Keratinase Production by Three Bacillus spp. Using Feather Meal and Whole Feather as Substrate in a Submerged Fermentation

Three Bacillus species (B. subtilis LFB-FIOCRUZ 1270, B. subtilis LFB-FIOCRUZ 1273, and B. licheniformis LFB-FIOCRUZ 1274), isolated from the poultry industry, were evaluated for keratinase production using feathers or feather meal as the sole carbon and nitrogen sources in a submerged fermentation. The three Bacillus spp. produced extracellular keratinases and peptidases after 7 days. Feather meal was the best substrate for keratinase and peptidase production in B. subtilis 1273, with 412 U/mL and 463 U/ml. The three strains were able to degrade feather meal (62–75%) and feather (40–95%) producing 3.9–4.4 mg/ml of soluble protein in feather meal medium and 1.9–3.3 mg/ml when feather medium was used. The three strains produced serine peptidases with keratinase and gelatinase activity. B. subtilis 1273 was the strain which exhibited the highest enzymatic activity.

• Publication year

  2011

• Venue

  Enzyme Research

• Publication date

  2011-08-01

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.4061/2011/523780PMID 21822479PMCID 3148598
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Separation and purification of a keratinase as pesticide against root-knot nematodes

  2011cited by this paper
• Biodegradation of feather waste by extracellular keratinases and gelatinases from Bacillus spp.

  2011cited by this paper
• Biodegradation of chicken feathers waste directed by Bacillus subtilis recombinant cells: scaling up in a laboratory scale fermentor.

  2011cited by this paper
• Optimal culture conditions for keratinase production by a novel thermophilic Myceliophthora thermophila strain GZUIFR‐H49‐1

  2011influential reference
• Medium optimization for a novel 58 kDa dimeric keratinase from Bacillus licheniformis ER-15: biochemical characterization and application in feather degradation and dehairing of hides.

  2010cited by this paper
• Purification and characterization of extreme alkaline, thermostable keratinase, and keratin disulfide reductase produced by Bacillus halodurans PPKS-2

  2010cited by this paper
• Keratinolytic activity of Bacillus subtilis AMR using human hair

  2010cited by this paper
• Production, characterization and application of keratinase from Streptomyces gulbargensis.

  2009cited by this paper
• Processing of poultry feathers by alkaline keratin hydrolyzing enzyme from Serratia sp. HPC 1383.

  2009cited by this paper
• Medium optimization for keratinase production in hair substrate by a new Bacillus subtilis KD-N2 using response surface methodology

  2009cited by this paper
• Nutritional regulation of keratinolytic activity in Bacillus pumilis

  2008cited by this paper
• Nutritional regulation of keratinolytic activity in Bacillus pumilis

  2008cited by this paper
• Purification and characterization of an alkaline keratinase from Streptomyces sp.

  2008cited by this paper
• Purification and characterization of an extracellular keratinase from a hornmeal-degrading Bacillus subtilis MTCC (9102)

  2008cited by this paper
• Degradation of raw feather by a novel high molecular weight extracellular protease from newly isolated Bacillus cereus DCUW

  2008cited by this paper
• New Feather-Degrading Filamentous Fungi

  2008cited by this paper
• Characterization of an alkaline active – thiol forming extracellular serine keratinase by the newly isolated Bacillus pumilus

  2007influential reference
• Purification and characterization of a keratinolytic metalloprotease from Chryseobacterium sp. kr6.

  2007cited by this paper
• Production of feather protein hydrolysate by keratinolytic bacterium Vibrio sp. kr2.

  2007cited by this paper
• Optimization of extracellular keratinase production by poultry farm isolate Scopulariopsis brevicaulis.

  2007cited by this paper
• A novel metalloprotease from Bacillus cereus for protein fibre processing

  2007influential reference
• Macroscopic mass and energy balance of a pilot plant anaerobic bioreactor operated under thermophilic conditions

  2006cited by this paper
• Isolation and characterization of a feather-degrading enzyme from Bacillus pseudofirmus FA30-01

  2006cited by this paper
• Microbial keratinases and their prospective applications: an overview

  2006cited by this paper
• Biochemical evidence for the proteolytic degradation of infectious prion protein PrPsc in hamster brain homogenates by foodborne bacteria.

  2006cited by this paper
• A Low-Cost Fermentation Medium for Thermophilic Protease Production by Streptomyces sp. 594 Using Feather Meal and Corn Steep Liquor

  2006cited by this paper
• Characteriztion of a novel feather-degrading Bacillus sp. strain

  2005cited by this paper
• A biotechnological process for treatment and recycling poultry feathers as a feed ingredient.

  2005cited by this paper
• Optimization of an extracellular protease of Chrysosporium keratinophilum and its potential in bioremediation of keratinic wastes

  2004cited by this paper
• Purification, characterization and immobilization of a keratinase from Aspergillus oryzae

  2004cited by this paper
• Novel alkaline proteases from alkaliphilic bacteria grown on chicken feather

  2003influential reference
• Keratin degradation: a cooperative action of two enzymes from Stenotrophomonas sp.

  2002cited by this paper
• Characterization of a Keratinolytic Serine Protease from Bacillus subtilis KS-1

  2001cited by this paper
• Simplified endoproteinase assays using gelatin or azogelatin.

  1998cited by this paper
• Nucleotide sequence and expression of kerA, the gene encoding a keratinolytic protease of Bacillus licheniformis PWD-1

  1995cited by this paper
• Comparative characterization of proteolytic enzymes from Trichophyton gallinae and Trichophyton verrucosum.

  1989cited by this paper
• Intracellular keratinase of Trichophyton gallinae.

  1987influential reference
• Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

  1970cited by this paper
• Protein measurement with the Folin phenol reagent.

  1951cited by this paper

• Couple action of some proteases from feather keratin-degrading bacterium B.licheniformis CP-16.

  2026cites this paper
• The potential of hydrolyzed chicken feather meal as a partial replacement for fish meal and its effects on the growth and health status of African catfish (Clarias gariepinus) fingerlings

  2025cites this paper
• Evaluation of Bacillus-based direct fed microbials on the performance and eggshell quality of first cycle laying hens from 55 to 75 weeks of age

  2025cites this paper
• Harnessing the potential of microbial keratinases for bioconversion of keratin waste

  2024cites this paper
• Physical treatment and protease or probiotic supplementation and feather meal digestibility by broilers

  2024cites this paper
• Isolation, identification, and molecular characterization of potential keratinolytic fungus sp. from Southern Assam: relevance to poultry wastes and its biological management

  2024influential citation
• STUDY OF OPTIMIZATION, CHARACTERIZATION AND APPLICATIONS OF KERATINASE PRODUCED BY A BACILLUS STRAIN

  2024cites this paper
• Characterization of a Bacterial Keratinolytic Protease for Effective Degradation of Chicken Feather Waste into Feather Protein Hydrolysates.

  2024influential citation
• A Review of Poultry Waste-to-Wealth: Technological Progress, Modeling and Simulation Studies, and Economic- Environmental and Social Sustainability

  2023cites this paper
• Growth performance and meat quality of broiler chickens on diets containing Keratinase-treated and untreated feather meal-based diets

  2023cites this paper
• Poultry waste management in Nigeria: a neglected sector

  2023cites this paper
• Optimize and Purification of Keratinase Produced from Local Aspergillus Terreus A13 Isolate Using A Feather as Substrate

  2023influential citation
• Valorization of chicken feather into organic liquid fertilizer through two species of Bacillus bacteria fermentation

  2023cites this paper
• Characterizing hydrolysate from duck feather degradation by Pseudomonas sp. PK4, Bacillus cereus TD5B, and Bacillus cereus LS2B

  2023cites this paper
• The Novelty of Amino Acid Production From Feathers Degradation Em-ploying Keratinase from Streptomyces Venezuelae AZ15

  2023cites this paper
• Endophytes Producing Proteases from Custard Apple (Annona squamosa L.) Leaves

  2023cites this paper
• Keratinase Production by Bacillus sp. MD24 in Sub-merge and Solid State Fermentation

  2023cites this paper
• Isolation of keratinase-producing Bacillus strains and enhanced enzyme production using in vitro mutagenesis.

  2022cites this paper
• Optimization of feather degradation by a Bacillus thuringiensis isolate using response surface methodology and investigation of the feather protein hydrolysate structure

  2022cites this paper
• Isolation, Characterization, and Optimization of Keratinase from Bacillus cereus BRAW_KM

  2022cites this paper
• Valorization of Feather by Bacillus safensis and Aquamicrobium defluvii for Growth Promotion in Leafy Vegetables

  2022cites this paper
• Enzymatic activity and amino acid production by indigenous keratinolytic strains on the various poultry feather substrate

  2022cites this paper
• Development and Evaluation of a Commercial Direct-Fed Microbial (Zymospore®) on the Fecal Microbiome and Growth Performance of Broiler Chickens under Experimental Challenge Conditions

  2022cites this paper
• Micrococcus luteus : A Potential Keratinolytic Bacteria Isolated from Poultry Waste Dumping Site at Namakkal District, Tamil Nadu, India International Journal of Zoological Investigations

  2022cites this paper
• Feather-Degrading Bacillus cereus HD1: Genomic Analysis and Its Optimization for Keratinase Production and Feather Degradation

  2022cites this paper
• Alternatives to Antimicrobial Growth Promoters and Their Impact in Gut Microbiota, Health and Disease: Volume II

  2022cites this paper
• Potential of keratinolytic fungi isolated from chicken and goat farms soil, North Sumatra

  2022cites this paper
• Greener and Sustainable Biovalorization of Poultry Waste into Peptone via Bacto-Enzymatic Digestion: A Breakthrough Chemical-Free Bioeconomy Waste Management Approach

  2022cites this paper
• Hydrolyzed feather keratin obtained by microbial fermentation encapsulated with maltodextrin – A sustainable approach to increase digestible protein in feed

  2022cites this paper
• Novel Feather Degrading Keratinases from Bacillus cereus Group: Biochemical, Genetic and Bioinformatics Analysis

  2022cites this paper
• Immobilization and Biochemical Characterization of Keratinase 2S1 onto Magnetic Cross-Linked Enzyme Aggregates and its Application on the Hydrolysis of Keratin Waste

  2021cites this paper
• The Use of Microorganism-Derived Enzymes for Bioremediation of Soil Pollutants

  2021cites this paper
• Efficiency of polymer/salt aqueous two-phase electrophoresis system for recovery of extracellular Kytococcus sedentarius TWHKC01 keratinase

  2021cites this paper
• Use of chicken feather meal fermented with Bacillus subtilis in diets to increase the digestive enzymes activity and nutrient digestibility of silver pompano Trachinotus blochii (Lacepede, 1801)

  2021cites this paper
• Molecular strategies to increase keratinase production in heterologous expression systems for industrial applications

  2021cites this paper
• Microbial Hydrolysed Feather Protein as a Source of Amino Acids and Protein in the Diets of Animals Including Poultry

  2021cites this paper
• Advances in Use of Keratinase from Feather Wastes for Feedstock Modification

  2021cites this paper
• Analysis and Characterization of Solid and Liquid Organic Fertilizer from Hydrothermal Carbonization (HTC) of Chicken Feather and Blood Waste

  2021cites this paper
• Bioconversion of animal hair waste using salt- and sulphide-tolerant Bacillus sp. KLP1 and depilation using keratinase

  2021cites this paper
• Screening of Enzyme Producers with Keratinase Activity among Marine Actinobacteria

  2021cites this paper
• INFLUENCE OF PROFORT FEED ADDITIVE ON MEAT PRODUCTIVITY OF TURKEY OF «HYBRID CONVERTER» CROSS

  2021cites this paper
• Agro-industrial by-products as substrates for the production of bacterial protease and antioxidant hydrolysates

  2021cites this paper
• Genomic characterization and production of antimicrobial lipopeptides by Bacillus velezensis P45 growing on feather by‐products

  2021cites this paper
• Determination of Keratinase Enzyme Activity by Bacillus megatrium SKH14 Strain

  2021cites this paper
• PENENTUAN KONDISI OPTIMUM PRODUKSI ENZIM KERATINASE OLEH Actinobacillus spp. MENGGUNAKAN TEPUNG BULU AYAM SEBAGAI SUBSTRAT PADA FERMENTASI MEDIA CAIR

  2021influential citation
• Arthroderma tuberculatum and Arthroderma multifidum Isolated from Soils in Rook (Corvus frugilegus) Colonies as Producers of Keratinolytic Enzymes and Mineral Forms of N and S

  2020cites this paper
• Microbial keratinases: An overview of biochemical characterization and its eco-friendly approach for industrial applications

  2020cites this paper
• Bacillus cytotoxicus Isolated from a Pristine Natural Geothermal Area Reveals High Keratinolytic Activity

  2020cites this paper
• Organic Fertilizer Source and Application Method Impact Ammonia Volatilization

  2020cites this paper
• Optimisation and production of alpha amylase from thermophilic Bacillus spp. and its application in food waste biodegradation

  2020cites this paper
• Utilization of feather meal fermented Bacillus subtilis to replace fish meal in the diet of silver pompano, Trachinotus blochii (Lacepede, 1801).

  2020cites this paper
• Microbial Bioremediation of Feather Waste for Keratinase Production: An Outstanding Solution for Leather Dehairing in Tanneries

  2020cites this paper
• Administration of Bacillus cultures as direct-fed microorganisms improves growth performance and reduces mortality of broilers raised under performance-reducing stress challenge

  2020cites this paper
• Response Surface Methodology for the Optimization of Keratinase Production in Culture Medium Containing Feathers by Bacillus sp. UPM-AAG1

  2020cites this paper
• Bioutilization of poultry feather for keratinase production and its application in leather industry

  2019cites this paper
• OF THE PAKISTAN ACADEMY OF SCIENCES : B . Life and Environmental Sciences

  2019cites this paper
• Development and characterization of photoprotective formulations containing keratin particles

  2019cites this paper
• Alkaline Protease: A Tool to Manage Solid Waste and Its Utility in Detergent Industry

  2019cites this paper
• Co-production of Proteases and Bioactive Protein Hydrolysates from Bioprocessing of Feather Meal

  2019cites this paper
• The Use of Probiotics in Poultry Production for the Control of Bacterial Infections and Aflatoxins

  2019cites this paper
• The effects of chicken feather silage substitution for fish meal in the diet on growth of saline tilapia fingerlings (Oreochromis niloticus)

  2019cites this paper
• Microbial production and industrial applications of keratinases: an overview

  2018cites this paper
• Bioconversion of chicken feathers by Bacillus aerius NSMk2: A potential approach in poultry waste management

  2018cites this paper
• Efficacy of crude and immobilizedenzymes from Bacillus licheniformis for production of biodegraded feather meal and their assessment on chickens

  2018cites this paper
• Biodegradation of Feather Waste Keratin by the Keratin-Degrading Strain Bacillus subtilis 8.

  2018cites this paper
• Striking Applications of Keratinase Enzyme Isolated from Various Natural Sources: A Review:

  2018cites this paper
• Keratinolytic activity of Bacillus subtilis LFB-FIOCRUZ 1266 enhanced by whole-cell mutagenesis

  2018cites this paper
• Profile of Turbidity and Glucose Formation from Underutilised Wild, Edible Bean during In-Vitro Gastro Intestinal Digestion and Fermentation

  2018cites this paper
• PURIFICATION AND CHARACTERIZATION OF NOVEL EXTRACELLULAR KERATINASE ENZYME FROM POULTRY FEATHER WASTE

  2018cites this paper
• Keratinase from newly isolated strain of thermophilic Bacillus for chicken feed modification

  2017cites this paper
• Production, partial optimization and characterization of keratinase enzyme by Arthrobacter sp. NFH5 isolated from soil samples

  2017cites this paper
• The 6 th ISTAP International Seminar on Tropical Animal Production “ Integrated Approach in Developing Sustainable Tropical Animal Production

  2017cites this paper
• Aktywność proteolityczna keratynolitycznych bakterii Stenotrophomonas rhizophila

  2017cites this paper
• Estimation of metabolizable energy equivalency of Bacillus Subtilis spore for male broiler chickens

  2017cites this paper
• Purification and characterization of a keratinolytic protease produced by probiotic Bacillus subtilis

  2016cites this paper
• Evaluation and Selection of Bacillus Species Based on Enzyme Production, Antimicrobial Activity, and Biofilm Synthesis as Direct-Fed Microbial Candidates for Poultry

  2016cites this paper
• Biocatalysis of Keratin-Based Waste as A Source Of Soluble Protein

  2016cites this paper
• Evaluation and Selection of a Bacillus based Direct-Fed Microbial Candidate for In situ Enzyme Production to Improve Gut Health Integrity, Bone Quality and Growth Performance in Poultry

  2016cites this paper
• In vitro and in vivo evaluation of protein quality of enzymatic treated feather meals

  2016cites this paper
• Organic nitrogen fertilizers influence nutritional value, water use efficiency, and nitrogen dynamics of drip irrigated lettuce and sweet corn

  2016cites this paper
• Microflora in the continental shelf sediments of south western Bay of Bengal

  2016cites this paper
• ARTICLE; AGRICULTURE AND ENVIRONMENTAL BIOTECHNOLOGY Bacillus safensis LAU 13: a new source of keratinase and its multi-functional biocatalytic applications

  2015cites this paper
• A Statistical-Mathematical Model to Optimize Chicken Feather Waste Bioconversion via Bacillus licheniformis SHG10: A Low Cost Effective and Ecologically Safe Approach

  2015cites this paper
• Enhanced Production, Purification and Characterization of Alkaline Keratinase from Streptomyces minutiscleroticus DNA38

  2015influential citation
• Purification and characterization of four key enzymes from a feather-degrading Bacillus subtilis from the gut of tarantula Chilobrachys guangxiensis

  2014cites this paper
• Hydrolyzing Proficiency of Keratinases in Feather Degradation

  2014cites this paper
• Inhibitory effect of linalool-rich essential oil from Lippia alba on the peptidase and keratinase activities of dermatophytes

  2014cites this paper
• Valorization of Chicken Feather Waste for Concomitant Production of Keratinase, Oligopeptides and Essential Amino Acids Under Submerged Fermentation by Paenibacillus woosongensis TKB2

  2014cites this paper
• Evaluation of germination, distribution, and persistence of Bacillus subtilis spores through the gastrointestinal tract of chickens.

  2014cites this paper
• Valorization of Chicken Feather Waste for Concomitant Production of Keratinase, Oligopeptides and Essential Amino Acids Under Submerged Fermentation by Paenibacillus woosongensis TKB2

  2013cites this paper
• Optimization of Keratinase Production for Feather Degradation by Bacillus subtilis

  2013cites this paper
• Potential of chicken by-products as sources of useful biological resources.

  2013cites this paper
• A Potential Strain of Keratinolytic Bacteria VIT RSAS 2 from Katpadi and Its Pharmacological Benefits

  2013cites this paper
• Abstrak— Genus Bacillus merupakan bakteri penghasil enzim keratinase yang mampu memutus ikatan disulfida pada protein keratin menjadi protein terlarut. Keratin adalah protein yang menyusun struktur kulit, rambut, bulu, kuku, tanduk, dan struktur luar vertebrata lainnya yang bersifat tidak larut. Tuj

  2013cites this paper
• Feather keratin hydrolysates obtained from microbial keratinases: effect on hair fiber

  2013cites this paper
• Desempeño productivo y rendimiento de canal en pavos alimentados con harina de plumas tratadas con NaOH

  2013cites this paper
• Zymogram Analysis of Alkaline Keratinase Produced by Nitrogen Fixing Bacillus pumilus ZED17 Exhibiting Multiprotease Enzyme Activities

  2013cites this paper
• BiochemicalandMolecular CharacterizationofMicrobial KeratinaseandIts Remarkable Applications

  2012cites this paper
• Biochemical and Molecular Characterization of Microbial Keratinase and Its Remarkable Applications

  2012cites this paper
• A review on optimizing fish growth using fermented animal by-products for sustainable aquaculture

  year unknowncites this paper