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We are ready for faunistic surveys of bdelloid rotifers through DNA barcoding: the example of Sphagnum bogs of the Swiss Jura Mountains

D. Fontaneto,E. Eckert,Nikoleta Anicic,Enrique Lara,E. Mitchell

Published 2019 in Limnetica

ABSTRACT

espanolLa identificacion de la diversidad biologica a traves de DNA barcoding y metabarcoding de los organismos en el medio ambiente tiene el potencial de revolucionar la forma en que se realizan los inventarios biologicos y el monitoreo. Sin embargo, todavia no se sabe si las bases de datos geneticos de referencia a disposicion hoy en dia son lo suficientemente representativas como para permitir tales enfoques. Aqui, mostramos que, al menos para los rotiferos bdelloideos (Metazoa; Rotifera; Bdelloidea) de Europa, el nivel de conocimiento es suficiente para realizar tales estudios. Mostramos los resultados de un ejercicio realizado sobre rotiferos bdelloideos en turberas de Sphagnum del Jura suizo. Los resultados de las identificaciones basadas en el ADN fueron bastante consistentes con las identificaciones basadas en la morfologia, y los pocos casos de desajuste podrian utilizarse como una advertencia para evitar posibles interpretaciones erroneas de los resultados. Estos desajustes se debieron a que las secuencias mas cercanas seguian alejadas de los organismos realmente encontrados y a la presencia de especies cripticas EnglishThe identification of biological diversity through DNA barcoding and metabarcoding of the organisms living in the field has the potential to revolutionise the way biological surveys and monitoring are performed. Yet, we still do not know if the current representativeness of the reference database of DNA sequence data is sufficient to allow such approaches. Here, we show that, at least for bdelloid rotifers (Metazoa; Rotifera; Bdelloidea) in Europe, current knowledge is ripe to perform such surveys. We show the results of an exercise performed on bdelloid rotifers in Sphagnum bogs of the Swiss Jura Mountain. The results of DNA-based identifications were rather consistent with the morphology-based identifications, and the few cases of mismatch could be used as a cautionary tale to avoid potential misinterpretations of results. The mismatches were due to cases of the closest match not being genetically very close, and to the occurrence of cryptic species.

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