Organic molecule-assisted synthesis of Fe3O4/graphene oxide nanocomposites for selective capture of low-abundance peptides and phosphopeptides.

The iron oxide nanoparticles (Fe3O4) were prepared by organic molecule-assisted method in aqueous solution. The facile synthetic process of Fe3O4 nanoparticles was conducted only by mixing FeCl2 and 2-methylimidazole (2-MIM) without any additives. A possible growth mechanism of the Fe3O4 nanocrystals was proposed for this mild reaction. Then, the Fe3O4 nanoparticles were anchored onto graphene oxide (GO) sheets in water by ultrasound-assisted method, forming an affinity probe with strong biocompatibility. Due to the hydroxy and carboxylic groups of GO sheets, Fe3O4/GO probe exhibits excellent performance for enriching low-abundance hydrophilic peptides, while the Fe3O4 nanoparticles endure the probe with specific affinity to phosphopeptides. The analytical protocol was developed for sequential enrichment of low-abundance peptides and phosphopeptides by the affinity probe. It exhibited the sequence coverage of 26% for capture of 17 low-abundance peptides from bovine serum albumin (BSA), as well as the selectivity of 1:1:100 for phosphopeptides from α-/β-casein/BSA, and low detectable concentration of 2.5 fmol and probe reusability of 5 times for capture of phosphopeptides from α-/β-casein. Consequently, the prepared Fe3O4/GO material possesses excellent feature as multifunctional affinity probe for low-abundance peptides including phosphopeptides from complex biological matrices detected by matrix-assisted laser desorption ionization time-of-flight mass spectrometry.

• Publication year

  2020

• Venue

  Talanta: The International Journal of Pure and Applied Analytical Chemistry

• Publication date

  2020-02-01

• Fields of study

  Medicine, Materials Science, Chemistry

• Identifiers
  DOI 10.1016/j.talanta.2019.120437PMID 31816680
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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