in plants.

  2025cites this paper
• The endoplasmic reticulum-associated degradation machinery selectively degrades stress-induced TIN1 during stress recovery

  2025cites this paper
• SnRK1 and TOR: central regulators of autophagy in plant energy stress responses

  2025cites this paper
• Cell-type specific autophagy in root hair forming cells is essential for salt stress tolerance in Arabidopsis thaliana

  2025cites this paper
• The TRAPPC8/TRS85 subunit of the Arabidopsis TRAPPIII tethering complex regulates endoplasmic reticulum function and autophagy

  2025cites this paper
• A RabGAP negatively regulates plant autophagy and immune trafficking.

  2024cites this paper
• Vacuolar Degradation of Plant Organelles.

  2024cites this paper
• Autophagosome biogenesis and organelle homeostasis in plant cells.

  2024cites this paper
• NBR1a mediates root-knot nematode resistance by modulating antioxidant system, jasmonic acid and selective autophagy in Solanum lycopersicum

  2024cites this paper
• The Arabidopsis thaliana TRAPPIII subunit AtTRAPPC8/AtTRS85 is involved in ER functioning and autophagy

  2024cites this paper
• Eat at Right Time: Regulations of Plant ER-phagy Receptors in Responses to Environmental Stresses.

  2024cites this paper
• Visualizing ER-phagy and ER architecture in vivo

  2024cites this paper
• Revisiting Endoplasmic Reticulum Homeostasis, an Expanding Frontier Between Host Plants and Pathogens.

  2024cites this paper
• Protein degrons and degradation: Exploring substrate recognition and pathway selection in plants.

  2024cites this paper
• Autophagy in plants

  2024cites this paper
• The S-nitrosylation of monodehydroascorbate reductase positively regulated the low temperature tolerance of mini Chinese cabbage.

  2024cites this paper
• An Overview of Protein Secretion in Plant Cells.

  2024cites this paper
• A synchronized symphony: Intersecting roles of ubiquitin proteasome system and autophagy in cellular degradation.

  2024cites this paper
• Recent advances in plant endomembrane research and new microscopical techniques.

  2023cites this paper
• Role of selective autophagy receptors in tomato response to cold stress

  2023cites this paper
• Mechanisms of Endoplasmic Reticulum Protein Homeostasis in Plants

  2023cites this paper
• Keep in contact: multiple roles of endoplasmic reticulum–membrane contact sites and the organelle interaction network in plants

  2023cites this paper
• Ufmylation reconciles salt stress-induced unfolded protein responses via ER-phagy in Arabidopsis

  2023cites this paper
• Interplay between autophagy and proteasome during protein turnover.

  2023cites this paper
• Porcine Epidemic Diarrhea Virus and Its nsp14 Suppress ER Stress Induced GRP78

  2023cites this paper
• Acyl-coenzyme A binding protein MoAcb1 regulates conidiation and pathogenicity in Magnaporthe oryzae

  2023cites this paper
• Role of endoplasmic reticulum autophagy in acute lung injury

  2023cites this paper
• Sensitive imaging of Endoplasmic reticulum (ER) autophagy with an acidity-reporting ER-Tracker

  2023cites this paper
• An Arabidopsis Rab18 GTPase promotes autophagy by tethering ATG18a to the ER in response to nutrient starvation.

  2023cites this paper
• OsHLP1 is an endoplasmic-reticulum-phagy receptor in rice plants.

  2023cites this paper
• Picky eaters: selective autophagy in plant cells.

  2023cites this paper
• The Time-Resolved Salt Stress Response of Dunaliella tertiolecta—A Comprehensive System Biology Perspective

  2023cites this paper
• Exo84c interacts with VAP27 to regulate exocytotic compartment degradation and stigma senescence

  2023cites this paper
• Hydrogen sulfide action in the regulation of plant autophagy

  2022cites this paper
• Autophagic degradation of the chloroplastic 2-phosphoglycolate phosphatase TaPGLP1 in wheat

  2022cites this paper
• Endoplasmic Reticulum Stress and Unfolded Protein Response Signaling in Plants

  2022cites this paper
• Cell Death Signaling From Endoplasmic Reticulum Stress: Plant-Specific and Conserved Features

  2022cites this paper
• Identification of New ATG8s-Binding Proteins with Canonical LC3-Interacting Region in Autophagosomes of Barley Callus.

  2022cites this paper
• ER-phagy: mechanisms, regulation, and diseases connected to the lysosomal clearance of the endoplasmic reticulum

  2022cites this paper
• ROOT HAIR DEFECTIVE3 Is a Receptor for Selective Autophagy of the Endoplasmic Reticulum in Arabidopsis

  2022cites this paper
• Plant autophagosomes mature into amphisomes prior to their delivery to the central vacuole

  2022cites this paper
• MAG2 and MAL Regulate Vesicle Trafficking and Auxin Homeostasis With Functional Redundancy

  2022cites this paper
• ER remodeling via ER-phagy

  2022influential citation
• Coordinative regulation of ERAD and selective autophagy in plants.

  2022cites this paper
• Selective autophagy: adding precision in plant immunity

  2022cites this paper
• Cargo receptors and adaptors for selective autophagy in plant cells

  2022cites this paper
• ER‐phagy: selective autophagy of the endoplasmic reticulum

  2022cites this paper
• A combination of plasma membrane sterol biosynthesis and autophagy is required for shade-induced hypocotyl elongation

  2022cites this paper
• Autophagy: a game changer for plant development and crop improvement

  2022cites this paper
• Autophagic degradation of membrane-bound organelles in plants

  2022cites this paper
• ATI1 (ATG8-interacting protein 1) and ATI2 define a plant starvation-induced reticulophagy pathway and serve as MSBP1/MAPR5 cargo receptors

  2021cites this paper
• Metabolomic profiles exhibit the influence of endoplasmic reticulum stress on sorghum seedling growth over time.

  2021cites this paper
• Mechanistic insights into an atypical interaction between ATG8 and SH3P2 in Arabidopsis thaliana

  2021cites this paper
• Advances in ER-Phagy and Its Diseases Relevance

  2021cites this paper
• Looking for a safe haven: tail-anchored proteins and their membrane insertion pathways

  2021cites this paper
• Proteasome and selective autophagy: Brothers-in-arms for organelle quality control.

  2021cites this paper
• ER-Phagy: A New Regulator of ER Homeostasis

  2021cites this paper
• Vacuolar accumulation and colocalization is not a proper criterion for cytoplasmic soluble proteins undergoing selective autophagy

  2021cites this paper
• Reduced light access promotes hypocotyl growth via autophagy-mediated recycling

  2021cites this paper
• Sec62 promotes pro-angiogenesis of hepatocellular carcinoma cells under hypoxia

  2021cites this paper
• How Lipids Contribute to Autophagosome Biogenesis, a Critical Process in Plant Responses to Stresses

  2021cites this paper
• Organelle Visualization With Multicolored Fluorescent Markers in Bamboo

  2021cites this paper
• The Role of Endoplasmic Reticulum Stress Response in Pollen Development and Heat Stress Tolerance

  2021cites this paper
• ER-phagy responses in yeast, plants, and mammalian cells and their crosstalk with UPR and ERAD.

  2021cites this paper
• Maintaining the structural and functional homeostasis of the plant endoplasmic reticulum.

  2021cites this paper
• Cargo Recognition and Function of Selective Autophagy Receptors in Plants

  2021influential citation
• ATG8-interacting (ATI) 1 and 2 define a plant starvation-induced ER-phagy pathway and serve as MSBP1 (MAPR5) cargo-receptors

  2020cites this paper
• Quantitative Proteomic Analysis of ER Stress Response Reveals both Common and Specific Features in Two Contrasting Ecotypes of Arabidopsis thaliana

  2020cites this paper
• ER-Phagy and Its Role in ER Homeostasis in Plants

  2020cites this paper
• Protein Quality Control in Plant Organelles: Current Progress and Future Perspectives.

  2020cites this paper
• New insights into AtNBR1 as a selective autophagy cargo receptor in Arabidopsis

  2020cites this paper
• AtNBR1 Is a Selective Autophagic Receptor for AtExo70E2 in Arabidopsis1[OPEN]

  2020cites this paper
• Identification and characterization of unconventional membrane protein trafficking regulators in Arabidopsis: A genetic approach.

  2020cites this paper
• Birth, Growth, Maturation, and Demise of Plant Autophagic Vesicles

  2020cites this paper
• Autophagy in plants: Physiological roles and post-translational regulation.

  2020cites this paper
• Membrane Contact Sites and Organelles Interaction in Plant Autophagy

  2020cites this paper
• Vacuole Biogenesis in Plants: How Many Vacuoles, How Many Models?

  2020cites this paper
• Reticulon proteins modulate autophagy of the endoplasmic reticulum in maize endosperm

  2020cites this paper
• The roles of endomembrane trafficking in plant abiotic stress responses.

  2019cites this paper
• Structural Biology and Electron Microscopy of the Autophagy Molecular Machinery

  2019cites this paper
• Functional Diversification of ER Stress Responses in Arabidopsis.

  2019cites this paper