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Inter- and Intraspecific Variation in Sex Hormone-Induced Sex-Reversal in Medaka, Oryzias latipes and Oryzias sakaizumii

T. Myosho,Tadashi Sato,Hiroka Nishiyama,Akiho Watanabe,Jun Yamamoto,T. Okamura,Yuta Onishi,R. Fujimaki,S. Hamaguchi,M. Sakaizumi,Tohru Kobayashi

Published 2019 in Zoological Science

ABSTRACT

We compared sex-reversal ratios induced by 17α-methyltestosterone (MT) and 17β-estradiol (E2) exposure in two inbred medaka strains: Hd-rR derived from Oryzias latipes and HNI-II from O. sakaizumii. All MT exposures (0.2–25 ng mL–1) induced complete XX sex-reversal in HNI-II. Although MT exposure at 0.2 ng mL–1 induced XX sex-reversal at > 95% in Hd-rR, other concentrations tested caused XX sex-reversal at lower frequencies (<50%). MT exposure at 1, 5, and 25 ng mL–1 induced XY sex-reversal in Hd-rR, but not in HNI-II. In Hd-rR, E2 exposure induced XY sex-reversal at > 10 ng mL–1, and in all fish feminization occurred 500 ng mL–1. In HNI-II, E2 induced XY sex-reversal at 50 and 250 ng mL–1, but only at rates below 20%. To clarify whether the strain differences in sex hormone-induced sex-reversal are characteristic of each species, we examined the effects of MT and E2 exposure on sex differentiation in five and two additional strains or wild stocks/populations of O. latipes and O. sakaizumii, respectively. MT exposure induced low XX and high XY sex-reversal rates in O. latipes, except in the Shizuoka population, but the trend was reversed in O. sakaizumii. Furthermore, E2-induced XY sex-reversal rates varied intraspecifically in O. latipes. Our results demonstrated that sensitivity to MT and E2 varied within O. latipes species. To evaluate the ecological impacts of environmental chemicals using medaka, it is important to define not only the species, but the strains, stocks, and populations to obtain accurate results.

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