Increased torulene production by the red yeast, Sporidiobolus pararoseus, using citrus juice

Abstract Response surface methodology was applied to maximize the yield and production of carotenoids by Sporidiobolus pararoseus WZ012 using citrus juice. A high concentration of yeast extract and citrus juice favored carotenoid production and biomass concentration, respectively. Under optimal conditions, a more than 51 percent (from 860 to 1300 µg/g) and 62 percent (from 17.05 to 27.66 mg/L) respective enhancement in intracellular and total carotenoid production was achieved. Finally, this process was successfully upscaled in a 5-L fermentor. A comparison of the carotenoid distributions revealed that torulene (61.3%) was the dominant carotenoid when using the citrus based medium, while the main carotenoid was β-carotene (62.5%) when using the glucose medium. The present work provides an alternative method to produce high-value products derived from waste and low-grade citrus.

• Publication year

  2020

• Venue

  Preparative Biochemistry & Biotechnology

• Publication date

  2020-01-02

• Fields of study

  Medicine, Chemistry, Environmental Science

• Identifiers
  DOI 10.1080/10826068.2019.1663533PMID 31502910
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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• Formation of carotenoids by rhodotorula glutinis in whey ultrafiltrate

  1994cited by this paper
• High-performance liquid chromatographic analysis of major carotenoids from Rhodotorula glutinis

  1988influential reference
• Carbohydrate analysis: a practical approach

  1986cited by this paper
• Protein measurement with the Folin phenol reagent.

  1951cited by this paper

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